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AB200645

Anti-EDEM1 antibody

4

(1 Review)

|

(2 Publications)

Rabbit Polyclonal EDEM1 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human EDEM1 aa 150-200.

View Alternative Names

EDEM, KIAA0212, EDEM1, ER degradation-enhancing alpha-mannosidase-like protein 1

1 Images
Western blot - Anti-EDEM1 antibody (AB200645)
  • WB

Supplier Data

Western blot - Anti-EDEM1 antibody (AB200645)

All lanes:

Western blot - Anti-EDEM1 antibody (ab200645)

Lane 1:

HEK293T whole cell lysate

Lane 2:

Raw264.7 whole cell lysate

Lane 3:

PC12 whole cell lysate

Predicted band size: 74 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human EDEM1 aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

Q92611

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab200645 was purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage buffer
pH: 7.2 Preservative: 0.097% Sodium azide Constituents: 99% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EDEM1 also known as ER degradation-enhancing alpha-mannosidase-like protein 1 is a protein that plays a role in the endoplasmic reticulum-associated degradation (ERAD) pathway. Its molecular mass is approximately 98 kDa. EDEM1 is expressed in various tissues but is highly present in the liver and pancreas. This protein helps to identify and target misfolded glycoproteins for degradation ensuring quality control within the endoplasmic reticulum.
Biological function summary

EDEM1 accelerates the removal of incorrectly folded proteins by promoting their retrotranslocation and subsequent degradation by the proteasome. It is not part of a stable complex but coordinates with ER chaperones to enhance the dislocation of substrates from the ER to the cytosol. EDEM1 regulates glycoprotein quality control helping to maintain proper protein homeostasis.

Pathways

EDEM1 contributes to the unfolded protein response (UPR) and the ERAD pathway. Both pathways are essential for managing protein folding and degradation within the cell. EDEM1 interacts with proteins like HERP and OS-9 during these processes helping with the recognition and processing of misfolded proteins. The correct functioning of these pathways is important for cellular adaptation to stress.

EDEM1's malfunction can link to conditions such as cystic fibrosis and neurodegenerative diseases. These diseases can arise due to defects in the clearance of misfolded proteins leading to their accumulation. EDEM1 interacts with proteins such as CFTR where improper folding is a known issue in cystic fibrosis. Its role in preventing the buildup of misfolded proteins makes it relevant to the pathology of these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Extracts misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle. It is directly involved in endoplasmic reticulum-associated degradation (ERAD) and targets misfolded glycoproteins for degradation in an N-glycan-independent manner, probably by forming a complex with SEL1L. It has low mannosidase activity, catalyzing mannose trimming from Man8GlcNAc2 to Man7GlcNAc2.
See full target information EDEM1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Aging 12: PubMed33461166

2021

Long intergenic non-coding RNA LINC00485 exerts tumor-suppressive activity by regulating miR-581/EDEM1 axis in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chenmeng Li,Bei Pan,Xiangxiang Liu,Jian Qin,Xuhong Wang,Bangshun He,Yuqin Pan,Huiling Sun,Tao Xu,Xueni Xu,Kaixuan Zeng,Shukui Wang

Scientific reports 8:5275 PubMed29588456

2018

Inhalational Anesthetics Induce Neuronal Protein Aggregation and Affect ER Trafficking.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew Coghlan,Elizabeth Richards,Sadiq Shaik,Pablo Rossi,Ramesh Babu Vanama,Saumel Ahmadi,Christelle Petroz,Mark Crawford,Jason T Maynes
View all publications
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