AB153720

Anti-ECH1 antibody

Name: Anti-ECH1 antibody (ab153720) | Abcam
Brand: Abcam Limited
SKU: AB153720
Price: 510 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(6 Publications)

Rabbit Polyclonal ECH1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial aa 50 to C-terminus.

View Alternative Names

ECH1

4 Images

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ECH1 antibody (AB153720)

Confocal immunofluorescent analysis of methanol-fixed HeLa cells, labeling ECH1 with ab153720 at 1/500 dilution (green). Alpha-tubulin filaments labeled in red.

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Western blot - Anti-ECH1 antibody (AB153720)

10% SDS PAGE

All lanes:

Western blot - Anti-ECH1 antibody (ab153720) at 1/1000 dilution

All lanes:

A549 whole cell lysate at 30 µg

Predicted band size: 35 kDa,36 kDa

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Western blot - Anti-ECH1 antibody (AB153720)

10% SDS PAGE

All lanes:

Western blot - Anti-ECH1 antibody (ab153720) at 1/1000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 36 kDa

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Lab

Western blot - Anti-ECH1 antibody (AB153720)

All lanes:

Western blot - Anti-ECH1 antibody (ab153720) at 1/2000 dilution

Lane 1:

WT 293T cell extracts at 30 µg

Lane 2:

ECH1 Knockout 293T cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 36 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q13011

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ECH1 also known as Delta(35)-Delta(24)-dienoyl-CoA isomerase is a mitochondrial protein with a mass of approximately 31 kDa. It is expressed in a wide variety of tissues including high levels in the liver heart and muscle. This protein plays an important role in the beta-oxidation of unsaturated fatty acids inside mitochondria by catalyzing the conversion of 35-dienoyl-CoA to 24-dienoyl-CoA. Because of this function ECH1 contributes to the efficient metabolism of fatty acids.

Biological function summary

The ECH1 protein facilitates the breakdown and utilization of unsaturated fatty acids thereby providing energy for cellular processes. It acts independently and is not a part of a large protein complex. Mitochondria rely on ECH1 to handle specific intermediates in fatty acid metabolism which impacts how cells manage their energy resources. This makes ECH1 essential for maintaining energy homeostasis particularly during periods of increased metabolic demand.

Pathways

ECH1 is integral to the mitochondrial fatty acid beta-oxidation pathway. This pathway is important for converting fatty acids into acetyl-CoA units which then enter the Krebs cycle for further energy production. ECH1 interacts with other proteins like ACAA2 which contributes to the final step of fatty acid degradation. Alongside this it aligns with the peroxisomal biogenesis pathway influencing the balance between metabolic processes within mitochondria and peroxisomes.

ECH1 activity impacts conditions such as metabolic syndrome and inherited disorders of mitochondrial fatty acid oxidation including Medium-chain acyl-CoA dehydrogenase (MCAD) deficiency. Changes in the function or expression of ECH1 can lead to accumulation of fatty acid intermediates causing cellular dysfunction. In diseases where energy balance is disrupted like metabolic syndrome ECH1's interaction with proteins like HADHA which assists in the final steps of beta-oxidation becomes significant for understanding and potentially targeting treatments.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Isomerization of 3-trans,5-cis-dienoyl-CoA (3E,5Z-dienoyl-CoA) to 2-trans,4-trans-dienoyl-CoA (2E,4E-dienoyl-CoA), a crucial step in the beta-oxidation of unsaturated fatty acids with double bonds at odd position.

See full target information Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell communication and signaling 18:e12038 PubMed38946717

2024

Enoyl coenzyme a hydratase 1 attenuates aortic valve calcification by suppressing Runx2 via Wnt5a/Ca pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Caijun Rao,Baoqing Liu,Haojie Qin,Zhipeng Du

Cells 10: PubMed34944023

2021

The Methyltransferase Smyd1 Mediates LPS-Triggered Up-Regulation of IL-6 in Endothelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed Shamloul,Gustav Steinemann,Kerrin Roos,Celine Huajia Liem,Jonathan Bernd,Thorsten Braun,Andreas Zakrzewicz,Janine Berkholz

Autophagy 17:3753-3762 PubMed33685343

2021

Mt-Keima detects PINK1-PRKN mitophagy with greater sensitivity than mito-QC.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Ting Liu,Danielle A Sliter,Mario K Shammas,Xiaoping Huang,Chunxin Wang,Hannah Calvelli,Dragan S Maric,Derek P Narendra

Metabolism: clinical and experimental 114:154416 PubMed33137378

2020

Pioglitazone corrects dysregulation of skeletal muscle mitochondrial proteins involved in ATP synthesis in type 2 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Teresa Vanessa Fiorentino,Adriana Monroy,Subash Kamath,Rosa Sotero,Michele Dei Cas,Giuseppe Daniele,Alberto O Chavez,Muhammad Abdul-Ghani,Marta Letizia Hribal,Giorgio Sesti,Devjit Tripathy,Ralph A DeFronzo,Franco Folli

American journal of physiology. Endocrinology and 318:E318-E329 PubMed31961704

2020

Enoyl coenzyme A hydratase 1 combats obesity and related metabolic disorders by promoting adipose tissue browning.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoxiang Mao,Dandan Huang,Caijun Rao,Meng Du,Minglu Liang,Fei Li,Baoqing Liu,Kai Huang

Thyroid : official journal of the American Thyroid Association 29:845-857 PubMed30990120

2019

Reprogramming of Energy Metabolism: Increased Expression and Roles of Pyruvate Carboxylase in Papillary Thyroid Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Aurélie Strickaert,Cyril Corbet,Selim-Alex Spinette,Ligia Craciun,Geneviève Dom,Guy Andry,Denis Larsimont,Ruddy Wattiez,Jacques E Dumont,Olivier Feron,Carine Maenhaut

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ECH1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ECH1 antibody (AB153720)

Western blot - Anti-ECH1 antibody (AB153720)

Western blot - Anti-ECH1 antibody (AB153720)

Western blot - Anti-ECH1 antibody (AB153720)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise