Rabbit Recombinant Monoclonal EDG3/S1P3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 7 publications.
Images
![Western blot - Anti-EDG3/S1P3 antibody [EPR4541(2)] (AB126622), expandable thumbnail](https://content.abcam.com/products/images/edg3-s1p3-antibody-epr45412-ab126622--western-blot-img146326.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-EDG3/S1P3 antibody [EPR4541(2)] (AB126622)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG3/S1P3 antibody [EPR4541(2)] (AB126622), expandable thumbnail](https://content.abcam.com/products/images/edg3-s1p3-antibody-epr45412-ab126622--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img48717.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG3/S1P3 antibody [EPR4541(2)] (AB126622)")
![OI-RD Scanning - Anti-EDG3/S1P3 antibody [EPR4541(2)] (AB126622), expandable thumbnail](https://content.abcam.com/products/images/edg3-s1p3-antibody-epr45412-ab126622--oi-rd-scanning-img83430.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-EDG3/S1P3 antibody [EPR4541(2)] (AB126622)")
Publications
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- Disease markers 2022:91194232022Triptolide Inhibits the Biological Processes of HUVECs and HepG2 Cells via the Serine Palmitoyltransferase Long Chain Base Subunit 2/Sphingosine-1-Phosphate Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLulu Jia,Shengnan Zhu,Mingfei Zhu,Lingyue Huang,Siyuan Xu,Yuqin Luo,Juan Xiao,Huazhen Su,Shaoyuan Huang,Qinyou TanPubMed 36438896
- International journal of molecular sciences 23:2022Targeting SPHK1/PBX1 Axis Induced Cell Cycle Arrest in Non-Small Cell Lung Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhoujun Lin,Yin Li,Xiao Han,Zhenkun Fu,Zhenhuan Tian,Chenggang LiPubMed 36361531
- Experimental and therapeutic medicine 23:32021Hirudin, a thrombin inhibitor, attenuates TGF-β-induced fibrosis in renal proximal tubular epithelial cells by inhibition of protease-activated receptor 1 expression via S1P/S1PR2/S1PR3 signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiang Lin,Chunli Long,Zhengang Wang,Ronghui Wang,Wei Shi,Jiwei Qiu,Junlin Mo,Yongxiang XiePubMed 34815755
- Biochimica et biophysica acta. Molecular and cell 1865:1586322020Circulating cord blood HDL-S1P complex preserves the integrity of the feto-placental vasculature.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIlaria Del Gaudio et. al.PubMed 31954174
- Clinical & translational oncology : official publi 21:1240-12492019Differential expression of S1P receptor subtypes in human bladder transitional cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesA Palangi et. al.PubMed 30712233
- EBioMedicine 40:210-2232018S1P/S1PR3 axis promotes aerobic glycolysis by YAP/c-MYC/PGAM1 axis in osteosarcoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYifei Shen et. al.PubMed 30587459
- PloS one 12:e01823902017Unravelling the interplay of sphingolipids and TGF-β signaling in the human corneal stroma.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis.
Alternative names
C9orf108, C9orf47, EDG3, S1PR3, Sphingosine 1-phosphate receptor 3, S1P receptor 3, S1P3, Endothelial differentiation G-protein coupled receptor 3, Sphingosine 1-phosphate receptor Edg-3, S1P receptor Edg-3
Recommended products
Rabbit Recombinant Monoclonal EDG3/S1P3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 7 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR4541(2)
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 2.8 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EDG3 also known as S1P3 is a G protein-coupled receptor with a molecular mass of approximately 43 kDa. It interacts with sphingosine-1-phosphate (S1P) a bioactive lipid molecule involved in diverse cellular processes. EDG3/S1P3 is expressed in various tissues such as the cardiovascular system lungs and lymphoid organs highlighting its importance in different physiological contexts. As a member of the S1P receptor family it plays a significant role in cellular communication.
- Biological function summary
EDG3/S1P3 affects several critical processes such as cell proliferation survival and migration. It functions within complexes that modulate intracellular signaling pathways. The receptor is involved in the regulation of immune responses and vascular stability by mediating signals that maintain endothelial barrier function and lymphocyte trafficking. Its interactions with other S1P receptors also contribute to its broad influence on cellular functions.
- Pathways
EDG3/S1P3 participates in the sphingolipid signaling pathways and the PI3K-Akt pathway. These pathways are integral to cell growth and survival influencing various physiological responses. The receptor shares functional relationships with proteins such as EDG1/S1P1 and EDG5/S1P2 which also bind S1P and are involved in similar signaling cascades. These interactions align EDG3/S1P3 with essential cellular processes emphasizing its role in maintaining homeostasis.
- Associated diseases and disorders
EDG3/S1P3 has associations with cardiovascular diseases and cancer. Its role in endothelial barrier function ties it to cardiovascular health where dysregulation can lead to conditions such as atherosclerosis. In cancer abnormal EDG3/S1P3 signaling can contribute to tumor growth and metastasis by influencing cell migration and survival. The receptor's involvement in these diseases links it to other proteins like VEGF which are essential in angiogenesis and vascular maintenance highlighting its therapeutic potential.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab126622), expandable thumbnail](https://content.abcam.com/products/images/edg3-s1p3-antibody-epr45412-ab126622--western-blot-img146326.jpg "Western blot - Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab126622)")
Western blot - Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab126622)
All lanes: Western blot - Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab126622) at 1/1000 dilution
Lane 1: HepG2 cell lysate at 10 µg
Lane 2: Human heart tissue lysate at 10 µg
Lane 3: 293T cell lysate at 10 µg
SecondaryAll lanes: Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab126622), expandable thumbnail](https://content.abcam.com/products/images/edg3-s1p3-antibody-epr45412-ab126622--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img48717.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab126622)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab126622)
ab126622, at a 1/50 dilution, staining EDG3/S1P3 in paraffin-embedded Human kidney tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![OI-RD Scanning - Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab126622), expandable thumbnail](https://content.abcam.com/products/images/edg3-s1p3-antibody-epr45412-ab126622--oi-rd-scanning-img83430.jpg "OI-RD Scanning - Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab126622)")
OI-RD Scanning - Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab126622)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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