AB140932

Anti-EDG8 antibody - N-terminal

Name: Anti-EDG8 antibody - N-terminal (ab140932) | Abcam
Brand: Abcam Limited
SKU: AB140932
Rating: 5 (3 reviews)

(3 Reviews)

(1 Publication)

Rabbit Polyclonal EDG8 antibody. Suitable for IHC-P and reacts with Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human S1PR5.

View Alternative Names

EDG8, S1PR5, Sphingosine 1-phosphate receptor 5, S1P receptor 5, S1P5, Endothelial differentiation G-protein-coupled receptor 8, Sphingosine 1-phosphate receptor Edg-8, S1P receptor Edg-8

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG8 antibody - N-terminal (AB140932)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG8 antibody - N-terminal (AB140932)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Rat brain (neurons and glia) tissue labelling EDG8 with ab140932 at 6 μg/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human S1PR5. The exact immunogen used to generate this antibody is proprietary information.

Q9H228

Specificity

BLAST analysis of the peptide immunogen showed no homology with other Human proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3-6 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." }, "Bat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Common marmoset": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Elephant": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EDG8 also known as S1P5 is a receptor with a mass of approximately 41 kDa. It belongs to the G-protein coupled receptor family and specifically binds to sphingosine-1-phosphate (S1P). Researchers often find EDG8 expression in tissues such as the central nervous system spleen and placenta. It plays an important role in mediating cellular responses to extracellular signals through its interaction with ligands.

Biological function summary

S1P5 functions in cellular growth survival and migration. It is part of a receptor complex that mediates immune response and neurologic functions. This receptor transmits signals that regulate cellular shape and cytoskeletal architecture. S1P5 influences various processes such as lymphocyte trafficking and oligodendrocyte maturation supporting its role in immune and neurological systems.

Pathways

EDG8 engages in the sphingolipid signaling pathway and is also important in the regulation of the immune system. This receptor interacts with other proteins like G-protein subunits which transduce signals from extracellular factors and promote intracellular responses. The protein S1P5 links to the Akt and MAPK pathways which are important for cell survival and proliferation. These pathways help mediate cellular responses through signal transduction and protein activation.

S1P5 associates notably with multiple sclerosis and some types of cancer. Dysfunction in this receptor's signaling can disrupt normal cell function and immune regulation. S1P5's interactions with proteins like S1P1 and S1P3 further implicate its role in the progression of these diseases. Research continues to explore how modulating EDG8 can offer therapeutic strategies for managing these conditions effectively.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. Is coupled to both the G(i/0)alpha and G(12) subclass of heteromeric G-proteins (By similarity). May play a regulatory role in the transformation of radial glial cells into astrocytes and may affect proliferative activity of these cells.

See full target information S1PR5

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The FEBS journal 281:2861-70 PubMed24798654

2014

Low-dose cytokine-induced neutral ceramidase secretion from INS-1 cells via exosomes and its anti-apoptotic effect.

Applications

Unspecified application

Species

Unspecified reactive species

Qun Zhu,Jian Kang,Heng Miao,Yamin Feng,Lijun Xiao,Zhigao Hu,Duan-Fang Liao,Yan Huang,Junfei Jin,Songqing He

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EDG8 antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG8 antibody - N-terminal (AB140932)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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