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Rabbit Recombinant Monoclonal EDC4 antibody. Carrier free. Suitable for ICC/IF, IHC-P, WB and reacts with Human, Mouse samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDC4 antibody [EPR28929-24] - BSA and Azide free (AB321985), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDC4 antibody [EPR28929-24] - BSA and Azide free (AB321985), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-EDC4 antibody [EPR28929-24] - BSA and Azide free (AB321985), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-EDC4 antibody [EPR28929-24] - BSA and Azide free (AB321985), expandable thumbnail
  • Western blot - Anti-EDC4 antibody [EPR28929-24] - BSA and Azide free (AB321985), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIHC-PWB
Human
Tested
Tested
Tested
Mouse
Tested
Expected
Tested

Tested
Tested

Species
Human, Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human, Mouse
Dilution info
-
Notes

-

Target data

Function

In the process of mRNA degradation, seems to play a role in mRNA decapping. Component of a complex containing DCP2 and DCP1A which functions in decapping of ARE-containing mRNAs. Promotes complex formation between DCP1A and DCP2. Enhances the catalytic activity of DCP2 (in vitro).

Alternative names

HEDLS, EDC4, Enhancer of mRNA-decapping protein 4, Autoantigen Ge-1, Autoantigen RCD-8, Human enhancer of decapping large subunit, Hedls

Recommended products

Rabbit Recombinant Monoclonal EDC4 antibody. Carrier free. Suitable for ICC/IF, IHC-P, WB and reacts with Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR28929-24
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab321985 is the carrier-free version of Anti-EDC4 antibody [EPR28929-24] ab321984.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

EDC4 also known as Enhancer of decapping 4 plays an important role in RNA processing mechanisms. It weighs around 145 kDa. EDC4 is widely expressed in many tissues and cells providing integral support to cellular processes. This protein partners with other decapping proteins in cells to manage RNA turnover ensuring precise degradation of RNA molecules that are no longer needed. Its presence within human tissues suggests its broad involvement in regulating gene expression.

Biological function summary

EDC4 assists in the formation of the decapping complex. This complex facilitates the removal of the 5' cap from mRNAs an essential step in mRNA decay. This decapping activity influences mRNA stability and degradation allowing cells to modulate protein synthesis according to environmental changes. EDC4 interacts with other decapping components like DCP1 further stabilizing its function within the decapping complex and aiding cellular homeostasis.

Pathways

EDC4 plays a part in the mRNA surveillance pathway which ensures the fidelity of gene expression by eliminating faulty mRNA transcripts. Also it participates in the nonsense-mediated decay (NMD) pathway removing mRNA containing premature stop codons. It works alongside proteins such as UPF1 and UPF2 in these pathways to maintain mRNA quality and prevent production of truncated proteins that might disrupt cellular functions.

Associated diseases and disorders

Alterations in EDC4 expression can relate to neurodegenerative diseases such as Amyotrophic Lateral Sclerosis (ALS) and cancer. In particular the misregulation of mRNA decay processes can contribute to these conditions. EDC4 interacts with proteins like TDP-43 in the context of ALS where disrupted RNA metabolism features prominently in disease pathology. Understanding these interactions offers insight into therapeutically targeting the molecular mechanisms at play in these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDC4 antibody [EPR28929-24] - BSA and Azide free (ab321985), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDC4 antibody [EPR28929-24] - BSA and Azide free (ab321985)

    This data was developed using Anti-EDC4 antibody [EPR28929-24] ab321984, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling EDC4 with Anti-EDC4 antibody [EPR28929-24] ab321984 at 1/100 (5.04 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Punctate cytoplasmic staining on human colon carcinoma. The section was incubated with Anti-EDC4 antibody [EPR28929-24] ab321984 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDC4 antibody [EPR28929-24] - BSA and Azide free (ab321985), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDC4 antibody [EPR28929-24] - BSA and Azide free (ab321985)

    This data was developed using Anti-EDC4 antibody [EPR28929-24] ab321984, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling EDC4 with Anti-EDC4 antibody [EPR28929-24] ab321984 at 1/100 (5.04 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Punctate cytoplasmic staining on human tonsil. The section was incubated with Anti-EDC4 antibody [EPR28929-24] ab321984 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunocytochemistry/ Immunofluorescence - Anti-EDC4 antibody [EPR28929-24] - BSA and Azide free (ab321985), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-EDC4 antibody [EPR28929-24] - BSA and Azide free (ab321985)

    This data was developed using Anti-EDC4 antibody [EPR28929-24] ab321984, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized F9 (mouse embryonal carcinoma epithelial cell) cells labelling EDC4 with Anti-EDC4 antibody [EPR28929-24] ab321984 at 1/500 (1.008 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing puncta cytoplasmic staining in F9 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-EDC4 antibody [EPR28929-24] - BSA and Azide free (ab321985), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-EDC4 antibody [EPR28929-24] - BSA and Azide free (ab321985)

    This data was developed using Anti-EDC4 antibody [EPR28929-24] ab321984, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling EDC4 with Anti-EDC4 antibody [EPR28929-24] ab321984 at 1/500 (1.008 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing puncta cytoplasmic staining in HeLa cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Western blot - Anti-EDC4 antibody [EPR28929-24] - BSA and Azide free (ab321985), expandable thumbnail

    Western blot - Anti-EDC4 antibody [EPR28929-24] - BSA and Azide free (ab321985)

    This data was developed using Anti-EDC4 antibody [EPR28929-24] ab321984, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The bands beneath the target band (151 kDa) are likely to be degraded target fragments.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-EDC4 antibody [EPR28929-24] (Anti-EDC4 antibody [EPR28929-24] ab321984) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: HeLa transfected with siRNA specifically targeting EDC4 whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 151 kDa, 36 kDa

    Exposure time: 26s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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