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AB110294

Anti-ECH1 antibody [9D11AF3]

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(1 Publication)

Mouse Monoclonal ECH1 antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ECH1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-ECH1 antibody [9D11AF3] (AB110294)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ECH1 antibody [9D11AF3] (AB110294)

Immunocytochemistry image of ab110294 stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with the antibody (9D11AF3, 4 µg/ml) for 2h at room temperature or over night at 4°C. The secondary antibody was (green) AlexaFluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in the mitochondria.

Flow Cytometry - Anti-ECH1 antibody [9D11AF3] (AB110294)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-ECH1 antibody [9D11AF3] (AB110294)

HL-60 cells were stained with 1 µg/mL ab110294 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

9D11AF3

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, ICC/IF

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "4 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ECH1 is a member of the hydratase/isomerase superfamily involved in fatty acid metabolism. It is localized to peroxisomes and/or mitochondria in different mammals. In humans, levels of ECH1 are modulated by peroxisome proliferators. As the name implies, ECH1 catalyzes the isomerization of cis Δ3,5, double bonds in unsaturated acyl-CoAs to yield trans Δ2,4-dienoyl CoAs. These are substrates for Δ2,4-dienoyl CoA reductase which produces trans-3 enoyl CoA. This intermediate can then be further transformed by Δ3, Δ2 - enoyl CoA isomerase to finally yield the trans-2 double bond configured fatty acyl CoA, which can be then directed into the ß-oxidation pathway. Expression of ECH1 is highest in metabolically active tissues such as heart, skeletal muscle, central nervous system, liver and kidney. Recent studies show that it is down regulated in gastric carcinoma and CLL.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Precipitation Ammonium Sulphate
Purification notes
Near homogeneity as judged by SDS-PAGE. ab110294 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Storage buffer
pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ECH1 also known as Delta(35)-Delta(24)-dienoyl-CoA isomerase is a mitochondrial protein with a mass of approximately 31 kDa. It is expressed in a wide variety of tissues including high levels in the liver heart and muscle. This protein plays an important role in the beta-oxidation of unsaturated fatty acids inside mitochondria by catalyzing the conversion of 35-dienoyl-CoA to 24-dienoyl-CoA. Because of this function ECH1 contributes to the efficient metabolism of fatty acids.
Biological function summary

The ECH1 protein facilitates the breakdown and utilization of unsaturated fatty acids thereby providing energy for cellular processes. It acts independently and is not a part of a large protein complex. Mitochondria rely on ECH1 to handle specific intermediates in fatty acid metabolism which impacts how cells manage their energy resources. This makes ECH1 essential for maintaining energy homeostasis particularly during periods of increased metabolic demand.

Pathways

ECH1 is integral to the mitochondrial fatty acid beta-oxidation pathway. This pathway is important for converting fatty acids into acetyl-CoA units which then enter the Krebs cycle for further energy production. ECH1 interacts with other proteins like ACAA2 which contributes to the final step of fatty acid degradation. Alongside this it aligns with the peroxisomal biogenesis pathway influencing the balance between metabolic processes within mitochondria and peroxisomes.

ECH1 activity impacts conditions such as metabolic syndrome and inherited disorders of mitochondrial fatty acid oxidation including Medium-chain acyl-CoA dehydrogenase (MCAD) deficiency. Changes in the function or expression of ECH1 can lead to accumulation of fatty acid intermediates causing cellular dysfunction. In diseases where energy balance is disrupted like metabolic syndrome ECH1's interaction with proteins like HADHA which assists in the final steps of beta-oxidation becomes significant for understanding and potentially targeting treatments.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Isomerization of 3-trans,5-cis-dienoyl-CoA (3E,5Z-dienoyl-CoA) to 2-trans,4-trans-dienoyl-CoA (2E,4E-dienoyl-CoA), a crucial step in the beta-oxidation of unsaturated fatty acids with double bonds at odd position.
See full target information Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 21:725-744 PubMed39781464

2025

Mitochondrial SIRT2-mediated CPT2 deacetylation prevents diabetic cardiomyopathy by impeding cardiac fatty acid oxidation.

Applications

Unspecified application

Species

Unspecified reactive species

Yaoyao Guo,Ziyin Zhang,Zheng Wen,Xiaonan Kang,Dan Wang,Lu Zhang,Mengke Cheng,Gang Yuan,Huihui Ren
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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