Anti-ECT2 antibody [ECO311E]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECT2 antibody [ECO311E] (AB324657)

Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling ECT2 with ab324657 at 1/100 (9.37 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human cervical cancer.
The primary antibody was incubated for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECT2 antibody [ECO311E] (AB324657)

Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling ECT2 with ab324657 at 1/100 (9.37 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human colon cancer.
The primary antibody was incubated for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• I-ELISA

Supplier Data

Indirect ELISA - Anti-ECT2 antibody [ECO311E] (AB324657)

Indirect ELISA analysis of ab324657 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1 : 1000 dilution dilution.

Antigen : Human ECT2 protein.

Antigen concentration : 1000 ng/ml

• WB

Supplier Data

Western blot - Anti-ECT2 antibody [ECO311E] (AB324657)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : THP-1 and IMR-90.

The bands beneath the target band (103 kDa) are likely to be degraded target fragments.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ECT2 antibody [ECO311E] (ab324657) at 1/1000 dilution

Lane 1:

KATO III (human stomach spherical) whole cell lysate at 20 µg

Lane 2:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

Lane 4:

IMR-90 (human lung fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

Observed band size: 103 kDa,36 kDa

true

Exposure time: 114s

• WB

Supplier Data

Western blot - Anti-ECT2 antibody [ECO311E] (AB324657)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ECT2 antibody [ECO311E] (ab324657) at 1/1000 dilution

Lane 1:

HeLa transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HeLa transfected with siRNA specifically targeting ECT2 whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

Observed band size: 103 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-ECT2 antibody [ECO311E] (AB324657)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The bands beneath the target band (103 kDa) are likely to be degraded target fragments.

This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-ECT2 antibody [ECO311E] (ab324657) at 1/1000 dilution

Lane 1:

LoVo (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

SW620 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/1000 dilution

Observed band size: 103 kDa

true

Exposure time: 114s

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECT2 antibody [ECO311E] (AB324657)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling ECT2 with ab324657 at 1/100 (9.37 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on human pancreas.
The primary antibody was incubated for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins