Rabbit Polyclonal EDC3 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human EDC3 aa 1-100.
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Publications
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- Cell 185:2035-2056.e332022The Parkinson's disease protein alpha-synuclein is a modulator of processing bodies and mRNA stability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErinc Hallacli,Can Kayatekin,Sumaiya Nazeen,Xiou H Wang,Zoe Sheinkopf,Shubhangi Sathyakumar,Souvarish Sarkar,Xin Jiang,Xianjun Dong,Roberto Di Maio,Wen Wang,Matthew T Keeney,Daniel Felsky,Jackson Sandoe,Aazam Vahdatshoar,Namrata D Udeshi,D R Mani,Steven A Carr,Susan Lindquist,Philip L De Jager,David P Bartel,Chad L Myers,J Timothy Greenamyre,Mel B Feany,Shamil R Sunyaev,Chee Yeun Chung,Vikram KhuranaPubMed 35688132
- Cell chemical biology 28:463-474.e72020Discovery of cellular substrates of human RNA-decapping enzyme DCP2 using a stapled bicyclic peptide inhibitor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Luo,Jeremy A Schofield,Zhenkun Na,Tanja Hann,Matthew D Simon,Sarah A SlavoffPubMed 33357462
- Molecular cell 68:144-157.e52017P-Body Purification Reveals the Condensation of Repressed mRNA Regulons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArnaud Hubstenberger et. al.PubMed 28965817
- Nature chemical biology 13:174-1802016A human microprotein that interacts with the mRNA decapping complex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNadia G D'Lima,Jiao Ma,Lauren Winkler,Qian Chu,Ken H Loh,Elizabeth O Corpuz,Bogdan A Budnik,Jens Lykke-Andersen,Alan Saghatelian,Sarah A SlavoffPubMed 27918561
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human EDC3 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q96F86
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Chimpanzee | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Gorilla, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Gorilla, Orangutan | Dilution info - | Notes - |
Target data
Function
Binds single-stranded RNA. Involved in the process of mRNA degradation and in the positive regulation of mRNA decapping. May play a role in spermiogenesis and oogenesis.
Alternative names
LSM16, YJDC, YJEFN2, PP844, EDC3, Enhancer of mRNA-decapping protein 3, LSM16 homolog, YjeF N-terminal domain-containing protein 2, YjeF domain-containing protein 1, YjeF_N2, hYjeF_N2
Recommended products
Rabbit Polyclonal EDC3 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human EDC3 aa 1-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human EDC3 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q96F86
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab168851 was affinity purified using an epitope specific to EDC3 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The EDC3 protein also known as Enhancer of mRNA Decapping 3 serves an essential role in mRNA processing and turnover. It functions mechanically within the RNA decay pathway aiding in the removal of the cap structure from mRNA a step important for mRNA degradation. EDC3 with a molecular mass of approximately 59 kDa is expressed in various tissues notably in brain and testis among others. It interacts with multiple RNA-binding proteins that support the assembly of mRNA decay machinery.
- Biological function summary
EDC3 supports mRNA regulation by participating in mRNA decapping a regulatory process essential for controlling gene expression. EDC3 is a critical component of the decapping complex interacting with proteins like DCP1 and DCP2 facilitating efficient decapping of mRNA. The interaction of EDC3 with Pat1 another decapping activator enhances the mRNA decay process and ensures proper turnover of mRNA in the cell.
- Pathways
EDC3 plays a part in the 5' to 3' mRNA decay pathway facilitating the removal of the cap structure leading to exonucleolytic degradation of mRNA. This pathway is important in mRNA degradation and turnover ensuring precise regulation of gene expression. EDC3 is related to proteins such as LSM1 and XRN1 which cooperate within the 5' to 3' exonucleolytic decay pathway promoting efficient mRNA degradation.
- Associated diseases and disorders
EDC3 alterations may associate with neurological conditions and cancer. Studies suggest that disruptions in mRNA decay where EDC3 plays a central role can lead to improper gene expression contributing to neuronal dysfunctions in neurological diseases. Also anomalies in decay pathways may link to oncogenic processes potentially pointing to EDC3's involvement in cancer development. EDC3’s interaction with proteins like DCP2 and XRN1 in the context of these disorders reveals the significance of RNA processing in maintaining cellular homeostasis and preventing disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-EDC3 antibody (ab168851)
All lanes: Western blot - Anti-EDC3 antibody (ab168851) at 0.1 µg
Lane 1: 293T whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 50 µg
Lane 3: Jurkat whole cell lysate at 50 µg
Lane 4: TCMK-1 whole cell lysate at 50 µg
Lane 5: NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 56 kDa
Exposure time: 10s
Immunoprecipitation - Anti-EDC3 antibody (ab168851)
Detection of EDC3 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168851 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
Detection: Chemiluminescence with an exposure time of 10 secondsAll lanes: Immunoprecipitation - Anti-EDC3 antibody (ab168851)
Predicted band size: 56 kDa
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