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Rabbit Recombinant Monoclonal ECH1 antibody. C-terminal. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples.

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Images

Western blot - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (AB189255), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (AB189255), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (AB189255), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (AB189255), expandable thumbnail
  • Immunoprecipitation - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (AB189255), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/60
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/10000 - 1/50000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

-

Tested
Tested

Species
Human
Dilution info
1/20
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species
Human
Dilution info
1/250 - 1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA.

Alternative names

ECH1

Recommended products

Rabbit Recombinant Monoclonal ECH1 antibody. C-terminal. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR15449(B)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ECH1 also known as Delta(35)-Delta(24)-dienoyl-CoA isomerase is a mitochondrial protein with a mass of approximately 31 kDa. It is expressed in a wide variety of tissues including high levels in the liver heart and muscle. This protein plays an important role in the beta-oxidation of unsaturated fatty acids inside mitochondria by catalyzing the conversion of 35-dienoyl-CoA to 24-dienoyl-CoA. Because of this function ECH1 contributes to the efficient metabolism of fatty acids.

Biological function summary

The ECH1 protein facilitates the breakdown and utilization of unsaturated fatty acids thereby providing energy for cellular processes. It acts independently and is not a part of a large protein complex. Mitochondria rely on ECH1 to handle specific intermediates in fatty acid metabolism which impacts how cells manage their energy resources. This makes ECH1 essential for maintaining energy homeostasis particularly during periods of increased metabolic demand.

Pathways

ECH1 is integral to the mitochondrial fatty acid beta-oxidation pathway. This pathway is important for converting fatty acids into acetyl-CoA units which then enter the Krebs cycle for further energy production. ECH1 interacts with other proteins like ACAA2 which contributes to the final step of fatty acid degradation. Alongside this it aligns with the peroxisomal biogenesis pathway influencing the balance between metabolic processes within mitochondria and peroxisomes.

Associated diseases and disorders

ECH1 activity impacts conditions such as metabolic syndrome and inherited disorders of mitochondrial fatty acid oxidation including Medium-chain acyl-CoA dehydrogenase (MCAD) deficiency. Changes in the function or expression of ECH1 can lead to accumulation of fatty acid intermediates causing cellular dysfunction. In diseases where energy balance is disrupted like metabolic syndrome ECH1's interaction with proteins like HADHA which assists in the final steps of beta-oxidation becomes significant for understanding and potentially targeting treatments.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255), expandable thumbnail

    Western blot - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)

    Lanes 1-4: Merged signal (red and green). Green - ab189255 observed at 36 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 observed at 50 kDa.

    ab189255 Anti-ECH1 antibody [EPR15449(B)] was shown to specifically react with ECH1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human ECH1 knockout HEK-293T cell line ab266748 (knockout cell lysate Human ECH1 knockout HEK-293T cell lysate ab257933) was used. Wild-type and ECH1 knockout samples were subjected to SDS-PAGE. ab189255 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255) at 1/1000 dilution

    Lane 1: Wild-type HEK293T cell lysate at 20 µg

    Lane 2: ECH1 knockout HEK293T cell lysate at 20 µg

    Lane 2: Western blot - Human ECH1 knockout HEK-293T cell line (Human ECH1 knockout HEK-293T cell line ab266748)

    Lane 3: A549 cell lysate at 20 µg

    Lane 4: U-2 OS cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 36 kDa, 80 kDa

    Observed band size: 100 kDa, 36 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)

    Immunhistochemical analysis of paraffin-embedded human endometrium tissue labeling ECH1 with Anti-alpha Sarcoglycan antibody [EPR14773] ab189254 at 1/500 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)

    Immunhistochemical analysis of paraffin-embedded human kidney tissue labeling ECH1 with Anti-alpha Sarcoglycan antibody [EPR14773] ab189254 at 1/500 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)

    Immunfluorescent analysis of 100% methanol(-20℃)-fixed MCF-7 cells labeling ECH1 with Anti-alpha Sarcoglycan antibody [EPR14773] ab189254 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with DAPI.

  • Immunoprecipitation - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255), expandable thumbnail

    Immunoprecipitation - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)

    Western blot analysis of ECH1 in human fetal liver lysate immunoprecipitated using Anti-alpha Sarcoglycan antibody [EPR14773] ab189254 at 1/50 dilution.

    Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

    All lanes: Immunoprecipitation - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)

    Predicted band size: 36 kDa

  • Western blot - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255), expandable thumbnail

    Western blot - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)

    All lanes: Western blot - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255) at 1/50000 dilution

    Lane 1: Human fetal liver lysate at 20 µg

    Lane 2: A549 cell lysate at 20 µg

    Lane 3: Jurkat cell lysate at 20 µg

    Lane 4: HeLa cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 36 kDa

    Observed band size: 33 kDa

  • Flow Cytometry (Intracellular) - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)

    Intracellular flow cytometric analysis of2% paraformaldehyde-fixed Jurkat cells labeling ECH1 with ab189255 at 1/20 dilution (red) compared to aRabbit monoclonal IgG isotype control (green), followed byGoat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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