AB168851

Anti-EDC3 antibody

Name: Anti-EDC3 antibody (ab168851) | Abcam
Brand: Abcam Limited
SKU: AB168851
Price: 535 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal EDC3 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human EDC3 aa 1-100.

View Alternative Names

LSM16, YJDC, YJEFN2, PP844, EDC3, Enhancer of mRNA-decapping protein 3, LSM16 homolog, YjeF N-terminal domain-containing protein 2, YjeF domain-containing protein 1, YjeF_N2, hYjeF_N2

2 Images

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Immunoprecipitation - Anti-EDC3 antibody (AB168851)

Detection of EDC3 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168851 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
Detection : Chemiluminescence with an exposure time of 10 seconds

All lanes:

Immunoprecipitation - Anti-EDC3 antibody (ab168851)

Predicted band size: 56 kDa

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Western blot - Anti-EDC3 antibody (AB168851)

All lanes:

Western blot - Anti-EDC3 antibody (ab168851) at 0.1 µg

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK-1 whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 56 kDa

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Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human EDC3 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q96F86

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab168851 was affinity purified using an epitope specific to EDC3 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The EDC3 protein also known as Enhancer of mRNA Decapping 3 serves an essential role in mRNA processing and turnover. It functions mechanically within the RNA decay pathway aiding in the removal of the cap structure from mRNA a step important for mRNA degradation. EDC3 with a molecular mass of approximately 59 kDa is expressed in various tissues notably in brain and testis among others. It interacts with multiple RNA-binding proteins that support the assembly of mRNA decay machinery.

Biological function summary

EDC3 supports mRNA regulation by participating in mRNA decapping a regulatory process essential for controlling gene expression. EDC3 is a critical component of the decapping complex interacting with proteins like DCP1 and DCP2 facilitating efficient decapping of mRNA. The interaction of EDC3 with Pat1 another decapping activator enhances the mRNA decay process and ensures proper turnover of mRNA in the cell.

Pathways

EDC3 plays a part in the 5' to 3' mRNA decay pathway facilitating the removal of the cap structure leading to exonucleolytic degradation of mRNA. This pathway is important in mRNA degradation and turnover ensuring precise regulation of gene expression. EDC3 is related to proteins such as LSM1 and XRN1 which cooperate within the 5' to 3' exonucleolytic decay pathway promoting efficient mRNA degradation.

EDC3 alterations may associate with neurological conditions and cancer. Studies suggest that disruptions in mRNA decay where EDC3 plays a central role can lead to improper gene expression contributing to neuronal dysfunctions in neurological diseases. Also anomalies in decay pathways may link to oncogenic processes potentially pointing to EDC3's involvement in cancer development. EDC3’s interaction with proteins like DCP2 and XRN1 in the context of these disorders reveals the significance of RNA processing in maintaining cellular homeostasis and preventing disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds single-stranded RNA. Involved in the process of mRNA degradation and in the positive regulation of mRNA decapping. May play a role in spermiogenesis and oogenesis.

See full target information EDC3

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell 185:2035-2056.e33 PubMed35688132

2022

The Parkinson's disease protein alpha-synuclein is a modulator of processing bodies and mRNA stability.

Applications

Unspecified application

Species

Unspecified reactive species

Erinc Hallacli,Can Kayatekin,Sumaiya Nazeen,Xiou H Wang,Zoe Sheinkopf,Shubhangi Sathyakumar,Souvarish Sarkar,Xin Jiang,Xianjun Dong,Roberto Di Maio,Wen Wang,Matthew T Keeney,Daniel Felsky,Jackson Sandoe,Aazam Vahdatshoar,Namrata D Udeshi,D R Mani,Steven A Carr,Susan Lindquist,Philip L De Jager,David P Bartel,Chad L Myers,J Timothy Greenamyre,Mel B Feany,Shamil R Sunyaev,Chee Yeun Chung,Vikram Khurana

Cell chemical biology 28:463-474.e7 PubMed33357462

2020

Discovery of cellular substrates of human RNA-decapping enzyme DCP2 using a stapled bicyclic peptide inhibitor.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Luo,Jeremy A Schofield,Zhenkun Na,Tanja Hann,Matthew D Simon,Sarah A Slavoff

Molecular cell 68:144-157.e5 PubMed28965817

2017

P-Body Purification Reveals the Condensation of Repressed mRNA Regulons.

Applications

Unspecified application

Species

Unspecified reactive species

Arnaud Hubstenberger,Maïté Courel,Marianne Bénard,Sylvie Souquere,Michèle Ernoult-Lange,Racha Chouaib,Zhou Yi,Jean-Baptiste Morlot,Annie Munier,Magali Fradet,Maëlle Daunesse,Edouard Bertrand,Gérard Pierron,Julien Mozziconacci,Michel Kress,Dominique Weil

Nature chemical biology 13:174-180 PubMed27918561

2016

A human microprotein that interacts with the mRNA decapping complex.

Applications

Unspecified application

Species

Unspecified reactive species

Nadia G D'Lima,Jiao Ma,Lauren Winkler,Qian Chu,Ken H Loh,Elizabeth O Corpuz,Bogdan A Budnik,Jens Lykke-Andersen,Alan Saghatelian,Sarah A Slavoff

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EDC3 antibody

Immunoprecipitation - Anti-EDC3 antibody (AB168851)

Western blot - Anti-EDC3 antibody (AB168851)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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Primary Antibodies

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