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AB21288

Anti-ECSIT antibody

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(12 Publications)

Rabbit Polyclonal ECSIT antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human ECSIT.

View Alternative Names

Protein SITPEC, ECSIT

1 Images
Western blot - Anti-ECSIT antibody (AB21288)
  • WB

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Western blot - Anti-ECSIT antibody (AB21288)

Lane 1:

Western blot - Anti-ECSIT antibody (ab21288) at 0.5 µg/mL

Lane 2:

Western blot - Anti-ECSIT antibody (ab21288) at 1 µg/mL

Lane 3:

Western blot - Anti-ECSIT antibody (ab21288) at 2 µg/mL

All lanes:

human heart lysate

Predicted band size: 49 kDa

Observed band size: 42 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

IP, ICC/IF, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human ECSIT. The exact immunogen used to generate this antibody is proprietary information.

Q9BQ95

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Ion exchange chromatography.
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ECSIT also known as Evolutionarily Conserved Signaling Intermediate in Toll pathways is a mitochondrial protein involved in various cellular processes. It has a molecular mass of approximately 52 kDa and is prominently expressed in tissues with high metabolic activity such as the heart and skeletal muscles. ECSIT interacts with the mitochondrial membrane and is integral for mitochondrial function and biogenesis. Understanding the expression and function of ECSIT can provide insight into its role in cellular energy homeostasis.
Biological function summary

ECSIT influences several important cellular processes through its participation in the assembly of the mitochondrial complex I. It is part of a protein complex that includes NDUFAF1 and acts as a scaffold protein facilitating the proper assembly of complex I. ECSIT involvement is important for the maintenance of cellular respiration and energy production. Its role in complex I assembly highlights its importance in regulating mitochondrial activity and function.

Pathways

ECSIT acts in both mitochondrial biogenesis and the toll-like receptor (TLR) signaling pathway. In the TLR pathway ECSIT mediates interactions between TRAF6 and TAK1 contributing to the regulation of immune responses. It is an essential component linking mitochondrial function with immune signaling demonstrating its multi-functional capability. These pathways highlight the intricate network where ECSIT collaborates with proteins like TRAF6 and TAK1 to ensure proper immune response and energy metabolism.

ECSIT has associations with leigh syndrome and aggressive lung cancer. Its dysfunction in mitochondrial biogenesis can contribute to Leigh syndrome a severe neurological disorder. In lung cancer ECSIT involvement in TLR signaling affects cell proliferation and survival making it a protein of interest in cancer research. ECSIT's role in these conditions can involve interactions with mitochondrial proteins and signaling molecules like TRAF6 further illustrating its impact on human health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adapter protein that plays a role in different signaling pathways including TLRs and IL-1 pathways or innate antiviral induction signaling. Plays a role in the activation of NF-kappa-B by forming a signal complex with TRAF6 and TAK1/MAP3K7 to activate TAK1/MAP3K7 leading to activation of IKKs (PubMed : 25355951, PubMed : 31281713). Once ubiquitinated, interacts with the dissociated RELA and NFKB1 proteins and translocates to the nucleus where it induces NF-kappa-B-dependent gene expression (PubMed : 25355951). Plays a role in innate antiviral immune response by bridging the pattern recognition receptors RIGI and MDA5/IFIT1 to the MAVS complex at the mitochondrion (PubMed : 25228397). Promotes proteolytic activation of MAP3K1. Involved in the BMP signaling pathway. Required for normal embryonic development (By similarity).. As part of the MCIA complex, involved in the assembly of the mitochondrial complex I.
See full target information ECSIT
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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadw4153 PubMed41061082

2025

TRAF6 integrates innate immune signals to regulate glucose homeostasis via Parkin-dependent and Parkin-independent mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Levi-D'Ancona,Emily M Walker,Jie Zhu,Yamei Deng,Vaibhav Sidarala,Ava M Stendahl,Emma C Reck,Belle A Henry-Kanarek,Anne C Lietzke,Biaoxin Chai,Mabelle B Pasmooij,Dre L Hubers,Venkatesha Basrur,Sankar Ghosh,Linsey Stiles,Alexey I Nesvizhskii,Orian S Shirihai,Scott A Soleimanpour

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2414358 PubMed39746855

2025

ECSIT-X4 is Required for Preventing Pressure Overload-Induced Cardiac Hypertrophy via Regulating Mitochondrial STAT3.

