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AB174651

Anti-EDF1 antibody

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(8 Publications)

Rabbit Polyclonal EDF1 antibody. Suitable for IP, WB, IHC-Fr and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human EDF1 aa 50 to C-terminus.

View Alternative Names

Endothelial differentiation-related factor 1, EDF-1, Multiprotein-bridging factor 1, MBF1, EDF1

3 Images
Immunohistochemistry (Frozen sections) - Anti-EDF1 antibody (AB174651)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-EDF1 antibody (AB174651)

Immunohistochemical analysis of frozen section of Human breast carcinoma tissue with ab174651 at 1 : 1,000 (1μg/ml).

Detection : DAB

Western blot - Anti-EDF1 antibody (AB174651)
  • WB

Supplier Data

Western blot - Anti-EDF1 antibody (AB174651)

All lanes:

Western blot - Anti-EDF1 antibody (ab174651) at 0.1 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Predicted band size: 16 kDa

true

Exposure time: 3min

Immunoprecipitation - Anti-EDF1 antibody (AB174651)
  • IP

Supplier Data

Immunoprecipitation - Anti-EDF1 antibody (AB174651)

Detection of EDF1 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab174651 at 6 μg/mg lysate for IP (Lane 1). For WB detection ab174651 was used at 1 μg/ml. Lane 2 represents control IgG IP.

All lanes:

Immunoprecipitation - Anti-EDF1 antibody (ab174651)

Predicted band size: 16 kDa

true

Exposure time: 3s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-Fr, IP

applications

Immunogen

Synthetic Peptide within Human EDF1 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O60869

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500 - 1/2000", "IHCFr-species-notes": "<p>Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.</p>" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab174651 was affinity purified using an epitope specific to EDF1 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EDF1 also known as endothelial differentiation-related factor 1 is a well-characterized protein of approximately 15 kDa. It is broadly expressed in various tissues with a notable presence in endothelial cells. The protein participates in the regulation of several cellular processes by modulating gene expression. EDF1 acts as a transcriptional coactivator in processes such as cellular differentiation and proliferation.
Biological function summary

EDF1 plays an important role in modulating transcriptional activity related to endothelial cell differentiation. It does not seem to form part of a larger complex instead directly influencing nuclear receptors to drive expression changes. In particular EDF1 interacts with the retinoid X receptor (RXR) to regulate the transcription of target genes involved in cellular response and growth.

Pathways

EDF1 significantly impacts the retinoic acid signaling pathway and the nitric oxide signaling pathway. These pathways are important for processes such as cell proliferation and vascular development. EDF1's interaction with RXR suggests potential connections with other proteins such as peroxisome proliferator-activated receptors (PPARs) which play roles in lipid metabolism and inflammation control.

EDF1 relates to cardiovascular diseases and certain cancers. Changes in EDF1 expression or function can influence endothelial cell behavior contributing to the development of atherosclerosis or tumor angiogenesis. Additionally there is an interplay between EDF1 and other proteins like VEGF in the context of these diseases affecting angiogenic processes and progression of vascular abnormalities.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. May function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism.
See full target information EDF1
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:10350 PubMed39609413

2024

E3 ubiquitin ligase RNF10 promotes dissociation of stalled ribosomes and responds to ribosomal subunit imbalance.

Applications

Unspecified application

Species

Unspecified reactive species

Janina A Lehmann,Doris Lindner,Hsu-Min Sung,Georg Stoecklin

Nucleic acids research 52:12534-12548 PubMed39351862

2024

Ribosome Quality Control mitigates the cytotoxicity of ribosome collisions induced by 5-Fluorouracil.

Applications

Unspecified application

Species

Unspecified reactive species

Susanta Chatterjee,Parisa Naeli,Okan Onar,Nicole Simms,Aitor Garzia,Angela Hackett,Kelsey Coyle,Patric Harris Snell,Tom McGirr,Tanvi Nitin Sawant,Kexin Dang,Zornitsa Vasileva Stoichkova,Yumna Azam,Mark P Saunders,Michael Braun,Tommy Alain,Thomas Tuschl,Simon S McDade,Daniel B Longley,Christos G Gkogkas,Colin Adrain,John R P Knight,Seyed Mehdi Jafarnejad

RNA (New York, N.Y.) 30:1572-1585 PubMed39293933

2024

Translation elongation inhibitors stabilize select short-lived transcripts.

Applications

Unspecified application

Species

Unspecified reactive species

Nicolle A Rosa-Mercado,Allen R Buskirk,Rachel Green

Cell 187:3652-3670.e40 PubMed38843833

2024

The ribotoxic stress response drives UV-mediated cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Niladri K Sinha,Connor McKenney,Zhong Y Yeow,Jeffrey J Li,Ki Hong Nam,Tomer M Yaron-Barir,Jared L Johnson,Emily M Huntsman,Lewis C Cantley,Alban Ordureau,Sergi Regot,Rachel Green

Cell death & disease 14:467 PubMed37495584

2023

Nitric oxide-induced ribosome collision activates ribosomal surveillance mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Ryder,Frederic Schrøder Arendrup,José Francisco Martínez,Goda Snieckute,Chiara Pecorari,Riyaz Ahmad Shah,Anders H Lund,Melanie Blasius,Simon Bekker-Jensen

Cell metabolism 34:2036-2046.e8 PubMed36384144

2022

Ribosome stalling is a signal for metabolic regulation by the ribotoxic stress response.

Applications

Unspecified application

Species

Unspecified reactive species

Goda Snieckute,Aitana Victoria Genzor,Anna Constance Vind,Laura Ryder,Mark Stoneley,Sébastien Chamois,René Dreos,Cathrine Nordgaard,Frederike Sass,Melanie Blasius,Aida Rodríguez López,Sólveig Hlín Brynjólfsdóttir,Kasper Langebjerg Andersen,Anne E Willis,Lisa B Frankel,Steen Seier Poulsen,David Gatfield,Zachary Gerhart-Hines,Christoffer Clemmensen,Simon Bekker-Jensen

eLife 9: PubMed32744497

2020

EDF1 coordinates cellular responses to ribosome collisions.

Applications

Unspecified application

Species

Unspecified reactive species

Niladri K Sinha,Alban Ordureau,Katharina Best,James A Saba,Boris Zinshteyn,Elayanambi Sundaramoorthy,Amit Fulzele,Danielle M Garshott,Timo Denk,Matthias Thoms,Joao A Paulo,J Wade Harper,Eric J Bennett,Roland Beckmann,Rachel Green

Development (Cambridge, England) 145: PubMed29523653

2018

Mbf1 ensures Polycomb silencing by protecting mRNA from degradation by Pacman.

Applications

Unspecified application

Species

Unspecified reactive species

Kenichi Nishioka,Xian-Feng Wang,Hitomi Miyazaki,Hidenobu Soejima,Susumu Hirose
View all publications
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