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AB28666

Anti-ECM29 antibody

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(4 Publications)

Rabbit Polyclonal ECM29 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human ECPAS aa 1800 to C-terminus.

View Alternative Names

ECM29, KIAA0368, ECPAS, Proteasome adapter and scaffold protein ECM29, Ecm29 proteasome adapter and scaffold, Proteasome-associated protein ECM29 homolog

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-ECM29 antibody (AB28666)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ECM29 antibody (AB28666)

Immunofluorescent staining of Ecm29 in HeLa cells using ab28666 (1/200 dilution).

Immunocytochemistry/ Immunofluorescence - Anti-ECM29 antibody (AB28666)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ECM29 antibody (AB28666)

Immunofluorescence analysis of HeLa cells labelling ECM29 with ab28666 at 1/250 dilution. Cells were fixed with 4% paraformaldehyde for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1 : 2000 dilution for 45 minutes at room temperature was used as the secondary antibody (green) (a). F-actin (was stained with Rhodamine Phalloidin (red) (c) and Nuclear DNA was labelled with DAPI (blue) (b). (d) represents the merged image showing cytoplasmic localization. (e) shows the no primary antibody control. The images were captured at 60X magnification.

Western blot - Anti-ECM29 antibody (AB28666)
  • WB

Unknown

Western blot - Anti-ECM29 antibody (AB28666)

Detection of ECM29 in HeLa cells.

All lanes:

Western blot - Anti-ECM29 antibody (ab28666)

All lanes:

HeLa cell extract

Predicted band size: 205 kDa

Observed band size: 205 kDa

false

Western blot - Anti-ECM29 antibody (AB28666)
  • WB

Unknown

Western blot - Anti-ECM29 antibody (AB28666)

Additional bands of 70 and 160 kDa observed might correspond to interacting partners of dynamin intermediate chain and kinesin intermediate chain respectively.

All lanes:

Western blot - Anti-ECM29 antibody (ab28666) at 1/250 dilution

Lane 1:

HeLa whole cell lysate

Lane 2:

K-562 whole cell lysate

Lane 3:

NTERA-2 cl.D1 whole cell lysate

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody at 1/2500 dilution

Predicted band size: 205 kDa

Observed band size: 204 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human ECPAS aa 1800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q5VYK3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
Preservative: 0.05% Sodium azide Constituents: Whole serum
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ECM29 also known as proteasome-associated protein ECM29 homolog is a multi-domain protein with a molecular mass of approximately 209 kDa. The protein acts as an adaptor that links molecular chaperones to the proteasome. Highly expressed in tissues with high proteasome activity ECM29 mainly locates in the cytosol. The structure of ECM29 suggests it interacts with the proteasomal regulatory particle altering its conformation and functional state.
Biological function summary

ECM29 impacts protein degradation by stabilizing the interaction between the 19S regulatory particle and the 26S proteasome core complex. It plays a role in ensuring the correct assembly of the proteasome which is essential for the ubiquitin-proteasome system. This system is responsible for the majority of protein turnover in the cell impacting various cell cycle processes. ECM29's association with other proteasome components like Rpn1 and Rpn2 signifies its role in protein homeostasis.

Pathways

ECM29 integrates into the ubiquitin-proteasome pathway and cell cycle regulation mechanisms. It coordinates with proteins such as ubiquitin and Rpn10 to manage protein degradation. Its involvement in NAD(P)H pathway regulation has been noticed where it potentially influences cellular responses to stress by modulating proteasome activity under different conditions.

Variations in ECM29 expression and regulation implicate it in neurodegenerative diseases such as Alzheimer's. Aberrant proteasome function connected to ECM29 can disrupt protein clearance contributing to protein aggregation observed in Alzheimer's. Additionally ECM29 interacts with SYT7 influencing synaptic function and potentially impacting neuropathologies. Understanding ECM29's function provides deeper insight into therapeutic targets for managing proteasome-related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adapter/scaffolding protein that binds to the 26S proteasome, motor proteins and other compartment specific proteins. May couple the proteasome to different compartments including endosome, endoplasmic reticulum and centrosome. May play a role in ERAD and other enhanced proteolysis (PubMed : 15496406). Promotes proteasome dissociation under oxidative stress (By similarity).
See full target information ECPAS
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:650817 PubMed34055780

2021

Ecm29-Dependent Proteasome Localization Regulates Cytoskeleton Remodeling at the Immune Synapse.

Applications

Unspecified application

Species

Unspecified reactive species

Jorge Ibañez-Vega,Felipe Del Valle,Juan José Sáez,Fanny Guzman,Jheimmy Diaz,Andrea Soza,María Isabel Yuseff

The Journal of cell biology 219: PubMed31910261

2020

Ecm29-mediated proteasomal distribution modulates excitatory GABA responses in the developing brain.

Applications

Unspecified application

Species

Unspecified reactive species

Min Lee,Yen-Chen Liu,Chen Chen,Chi-Huan Lu,Shao-Tzu Lu,Tzyy-Nan Huang,Meng-Tsung Hsu,Yi-Ping Hsueh,Pei-Lin Cheng

The EMBO journal 37: PubMed29669860

2018

Thermal proteome profiling of breast cancer cells reveals proteasomal activation by CDK4/6 inhibitor palbociclib.

Applications

Unspecified application

Species

Unspecified reactive species

Teemu P Miettinen,Julien Peltier,Anetta Härtlova,Marek Gierliński,Valerie M Jansen,Matthias Trost,Mikael Björklund

Experimental and therapeutic medicine 15:3189-3196 PubMed29545834

2018

Human fibroblast growth factor-21 serves as a predictor and prognostic factor in patients with hepatitis B cirrhosis combined with adrenal insufficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Zhang,Junhong Li,Junwei Ma,Hongxin Wang,Yin Yi
View all publications
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Product promise

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