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AB248131

Anti-EDG3/S1P3 antibody [EPR4541(2)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal EDG3/S1P3 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

C9orf108, C9orf47, EDG3, S1PR3, Sphingosine 1-phosphate receptor 3, S1P receptor 3, S1P3, Endothelial differentiation G-protein coupled receptor 3, Sphingosine 1-phosphate receptor Edg-3, S1P receptor Edg-3

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG3/S1P3 antibody [EPR4541(2)] - BSA and Azide free (AB248131)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG3/S1P3 antibody [EPR4541(2)] - BSA and Azide free (AB248131)

This data was developed using ab126622, the same antibody clone in a different buffer formulation.

ab126622, at a 1/50 dilution, staining EDG3/S1P3 in paraffin-embedded Human kidney tissue by immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-EDG3/S1P3 antibody [EPR4541(2)] - BSA and Azide free (AB248131)
  • WB

Unknown

Western blot - Anti-EDG3/S1P3 antibody [EPR4541(2)] - BSA and Azide free (AB248131)

This data was developed using ab126622, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-EDG3/S1P3 antibody [EPR4541(2)] (<a href='/en-us/products/primary-antibodies/edg3-s1p3-antibody-epr45412-ab126622'>ab126622</a>) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

Human heart tissue lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Secondary

All lanes:

Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

OI-RD Scanning - Anti-EDG3/S1P3 antibody [EPR4541(2)] - BSA and Azide free (AB248131)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-EDG3/S1P3 antibody [EPR4541(2)] - BSA and Azide free (AB248131)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Unconjugated

    Anti-EDG3/S1P3 antibody [EPR4541(2)]

  • 660 APC

    APC Anti-EDG3/S1P3 antibody [EPR4541(2)]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-EDG3/S1P3 antibody [EPR4541(2)]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-EDG3/S1P3 antibody [EPR4541(2)]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-EDG3/S1P3 antibody [EPR4541(2)]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-EDG3/S1P3 antibody [EPR4541(2)]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-EDG3/S1P3 antibody [EPR4541(2)]

  • 578 PE

    PE Anti-EDG3/S1P3 antibody [EPR4541(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4541(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

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Secondary Antibodies

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Primary Antibodies

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  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab248131 is the carrier-free version of ab126622.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EDG3 also known as S1P3 is a G protein-coupled receptor with a molecular mass of approximately 43 kDa. It interacts with sphingosine-1-phosphate (S1P) a bioactive lipid molecule involved in diverse cellular processes. EDG3/S1P3 is expressed in various tissues such as the cardiovascular system lungs and lymphoid organs highlighting its importance in different physiological contexts. As a member of the S1P receptor family it plays a significant role in cellular communication.
Biological function summary

EDG3/S1P3 affects several critical processes such as cell proliferation survival and migration. It functions within complexes that modulate intracellular signaling pathways. The receptor is involved in the regulation of immune responses and vascular stability by mediating signals that maintain endothelial barrier function and lymphocyte trafficking. Its interactions with other S1P receptors also contribute to its broad influence on cellular functions.

Pathways

EDG3/S1P3 participates in the sphingolipid signaling pathways and the PI3K-Akt pathway. These pathways are integral to cell growth and survival influencing various physiological responses. The receptor shares functional relationships with proteins such as EDG1/S1P1 and EDG5/S1P2 which also bind S1P and are involved in similar signaling cascades. These interactions align EDG3/S1P3 with essential cellular processes emphasizing its role in maintaining homeostasis.

EDG3/S1P3 has associations with cardiovascular diseases and cancer. Its role in endothelial barrier function ties it to cardiovascular health where dysregulation can lead to conditions such as atherosclerosis. In cancer abnormal EDG3/S1P3 signaling can contribute to tumor growth and metastasis by influencing cell migration and survival. The receptor's involvement in these diseases links it to other proteins like VEGF which are essential in angiogenesis and vascular maintenance highlighting its therapeutic potential.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis.
See full target information S1PR3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:5945 PubMed36209205

2022

Hepatocyte phosphatase DUSP22 mitigates NASH-HCC progression by targeting FAK.

Applications

Unspecified application

Species

Unspecified reactive species

Chenxu Ge,Jun Tan,Xianling Dai,Qin Kuang,Shaoyu Zhong,Lili Lai,Chao Yi,Yan Sun,Jing Luo,Chufeng Zhang,Liancai Zhu,Bochu Wang,Minxuan Xu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com