JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB245684

Anti-EDAR antibody [EDAR12]

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Monoclonal EDAR antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Protein within Mouse Edar.

View Alternative Names

DL, EDAR, Tumor necrosis factor receptor superfamily member EDAR, Anhidrotic ectodysplasin receptor 1, Downless homolog, EDA-A1 receptor, Ectodermal dysplasia receptor, Ectodysplasin-A receptor

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-EDAR antibody [EDAR12] (AB245684)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-EDAR antibody [EDAR12] (AB245684)

PFA fixed, 0.15% Triton permeabilized A549 (Human lung carcinoma cell line) cells labeling EDAR using ab245684 at 10 μg/ml, 1 hr (green) followed by Alexa Fluor® 488 secondary antibody (2 μg/ml), in ICC/IF. Nuclear probe is visualized in blue (DAPI).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EDAR12

Isotype

IgG

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Protein within Mouse Edar. The exact immunogen used to generate this antibody is proprietary information.

Q9R187

Epitope

CRD1 of the extracellular domain of EDAR.

Specificity

EDAR12 recognises and binds to CRD1 of the extracellular domain of mouse EDAR. EDAR3 cross-reacts with EDAR derived from human, dog, rat and chicken when EDAR is fused to the glycosylphosphatidylinositol anchor of TRAILR3. EDAR is the receptor for the TNF family ligand EDA1, which is a type II transmembrane protein possessing a collagen-like domain and a C-terminal TNF-homology domain. EDAR is important for the correct development of skin appendages including hair, teeth and eccrine sweat glands. LoF mutations in the Eda gene is known to cause XLHED (X-linked hypohidrotic ectodermal dysplasia), and results in abnormal development.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.02% Proclin 300 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ectodysplasin A receptor also known as EDAR is a transmembrane protein involved in various developmental processes. It has a molecular mass of approximately 63 kDa. You can find EDAR expression in tissues such as the skin hair follicles and teeth. As a member of the tumor necrosis factor receptor (TNFR) family EDAR plays an important mechanical role by binding ectodysplasin A ligand (EDA) which initiates downstream signals important for developmental pathways.
Biological function summary

The interaction between EDAR and its ligand EDA influences morphogenesis of ectodermal-derived structures. This receptor-ligand binding is integral to the EDAR/NF-kappaB signaling complex. It affects processes such as the formation of hair follicles sweat glands and teeth. The receptor facilitates cell communication necessary for the proper development of these structures ensuring the correct activation of related signaling pathways.

Pathways

EDAR functions within the NF-kappaB and Wnt signaling pathways. These pathways regulate cellular differentiation and proliferation. The receptor collaborates with adapter proteins such as EDARADD to effectively transmit signals downstream. Furthermore it shares significant relationships with other TNF receptor family members highlighting the importance of signaling specificity and coordination within these biological pathways.

Mutations in the EDAR gene associate with ectodermal dysplasia a genetic disorder affecting hair teeth and sweat glands. Additionally EDAR influences the development of hypohidrotic ectodermal dysplasia (HED). The receptor's link with EDA and other TNFR family members highlights the complex genetic interactions contributing to these conditions. Understanding these relationships provides insights into targeted therapeutic strategies addressing developmental abnormalities.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Receptor for EDA isoform A1, but not for EDA isoform A2. Mediates the activation of NF-kappa-B and JNK. May promote caspase-independent cell death.
See full target information EDAR
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

CytoJournal 22:24 PubMed40260072

2025

Interactions of tumor necrosis factor receptor-associated factor 4 and pyruvate kinase muscle isoform 2 promote malignant behavior and aerobic glycolysis in colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tongming Liu,Shihong Zhu,Jiawei Sun,Yuanyuan Ma
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB235106

Anti-EDAR antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDAR antibody (AB235106)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB125233

Anti-EDA antibody

Western blot - Anti-EDA antibody (AB125233)

  • 0
  • 0
Primary Antibodies

AB187027

Anti-RhoA antibody [EPR18134]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-RhoA antibody [EPR18134] (AB187027)

  • 4
  • 1
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB285061

Anti-TACI antibody [EPR23045-70] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-TACI antibody [EPR23045-70] - BSA and Azide free (Detector) (AB285061)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com