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AB153732

Anti-ECHS1 antibody

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(1 Publication)

Rabbit Polyclonal ECHS1 antibody. Suitable for IHC-P, WB, ICC/IF, IP and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Enoyl-CoA hydratase, mitochondrial aa 50 to C-terminus.

View Alternative Names

mECH, mECH1, Enoyl-CoA hydratase 1, Short-chain enoyl-CoA hydratase, ECHS1, SCEH

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-ECHS1 antibody (AB153732)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ECHS1 antibody (AB153732)

Immunofluorescence analysis of HeLa cells fixed in ice-cold MeOH for 5 min labelling ECHS1 protein at mitochondria using ab153732 at a 1/500 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue).

Scale bar = 10 µm

Immunoprecipitation - Anti-ECHS1 antibody (AB153732)
  • IP

Supplier Data

Immunoprecipitation - Anti-ECHS1 antibody (AB153732)

Immunoprecipitation of ECHS1 protein from 293T whole cell extracts using 5 µg of ECHS1 antibody. Western blot analysis was performed using ECHS1 antibody .

All lanes:

Immunoprecipitation - Anti-ECHS1 antibody (ab153732) at 5 µg

Lanes 1 and 3:

293T whole cell extract

Lane 2:

293T whole cell extract with control IgG

Secondary

All lanes:

anti-rabbit IgG

Predicted band size: 31 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECHS1 antibody (AB153732)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECHS1 antibody (AB153732)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue stained for ECHS1 protein at mitochondria on mouse liver with ab153732 at a 1/500 dilution.

Antigen retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western blot - Anti-ECHS1 antibody (AB153732)
  • WB

Unknown

Western blot - Anti-ECHS1 antibody (AB153732)

12% SDS PAGE

All lanes:

Western blot - Anti-ECHS1 antibody (ab153732) at 1/3000 dilution

Lane 1:

NT2D1 whole cell lysate at 30 µg

Lane 2:

PC3 whole cell lysate at 30 µg

Lane 3:

U-87 MG whole cell lysate at 30 µg

Lane 4:

SK N SH whole cell lysate at 30 µg

Predicted band size: 31 kDa

false

Western blot - Anti-ECHS1 antibody (AB153732)
  • WB

Supplier Data

Western blot - Anti-ECHS1 antibody (AB153732)

Samples were separated by 12 % SDS.

All lanes:

Western blot - Anti-ECHS1 antibody (ab153732) at 1/500 dilution

Lane 1:

HeLa whole cell extract at 30 µg

Lane 2:

HepG2 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 31 kDa

false

Western blot - Anti-ECHS1 antibody (AB153732)
  • WB

Unknown

Western blot - Anti-ECHS1 antibody (AB153732)

12% SDS PAGE

All lanes:

Western blot - Anti-ECHS1 antibody (ab153732) at 1/1000 dilution

Lane 1:

Neuro2A whole cell lysate at 30 µg

Lane 2:

GL261 whole cell lysate at 30 µg

Lane 3:

C8D30 whole cell lysate at 30 µg

Predicted band size: 31 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF, IHC-P, IP

applications

Immunogen

Recombinant Fragment Protein within Human Enoyl-CoA hydratase, mitochondrial aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P30084

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ECHS1 Enoyl-CoA Hydratase mitochondrial also known as short-chain enoyl-CoA hydratase 1 is a mitochondrial enzyme involved in fatty acid metabolism. It has a molecular mass of approximately 30 kDa. This protein is expressed predominantly in tissues with high energy demands like heart and skeletal muscle. ECHS1 catalyzes the hydration of enoyl-CoA to 3-hydroxyacyl-CoA playing a significant role in the beta-oxidation pathway of fatty acid breakdown.
Biological function summary

ECHS1 functions as an important enzyme in the mitochondrial fatty acid degradation pathway. It participates as a component of the fatty acid beta-oxidation complex where it collaborates with other enzymes to facilitate energy production through lipids. ECHS1 ensures the conversion of unsaturated fatty acids into metabolites usable in subsequent steps of the energy cycle supporting cellular metabolism.

Pathways

ECHS1 lies within the beta-oxidation pathway of fatty acids important for the energy supply in mitochondria. This pathway directly links to the citric acid cycle (Krebs cycle) where acetyl-CoA produced through beta-oxidation gets further oxidized for energy generation. ECHS1 functions in concert with other enzymes such as Acyl-CoA dehydrogenases that precede its action in the pathway ensuring complete fatty acid degradation.

Defects in ECHS1 activity associate with rare metabolic disorders like short-chain enoyl-CoA hydratase deficiency. This deficiency leads to a build-up of toxic metabolites affecting the nervous system and muscle function. Moreover abnormalities in ECHS1 expression can relate to mitochondrial diseases impacting proteins such as Pyruvate dehydrogenase which further disrupts energy metabolism. Understanding ECHS1's role in these processes has important implications for diagnosing and developing treatments for metabolic conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Converts unsaturated trans-2-enoyl-CoA species ((2E)-enoyl-CoA) to the corresponding (3S)-3hydroxyacyl-CoA species through addition of a water molecule to the double bond (PubMed : 25125611, PubMed : 26251176). Catalyzes the hydration of medium- and short-chained fatty enoyl-CoA thioesters from 4 carbons long (C4) up to C16 (PubMed : 26251176). Has high substrate specificity for crotonyl-CoA ((2E)-butenoyl-CoA) and moderate specificity for acryloyl-CoA, 3-methylcrotonyl-CoA (3-methyl-(2E)-butenoyl-CoA) and methacrylyl-CoA ((2E)-2-methylpropenoyl-CoA) (PubMed : 26251176). Can bind tiglyl-CoA (2-methylcrotonoyl-CoA), but hydrates only a small amount of this substrate (PubMed : 26251176). Plays a key role in the beta-oxidation spiral of short- and medium-chain fatty acid oxidation (PubMed : 25125611, PubMed : 26251176). At a lower rate than the hydratase reaction, catalyzes the isomerase reaction of trans-3-enoyl-CoA species (such as (3E)-hexenoyl-CoA) to trans-2-enoyl-CoA species (such as (2E)-hexenoyl-CoA), which are subsequently hydrated to 3(S)-3-hydroxyacyl-CoA species (such as (3S)-hydroxyhexanoyl-CoA) (By similarity).
See full target information Enoyl-CoA hydratase, mitochondrial
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of medical genetics 52:691-8 PubMed26251176

2015

Clinical, biochemical and metabolic characterisation of a mild form of human short-chain enoyl-CoA hydratase deficiency: significance of increased N-acetyl-S-(2-carboxypropyl)cysteine excretion.

Applications

Unspecified application

Species

Unspecified reactive species

Kenichiro Yamada,Kaori Aiba,Yasuyuki Kitaura,Yusuke Kondo,Noriko Nomura,Yuji Nakamura,Daisuke Fukushi,Kei Murayama,Yoshiharu Shimomura,James Pitt,Seiji Yamaguchi,Kenji Yokochi,Nobuaki Wakamatsu
View all publications
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Product promise

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