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AB86604

Anti-ECT2 antibody

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(1 Publication)

Rabbit Polyclonal ECT2 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Protein ECT2, Epithelial cell-transforming sequence 2 oncogene, ECT2

1 Images
Western blot - Anti-ECT2 antibody (AB86604)
  • WB

Unknown

Western blot - Anti-ECT2 antibody (AB86604)

Chemiluminescence with an exposure time of 3 minutes

All lanes:

Western blot - Anti-ECT2 antibody (ab86604) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HEK293T whole cell lysate at 50 µg

Lane 3:

NIH 3T3 whole cell lysate at 50 µg

Predicted band size: 104 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ECT2 also known as epithelial cell transforming sequence 2 is a guanine nucleotide exchange factor. It has a molecular mass of about 103 kDa. ECT2 is mostly expressed in proliferating cells including tumor cells and plays a role in cell cycle regulation. In its mechanical function ECT2 activates Rho family GTPases especially RhoA by facilitating the exchange of GDP for GTP. This activation is important for the regulation of cytoskeletal dynamics and cell division.
Biological function summary

The regulation of cytoskeletal rearrangement and cell cycle progression involves ECT2. It is an essential part of the mitotic spindle assembly complex. ECT2 interacts with several cellular components to coordinate proper cytokinesis. This activity ensures that the cells divide correctly maintaining proper cell number and tissue homeostasis. ECT2's interaction with other proteins also determines its role in controlling cell shape and movement contributing further to cell cycle events.

Pathways

ECT2 importantly participates in the RhoA signaling pathway which regulates the actin cytoskeleton. The proper function of this pathway is essential for cytokinesis and cell migration. ECT2's activity intersects with proteins like ROCK1 and ROCK2 which act downstream of RhoA to influence cell structure and integrity. In addition to RhoA signaling ECT2 is also involved in the Hippo pathway contributing to cell proliferation controls and tissue growth.

Scientists have found ECT2's involvement in cancer specifically in glioblastoma and breast cancer. Its overexpression in tumors correlates with increased cell proliferation and metastatic potential. In cancer progression ECT2 works with proteins like RhoA and YAP1 in Hippo signaling to influence cell growth and survival making it a target of interest in cancer research and therapeutic development. The aberrant regulation of ECT2 and its pathway partners can lead to uncontrolled cell division and tumor growth.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the equatorial region. Plays a role in the control of mitotic spindle assembly; regulates the activation of CDC42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in the regulation of epithelial cell polarity; participates in the formation of epithelial tight junctions in a polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite outgrowth. Inhibits phenobarbital (PB)-induced NR1I3 nuclear translocation. Stimulates the activity of RAC1 through its association with the oncogenic PARD6A-PRKCI complex in cancer cells, thereby acting to coordinately drive tumor cell proliferation and invasion. Also stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.
See full target information ECT2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 13:158 PubMed35177591

2022

Targeting CCL2-CCR4 axis suppress cell migration of head and neck squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zihang Ling,Wei Li,Jiaqi Hu,Yuanyuan Li,Miao Deng,Siyuan Zhang,Xianyue Ren,Tong Wu,Juan Xia,Bin Cheng,Xiaoan Tao
View all publications
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