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AB190692

Anti-EDIL3/DEL1 antibody [EPR12451]

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(5 Publications)

Rabbit Recombinant Monoclonal EDIL3/DEL1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples. Cited in 5 publications.

View Alternative Names

DEL1, EDIL3, EGF-like repeat and discoidin I-like domain-containing protein 3, Developmentally-regulated endothelial cell locus 1 protein, Integrin-binding protein DEL1

5 Images
Flow Cytometry (Intracellular) - Anti-EDIL3/DEL1 antibody [EPR12451] (AB190692)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-EDIL3/DEL1 antibody [EPR12451] (AB190692)

Intracellular flow cytometric analysis of 2% paraformaldehyde HeLa cells labeling EDIL3/DEL1with ab190692 at 1/340 dilution (red) compared to a Rabbit monoclonal IgGcontrol antibody (green). Secondary staining with Goat anti Rabbit IgG (FITC).

Immunocytochemistry/ Immunofluorescence - Anti-EDIL3/DEL1 antibody [EPR12451] (AB190692)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EDIL3/DEL1 antibody [EPR12451] (AB190692)

Immunofluorescent staining of 4% paraformaldehyde fixed Hela cells labeling EDIL3/DEL1 with ab190692 at 1/250 dilution (green) and DAPI nuclear staining (blue). Secondary staining with Goat anti Rabbit IgG (Alexa Fluor® 488). The image also shows negative control cells.

Immunoprecipitation - Anti-EDIL3/DEL1 antibody [EPR12451] (AB190692)
  • IP

Supplier Data

Immunoprecipitation - Anti-EDIL3/DEL1 antibody [EPR12451] (AB190692)

Immunoprecipitation analysis of HeLa cell lysate labeling EDIL3/DEL1 with ab190692 at 1/50 dilution (lane 1) compared to a PBS control (lane 2). Secondary staining with anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG antibody at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-EDIL3/DEL1 antibody [EPR12451] (ab190692)

Predicted band size: 54 kDa

false

Western blot - Anti-EDIL3/DEL1 antibody [EPR12451] (AB190692)
  • WB

Supplier Data

Western blot - Anti-EDIL3/DEL1 antibody [EPR12451] (AB190692)

All lanes:

Western blot - Anti-EDIL3/DEL1 antibody [EPR12451] (ab190692) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

HUVEC cell lysate at 20 µg

Lane 3:

BxPC-3 cell lysate at 20 µg

Lane 4:

293 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 54 kDa

false

Western blot - Anti-EDIL3/DEL1 antibody [EPR12451] (AB190692)
  • WB

Supplier Data

Western blot - Anti-EDIL3/DEL1 antibody [EPR12451] (AB190692)

All lanes:

Western blot - Anti-EDIL3/DEL1 antibody [EPR12451] (ab190692) at 1/1000 dilution

Lane 1:

C6 cell lysate at 10 µg

Lane 2:

RAW 264.7 cell lysate at 10 µg

Lane 3:

PC-12 cell lysate at 10 µg

Lane 4:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 54 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12451

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IP, ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/340", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein EDIL3 also known as DEL1 or del-1 protein is a secreted extracellular matrix protein with a mass of approximately 52 kDa. It is expressed highly in endothelial cells and has a significant presence during embryonic development particularly within the arteries. As a versatile protein EDIL3 plays a role in promoting cell adhesion through interaction with integrins. This interaction makes it important for cellular communication and structural support in various tissues.
Biological function summary

EDIL3 influences vascular development and angiogenesis. It is not generally part of a large protein complex but works independently to support endothelial cell survival and proliferation. It binds preferentially to integrins like alphavbeta3 and alphavbeta5. This binding initiates intracellular signaling cascades that activate pathways responsible for cell growth and migration. EDIL3 therefore contributes to stabilizing newly formed blood vessels which is essential during developmental processes.

Pathways

EDIL3 plays a role in the regulation of several signaling networks including the MAPK and PI3K/AKT pathways. In these pathways it assists angiogenesis and endothelial cell function by activating signaling that promotes cell division and survival. EDIL3-related pathway interactions involve proteins such as VEGF an important factor in angiogenesis which also partners with integrins to enhance vascular development and repair processes.

EDIL3 has implications in cancer and inflammatory diseases. In cancer EDIL3 aids the formation of new blood vessels that supply nutrients to tumors promoting their growth and metastasis. It partners with the VEGF protein to enhance angiogenic processes in tumor environments. Inflammatory diseases such as rheumatoid arthritis show elevated levels of EDIL3 where it encourages pro-inflammatory responses through endothelial cell interactions. Here EDIL3 might interact with proteins involved in inflammatory pathways exacerbating disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Promotes adhesion of endothelial cells through interaction with the alpha-v/beta-3 integrin receptor. Inhibits formation of vascular-like structures. May be involved in regulation of vascular morphogenesis of remodeling in embryonic development.
See full target information EDIL3
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 15:1427661 PubMed39015570

2024

A novel prognostic signature related to programmed cell death in osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Chen Jiang,Qi-Tong Xu,Hong-Bin Wang,Si-Yuan Ren,Yao Zhang

iScience 27:108798 PubMed38261928

2024

A novel macrolide-Del-1 axis to regenerate bone in old age.

Applications

Unspecified application

Species

Unspecified reactive species

Kridtapat Sirisereephap,Hikaru Tamura,Jong-Hyung Lim,Meircurius Dwi Condro Surboyo,Toshihito Isono,Takumi Hiyoshi,Andrea L Rosenkranz,Yurie Sato-Yamada,Hisanori Domon,Akari Ikeda,Tomoyasu Hirose,Toshiaki Sunazuka,Nagako Yoshiba,Hiroyuki Okada,Yutaka Terao,Takeyasu Maeda,Koichi Tabeta,Triantafyllos Chavakis,George Hajishengallis,Tomoki Maekawa

Frontiers in ophthalmology 3: PubMed38223904

2024

Comparison of the extracellular vesicle proteome between glaucoma and non-glaucoma trabecular meshwork cells.

Applications

Unspecified application

Species

Unspecified reactive species

Fiona S McDonnell,Bre'Ida J Riddick,Haven Roberts,Nikolai Skiba,W Daniel Stamer

Nature communications 14:870 PubMed36797266

2023

Spatial proteomics reveals secretory pathway disturbances caused by neuropathy-associated TECPR2.

Applications

Unspecified application

Species

Unspecified reactive species

Karsten Nalbach,Martina Schifferer,Debjani Bhattacharya,Hung Ho-Xuan,Wei Chou Tseng,Luis A Williams,Alexandra Stolz,Stefan F Lichtenthaler,Zvulun Elazar,Christian Behrends

The Journal of cell biology 219: PubMed31757787

2019

Gene expression amplification by nuclear speckle association.

Applications

Unspecified application

Species

Unspecified reactive species

Jiah Kim,Neha Chivukula Venkata,Gabriela Andrea Hernandez Gonzalez,Nimish Khanna,Andrew S Belmont
View all publications
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Product promise

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