Skip to main content

Rabbit Recombinant Monoclonal EDIL3/DEL1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples. Cited in 1 publication.

Be the first to review this product! Submit a review

Images

Western blot - Anti-EDIL3/DEL1 antibody [EPR12451] (AB190692), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-EDIL3/DEL1 antibody [EPR12451] (AB190692), expandable thumbnail
  • Western blot - Anti-EDIL3/DEL1 antibody [EPR12451] (AB190692), expandable thumbnail
  • Immunoprecipitation - Anti-EDIL3/DEL1 antibody [EPR12451] (AB190692), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-EDIL3/DEL1 antibody [EPR12451] (AB190692), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Not recommended
Tested
Tested
Mouse
Expected
Tested
Not recommended
Expected
Expected
Rat
Expected
Tested
Not recommended
Expected
Expected

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/10000 - 1/50000
Notes

-

Species
Mouse
Dilution info
1/10000 - 1/50000
Notes

-

Species
Human
Dilution info
1/10000 - 1/50000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/250 - 1/500
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/340
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Promotes adhesion of endothelial cells through interaction with the alpha-v/beta-3 integrin receptor. Inhibits formation of vascular-like structures. May be involved in regulation of vascular morphogenesis of remodeling in embryonic development.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal EDIL3/DEL1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR12451
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein EDIL3 also known as DEL1 or del-1 protein is a secreted extracellular matrix protein with a mass of approximately 52 kDa. It is expressed highly in endothelial cells and has a significant presence during embryonic development particularly within the arteries. As a versatile protein EDIL3 plays a role in promoting cell adhesion through interaction with integrins. This interaction makes it important for cellular communication and structural support in various tissues.

Biological function summary

EDIL3 influences vascular development and angiogenesis. It is not generally part of a large protein complex but works independently to support endothelial cell survival and proliferation. It binds preferentially to integrins like alphavbeta3 and alphavbeta5. This binding initiates intracellular signaling cascades that activate pathways responsible for cell growth and migration. EDIL3 therefore contributes to stabilizing newly formed blood vessels which is essential during developmental processes.

Pathways

EDIL3 plays a role in the regulation of several signaling networks including the MAPK and PI3K/AKT pathways. In these pathways it assists angiogenesis and endothelial cell function by activating signaling that promotes cell division and survival. EDIL3-related pathway interactions involve proteins such as VEGF an important factor in angiogenesis which also partners with integrins to enhance vascular development and repair processes.

Associated diseases and disorders

EDIL3 has implications in cancer and inflammatory diseases. In cancer EDIL3 aids the formation of new blood vessels that supply nutrients to tumors promoting their growth and metastasis. It partners with the VEGF protein to enhance angiogenic processes in tumor environments. Inflammatory diseases such as rheumatoid arthritis show elevated levels of EDIL3 where it encourages pro-inflammatory responses through endothelial cell interactions. Here EDIL3 might interact with proteins involved in inflammatory pathways exacerbating disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-EDIL3/DEL1 antibody [EPR12451] (ab190692), expandable thumbnail

    Western blot - Anti-EDIL3/DEL1 antibody [EPR12451] (ab190692)

    All lanes: Western blot - Anti-EDIL3/DEL1 antibody [EPR12451] (ab190692) at 1/10000 dilution

    Lane 1: HeLa cell lysate at 20 µg

    Lane 2: HUVEC cell lysate at 20 µg

    Lane 3: BxPC-3 cell lysate at 20 µg

    Lane 4: 293 cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 54 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-EDIL3/DEL1 antibody [EPR12451] (ab190692), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-EDIL3/DEL1 antibody [EPR12451] (ab190692)

    Immunofluorescent staining of 4% paraformaldehyde fixed Hela cells labeling EDIL3/DEL1 with ab190692 at 1/250 dilution (green) and DAPI nuclear staining (blue). Secondary staining with Goat anti Rabbit IgG (Alexa Fluor® 488). The image also shows negative control cells.

  • Western blot - Anti-EDIL3/DEL1 antibody [EPR12451] (ab190692), expandable thumbnail

    Western blot - Anti-EDIL3/DEL1 antibody [EPR12451] (ab190692)

    All lanes: Western blot - Anti-EDIL3/DEL1 antibody [EPR12451] (ab190692) at 1/1000 dilution

    Lane 1: C6 cell lysate at 10 µg

    Lane 2: RAW 264.7 cell lysate at 10 µg

    Lane 3: PC-12 cell lysate at 10 µg

    Lane 4: NIH/3T3 cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 54 kDa

  • Immunoprecipitation - Anti-EDIL3/DEL1 antibody [EPR12451] (ab190692), expandable thumbnail

    Immunoprecipitation - Anti-EDIL3/DEL1 antibody [EPR12451] (ab190692)

    Immunoprecipitation analysis of HeLa cell lysate labeling EDIL3/DEL1 with ab190692 at 1/50 dilution (lane 1) compared to a PBS control (lane 2). Secondary staining with anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG antibody at 1/1500 dilution.

    All lanes: Immunoprecipitation - Anti-EDIL3/DEL1 antibody [EPR12451] (ab190692)

    Predicted band size: 54 kDa

  • Flow Cytometry (Intracellular) - Anti-EDIL3/DEL1 antibody [EPR12451] (ab190692), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-EDIL3/DEL1 antibody [EPR12451] (ab190692)

    Intracellular flow cytometric analysis of 2% paraformaldehyde HeLa cells labeling EDIL3/DEL1with ab190692 at 1/340 dilution (red) compared to a Rabbit monoclonal IgGcontrol antibody (green). Secondary staining with Goat anti Rabbit IgG (FITC).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com