Applications

Unspecified application

Species

Unspecified reactive species

Xia Lu,Tingting Tong,Haoliang Sun,Yi Chen,Yongfeng Shao,Pengxi Shi,Linli Que,Li Liu,Guoqing Zhu,Qi Chen,Chuanfu Li,Jiantao Li,Shuo Yang,Yuehua Li

Cardiovascular research 119:2213-2229 PubMed37395010

2023

Tissue-specific differences in the assembly of mitochondrial Complex I are revealed by a novel ENU mutation in ECSIT.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Nicol,Sara Falcone,Andrew Blease,Pratik Vikhe,Gabriele Civiletto,Saleh Salman Omairi,Carlo Viscomi,Ketan Patel,Paul K Potter

Frontiers in endocrinology 14:1110369 PubMed37152948

2023

ECSIT is essential for RANKL-induced stimulation of mitochondria in osteoclasts and a target for the anti-osteoclastogenic effects of estrogens.

Applications

Unspecified application

Species

Unspecified reactive species

Adriana Marques-Carvalho,Vilma A Sardão,Ha-Neui Kim,Maria Almeida

iScience 24:103047 PubMed34553133

2021

Synthetic VSMCs induce BBB disruption mediated by MYPT1 in ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Hailan Meng,Lizhen Fan,Cun-Jin Zhang,Liwen Zhu,Pinyi Liu,Jian Chen,Xinyu Bao,Zhijun Pu,Min-Sheng Zhu,Yun Xu

Journal of immunology (Baltimore, Md. : 1950) 207:1627-1640 PubMed34433619

2021

Metabolic Adaptation of Macrophages as Mechanism of Defense against Crystalline Silica.

Applications

Unspecified application

Species

Unspecified reactive species

Antonella Marrocco,Krystin Frawley,Linda L Pearce,James Peterson,James P O'Brien,Steven J Mullett,Stacy G Wendell,Claudette M St Croix,Steven E Mischler,Luis A Ortiz

Scientific reports 10:11183 PubMed32636430

2020

MTFMT deficiency correlates with reduced mitochondrial integrity and enhanced host susceptibility to intracellular infection.

Applications

Unspecified application

Species

Unspecified reactive species

Jung-Hwa Seo,Cheol-Sang Hwang,Joo-Yeon Yoo

Frontiers in immunology 10:2203 PubMed31620128

2019

CRBN Is a Negative Regulator of Bactericidal Activity and Autophagy Activation Through Inhibiting the Ubiquitination of ECSIT and BECN1.

Applications

Unspecified application

Species

Unspecified reactive species

Mi-Jeong Kim,Yoon Min,Jae-Hyuck Shim,Eunyoung Chun,Ki-Young Lee

Autophagy 14:1347-1358 PubMed29929436

2018

Inhibition of TRAF6 ubiquitin-ligase activity by PRDX1 leads to inhibition of NFKB activation and autophagy activation.

Applications

Unspecified application

Species

Unspecified reactive species

Yoon Min,Mi-Jeong Kim,Sena Lee,Eunyoung Chun,Ki-Young Lee

Molecular and cellular biology 34:847-61 PubMed24344204

2013

TIMMDC1/C3orf1 functions as a membrane-embedded mitochondrial complex I assembly factor through association with the MCIA complex.

Applications

Unspecified application

Species

Unspecified reactive species

Virginia Guarani,Joao Paulo,Bo Zhai,Edward L Huttlin,Steven P Gygi,J Wade Harper
View all publications
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