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Rabbit Recombinant Monoclonal ECM antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECM Isoform 1 antibody [EPR25810-46] - BSA and Azide free (AB314894), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ECM Isoform 1 antibody [EPR25810-46] - BSA and Azide free (AB314894), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECM Isoform 1 antibody [EPR25810-46] - BSA and Azide free (AB314894), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECM Isoform 1 antibody [EPR25810-46] - BSA and Azide free (AB314894), expandable thumbnail
  • Western blot - Anti-ECM Isoform 1 antibody [EPR25810-46] - BSA and Azide free (AB314894), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFIPFlow Cyt (Intra)
Human
Tested
Tested
Tested
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Carrier protein for platelet (but not plasma) factor V/Va. Plays a role in the storage and stabilization of factor V in platelets. Upon release following platelet activation, may limit platelet and plasma factor Va-dependent thrombin generation. Ligand for integrin alpha-IIb/beta-3 and integrin alpha-V/beta-3 on activated platelets, and may function as an extracellular matrix or adhesive protein.

Alternative names

ECM, EMILIN4, GPIA*, MMRN, MMRN1, Multimerin-1, EMILIN-4, Elastin microfibril interface located protein 4, Endothelial cell multimerin, Elastin microfibril interfacer 4

Recommended products

Rabbit Recombinant Monoclonal ECM antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR25810-46
Purification technique
Affinity purification Protein A
Specificity

This antibody is specifically recognized ECM Isoform 1.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab314894 is the carrier-free version of Anti-ECM Isoform 1 antibody [EPR25810-46] ab314893.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ECM Isoform 1 also known as Extracellular Matrix Protein Isoform 1 is a structural component of the extracellular matrix (ECM). It has a mass of approximately 90 kDa. This protein is mainly expressed in connective tissue playing a role in maintaining structural integrity of tissues by providing a scaffold for cellular attachment. The ECM in which ECM Isoform 1 participates is important for tissue repair and cellular signaling making it essential for various physiological processes.

Biological function summary

ECM Isoform 1 contributes to tissue homeostasis and repair processes. It is part of a complex matrix that interacts with integrins and other cell surface receptors facilitating cell adhesion and migration. The protein also influences cell differentiation and proliferation by interacting with growth factors. These interactions help modulate cellular responses required for tissue repair immune responses and development.

Pathways

ECM Isoform 1 plays a role in the integrin signaling and wound healing pathways. It interacts with integrin receptors mediating signals that regulate cell adhesion and migration. These pathways are important for cellular responses to injury and inflammation. ECM Isoform 1 also connects with proteins like collagen and fibronectin which further supports structural integrity and interactions within the ECM.

Associated diseases and disorders

ECM Isoform 1 has been implicated in fibrosis and cancer. Alterations in its expression or structure can contribute to the development of fibrotic tissues where excessive ECM deposition occurs. The protein also connects with matrix metalloproteinases (MMPs) enzymes that degrade ECM components influencing cancer metastasis by affecting the tumor microenvironment and facilitating the invasion of cancer cells. Understanding its interactions and modifications in these conditions may provide insights into therapeutic strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECM Isoform 1 antibody [EPR25810-46] - BSA and Azide free (ab314894), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECM Isoform 1 antibody [EPR25810-46] - BSA and Azide free (ab314894)

    This data was developed using Anti-ECM Isoform 1 antibody [EPR25810-46] ab314893, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling ECM Isoform 1 with Anti-ECM Isoform 1 antibody [EPR25810-46] ab314893 at 1/5000 (0.104 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on platelets in human spleen. The section was incubated with Anti-ECM Isoform 1 antibody [EPR25810-46] ab314893 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunocytochemistry/ Immunofluorescence - Anti-ECM Isoform 1 antibody [EPR25810-46] - BSA and Azide free (ab314894), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ECM Isoform 1 antibody [EPR25810-46] - BSA and Azide free (ab314894)

    This data was developed using Anti-ECM Isoform 1 antibody [EPR25810-46] ab314893, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HUVEC (human umbilical vein endothelial cell) cells labelling ECM Isoform 1 with Anti-ECM Isoform 1 antibody [EPR25810-46] ab314893 at 1/100 (1.044 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/mL) dilution (Green). Confocal image showing cytoplasmic staining in HUVEC cell line. Negative control: A549. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECM Isoform 1 antibody [EPR25810-46] - BSA and Azide free (ab314894), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECM Isoform 1 antibody [EPR25810-46] - BSA and Azide free (ab314894)

    This data was developed using Anti-ECM Isoform 1 antibody [EPR25810-46] ab314893, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling ECM Isoform 1 with Anti-ECM Isoform 1 antibody [EPR25810-46] ab314893 at 1/5000 (0.104 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on human cardiac muscle. The section was incubated with Anti-ECM Isoform 1 antibody [EPR25810-46] ab314893 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECM Isoform 1 antibody [EPR25810-46] - BSA and Azide free (ab314894), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECM Isoform 1 antibody [EPR25810-46] - BSA and Azide free (ab314894)

    This data was developed using Anti-ECM Isoform 1 antibody [EPR25810-46] ab314893, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling ECM Isoform 1 with Anti-ECM Isoform 1 antibody [EPR25810-46] ab314893 at 1/5000 (0.104 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on endothelial cells and platelets in human liver. The section was incubated with Anti-ECM Isoform 1 antibody [EPR25810-46] ab314893 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-ECM Isoform 1 antibody [EPR25810-46] - BSA and Azide free (ab314894), expandable thumbnail

    Western blot - Anti-ECM Isoform 1 antibody [EPR25810-46] - BSA and Azide free (ab314894)

    This data was developed using Anti-ECM Isoform 1 antibody [EPR25810-46] ab314893, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Negative control: A549

    The molecular weight observed is consistent with what has been described in the literature (PMID: 33179420, 19132231).

    Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-ECM Isoform 1 antibody [EPR25810-46] (Anti-ECM Isoform 1 antibody [EPR25810-46] ab314893) at 1/1000 dilution

    Lane 1: HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg

    Lane 2: A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 186 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-ECM Isoform 1 antibody [EPR25810-46] - BSA and Azide free (ab314894), expandable thumbnail

    Western blot - Anti-ECM Isoform 1 antibody [EPR25810-46] - BSA and Azide free (ab314894)

    This data was developed using Anti-ECM Isoform 1 antibody [EPR25810-46] ab314893, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Low expression: plasma

    The molecular weight observed is consistent with what has been described in the literature (PMID: 33179420, 19132231).

    All lanes: Western blot - Anti-ECM Isoform 1 antibody [EPR25810-46] (Anti-ECM Isoform 1 antibody [EPR25810-46] ab314893) at 1/1000 dilution

    Lane 1: Human serum at 20 µg

    Lane 2: Human plasma at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 186 kDa

    Exposure time: 59s

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Product protocols

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