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AB125233

Anti-EDA antibody

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(2 Publications)

Rabbit Polyclonal EDA antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human EDA.

View Alternative Names

ED1, EDA2, EDA, Ectodysplasin-A, Ectodermal dysplasia protein, EDA protein

1 Images
Western blot - Anti-EDA antibody (AB125233)
  • WB

Unknown

Western blot - Anti-EDA antibody (AB125233)

All lanes:

Western blot - Anti-EDA antibody (ab125233) at 1 µg/mL

All lanes:

SW620 Cell Lysate

Predicted band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human EDA. The exact immunogen used to generate this antibody is proprietary information.

Q92838

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate) Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EDA also known as Ectodysplasin A is a protein encoded by the EDA gene. It is a member of the tumor necrosis factor (TNF) family and is expressed in epithelial cells. The molecular mass of EDA is approximately 47 kDa. EDA plays a mechanical role in signaling processes that are important for developmental pathways. Its expression is observed in tissues like skin hair follicles and some glandular cells where it contributes to cellular communication.
Biological function summary

Ectodysplasin A influences the development and differentiation of epithelial tissues. It plays a role in the formation of hair shafts sweat glands and other ectodermal structures. EDA operates as part of a signaling complex influencing cell fate decisions through binding to its receptor EDA receptor (EDAR). This interaction initiates processes for the development and maintenance of these structures impacting cell proliferation and differentiation.

Pathways

Not only does EDA interact in the TNF signaling pathway it also engages in the NF-kB pathway. The NF-kB pathway initiates transcription of genes responsible for cell survival and inflammatory responses. EDA interaction with EDAR also involves signaling pathways intersecting with NF-kB leading to activation of downstream proteins like TRAF6 and NEMO. These proteins orchestrate cell proliferation and apoptotic regulation confirming EDA's role in strategic developmental and signaling pathways.

Abnormal function or mutations in EDA link to conditions such as X-linked hypohidrotic ectodermal dysplasia (XLHED) and immune system irregularities. XLHED occurs due to EDA gene mutations leading to missing or improperly developed ectodermal structures like hair and teeth. EDA's association with XLHED connects it to EDAR and down to other proteins such as NF-kB indicating the broader genetic mutations' impact on downstream signaling which results in specific phenotype alterations seen in the disorder.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytokine which is involved in epithelial-mesenchymal signaling during morphogenesis of ectodermal organs. Functions as a ligand activating the DEATH-domain containing receptors EDAR and EDA2R (PubMed : 11039935, PubMed : 27144394, PubMed : 34582123, PubMed : 8696334). May also play a role in cell adhesion (By similarity).. Isoform 1. Binds only to the receptor EDAR, while isoform 3 binds exclusively to the receptor EDA2R.. Isoform 3. Binds only to the receptor EDA2R.
See full target information EDA
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 8:e2103079 PubMed34569165

2021

Sweat Gland Organoids Originating from Reprogrammed Epidermal Keratinocytes Functionally Recapitulated Damaged Skin.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Sun,Jiangbing Xiang,Runkai Chen,Zhijun Geng,Lintao Wang,Yiqiong Liu,Shuaifei Ji,Huating Chen,Yan Li,Cuiping Zhang,Peng Liu,Tao Yue,Lei Dong,Xiaobing Fu

Molecular medicine reports 17:4688-4694 PubMed29344666

2018

Let-7b regulates alpaca hair growth by downregulating ectodysplasin A.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Liu,Shu Niu,Xiao-Rui Cao,Jia-Qi Cheng,Shu-Yuan Gao,Xiu-Ju Yu,Hai-Dong Wang,Chang-Sheng Dong,Xiao-Yan He
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Product promise

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