AB189255

Anti-ECH1 antibody [EPR15449(B)] - C-terminal

RabMAb
Recombinant
KO Validated
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(2 Publications)

Rabbit Recombinant Monoclonal ECH1 antibody. C-terminal. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 2 publications.

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Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (AB189255)

Intracellular flow cytometric analysis of2% paraformaldehyde-fixed Jurkat cells labeling ECH1 with ab189255 at 1/20 dilution (red) compared to aRabbit monoclonal IgG isotype control (green), followed byGoat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (AB189255)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (AB189255)

Immunhistochemical analysis of paraffin-embedded human kidney tissue labeling ECH1 with ab189254 at 1/500 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (AB189255)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (AB189255)

Immunfluorescent analysis of 100% methanol(-20℃)-fixed MCF-7 cells labeling ECH1 with ab189254 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with DAPI.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (AB189255)

Immunhistochemical analysis of paraffin-embedded human endometrium tissue labeling ECH1 with ab189254 at 1/500 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (AB189255)

Western blot analysis of ECH1 in human fetal liver lysate immunoprecipitated using ab189254 at 1/50 dilution.

Secondary antibody : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)

Predicted band size: 36 kDa

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Lab

Western blot - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (AB189255)

Lanes 1-4 : Merged signal (red and green). Green - ab189255 observed at 36 kDa. Red - loading control ab7291 observed at 50 kDa.

ab189255 Anti-ECH1 antibody [EPR15449(B)] was shown to specifically react with ECH1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266748 (knockout cell lysate ab257933) was used. Wild-type and ECH1 knockout samples were subjected to SDS-PAGE. ab189255 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

ECH1 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human ECH1 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-ech1-knockout-hek-293t-cell-line-ab266748'>ab266748</a>)

Lane 3:

A549 cell lysate at 20 µg

Lane 4:

U-2 OS cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 36 kDa,80 kDa

Observed band size: 100 kDa,36 kDa

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Supplier Data

Western blot - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (AB189255)

All lanes:

Western blot - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255) at 1/50000 dilution

Lane 1:

Human fetal liver lysate at 20 µg

Lane 2:

A549 cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 36 kDa

Observed band size: 33 kDa

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Carrier free

Anti-ECH1 antibody [EPR15449(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15449(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra), WB, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/60", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ECH1 also known as Delta(35)-Delta(24)-dienoyl-CoA isomerase is a mitochondrial protein with a mass of approximately 31 kDa. It is expressed in a wide variety of tissues including high levels in the liver heart and muscle. This protein plays an important role in the beta-oxidation of unsaturated fatty acids inside mitochondria by catalyzing the conversion of 35-dienoyl-CoA to 24-dienoyl-CoA. Because of this function ECH1 contributes to the efficient metabolism of fatty acids.

Biological function summary

The ECH1 protein facilitates the breakdown and utilization of unsaturated fatty acids thereby providing energy for cellular processes. It acts independently and is not a part of a large protein complex. Mitochondria rely on ECH1 to handle specific intermediates in fatty acid metabolism which impacts how cells manage their energy resources. This makes ECH1 essential for maintaining energy homeostasis particularly during periods of increased metabolic demand.

Pathways

ECH1 is integral to the mitochondrial fatty acid beta-oxidation pathway. This pathway is important for converting fatty acids into acetyl-CoA units which then enter the Krebs cycle for further energy production. ECH1 interacts with other proteins like ACAA2 which contributes to the final step of fatty acid degradation. Alongside this it aligns with the peroxisomal biogenesis pathway influencing the balance between metabolic processes within mitochondria and peroxisomes.

ECH1 activity impacts conditions such as metabolic syndrome and inherited disorders of mitochondrial fatty acid oxidation including Medium-chain acyl-CoA dehydrogenase (MCAD) deficiency. Changes in the function or expression of ECH1 can lead to accumulation of fatty acid intermediates causing cellular dysfunction. In diseases where energy balance is disrupted like metabolic syndrome ECH1's interaction with proteins like HADHA which assists in the final steps of beta-oxidation becomes significant for understanding and potentially targeting treatments.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Isomerization of 3-trans,5-cis-dienoyl-CoA (3E,5Z-dienoyl-CoA) to 2-trans,4-trans-dienoyl-CoA (2E,4E-dienoyl-CoA), a crucial step in the beta-oxidation of unsaturated fatty acids with double bonds at odd position.

See full target information Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Clinical and experimental medicine 25:115 PubMed40214825

2025

TPM4 influences the initiation and progression of gastric cancer by modulating ferroptosis via SCD1.

Applications

Unspecified application

Species

Unspecified reactive species

Ling-Lin Zhao,Yu-Jun Liu,Qi-Jing Guo,Nan Yan,Jie Yang,Jing-Qi Han,Xiao-Hong Xie,Yu-Shuang Luo

Nucleic acids research 53: PubMed40183632

2025

AOC 1044 induces exon 44 skipping and restores dystrophin protein in preclinical models of Duchenne muscular dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Usue Etxaniz,Isaac Marks,Tyler Albin,Matthew Diaz,Raghav Bhardwaj,Aaron Anderson,Olecya Tyaglo,Tiffany Hoang,Maria Azzurra Missinato,Kristoffer Svensson,Ben Badillo,Philip R Kovach,Laura Leung,Michael Cochran,Hae Won Kwon,Md Nur Ahad Shah,Rika Maruyama,Toshifumi Yokota,Venkata R Doppalapudi,Beatrice Darimont,Husam S Younis,W Michael Flanagan,Arthur A Levin,Hanhua Huang,Georgios Karamanlidis

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ECH1 antibody [EPR15449(B)] - C-terminal

Flow Cytometry (Intracellular) - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (AB189255)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (AB189255)

Immunocytochemistry/ Immunofluorescence - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (AB189255)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (AB189255)

Immunoprecipitation - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (AB189255)

Western blot - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (AB189255)

Western blot - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (AB189255)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise