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AB211475

Anti-Eco1 antibody - C-terminal

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Rabbit Polyclonal Eco1 antibody. C-terminal. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human ESCO1 aa 650-700 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

EFO1, KIAA1911, ESCO1, N-acetyltransferase ESCO1, CTF7 homolog 1, Establishment factor-like protein 1, Establishment of cohesion 1 homolog 1, EFO1p, hEFO1, ECO1 homolog 1, ESO1 homolog 1

4 Images
Western blot - Anti-Eco1 antibody - C-terminal (AB211475)
  • WB

Supplier Data

Western blot - Anti-Eco1 antibody - C-terminal (AB211475)

All lanes:

Western blot - Anti-Eco1 antibody - C-terminal (ab211475) at 1/1000 dilution

All lanes:

Mouse spleen tissue lysate at 35 µg

Predicted band size: 95 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eco1 antibody - C-terminal (AB211475)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eco1 antibody - C-terminal (AB211475)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labelling Eco1 with ab211475 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eco1 antibody - C-terminal (AB211475)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eco1 antibody - C-terminal (AB211475)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue labelling Eco1 with ab211475 at 1/100 dilution.

Western blot - Anti-Eco1 antibody - C-terminal (AB211475)
  • WB

Supplier Data

Western blot - Anti-Eco1 antibody - C-terminal (AB211475)

All lanes:

Western blot - Anti-Eco1 antibody - C-terminal (ab211475) at 1/1000 dilution

All lanes:

293 cell lysate at 35 µg

Predicted band size: 95 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human ESCO1 aa 650-700 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q5FWF5

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: 99% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Eco1 also known as Esco1 or Establishment of Sister Chromatid Cohesion N-Acetyltransferase 1 is an important enzyme in the establishment of sister chromatid cohesion during cell division. It weighs approximately 84 kDa. Eco1 primarily expresses in the nucleus of eukaryotic cells where it functions by acetylating the Smc3 subunit of the cohesin complex. This acetylation process is essential for the cohesin's capability to hold the sister chromatids together until they are ready to be separated during mitosis.
Biological function summary

Eco1 ensures chromatid cohesion which is important for accurate chromosome segregation. As a part of the cohesin complex Eco1 collaborates with other proteins like Smc1 Smc3 and Scc1 to maintain proper structure and function throughout the cell cycle. By acetylating Smc3 Eco1 prevents premature separation of sister chromatids contributing to the integrity of genetic information passed on to daughter cells. Furthermore Eco1's role directly influences DNA repair and replication processes affirming its importance in maintaining genomic stability.

Pathways

Eco1 participates in cohesion establishment and DNA damage response pathways. The protein plays a part in the cohesin complex's regulation during the cell cycle specifically impacting the cohesion establishment pathway. In response to DNA damage Eco1's acetylation activity is pivotal in maintaining chromosome stability. It interacts with other pathway-associated proteins such as Plk1 which modulates the timing of cohesin disassembly in mitosis. Eco1's function ensures precise chromatid allocation to daughter cells reducing the chances of aneuploidy and maintaining cellular homeostasis.

Malfunctions of Eco1 contribute to conditions like Roberts syndrome and certain cancers. Genetic mutations or deficiencies in Eco1 have been linked to Roberts syndrome characterized by growth retardation and limb malformations due to cohesion defects. Additionally due to its role in genomic stability disrupted Eco1 activity risks increased susceptibility to tumorigenesis. The protein interacts with other cohesion-related proteins such as Esco2 which is also implicated in similar pathologies. Understanding Eco1’s functions and affiliations helps clarify its implications in these conditions and aids in potential therapeutic target development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acetyltransferase required for the establishment of sister chromatid cohesion (PubMed : 15958495, PubMed : 18614053). Couples the processes of cohesion and DNA replication to ensure that only sister chromatids become paired together. In contrast to the structural cohesins, the deposition and establishment factors are required only during S phase. Acts by mediating the acetylation of cohesin component SMC3 (PubMed : 18614053).
See full target information ESCO1
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Product promise

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