Rabbit Recombinant Monoclonal EDIL3/DEL1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples. Cited in 1 publication.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
---|---|---|---|---|---|
Human | Tested | Tested | Not recommended | Tested | Tested |
Mouse | Expected | Tested | Not recommended | Expected | Expected |
Rat | Expected | Tested | Not recommended | Expected | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/30 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/10000 - 1/50000 | Notes - |
Species Mouse | Dilution info 1/10000 - 1/50000 | Notes - |
Species Human | Dilution info 1/10000 - 1/50000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/250 - 1/500 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/340 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
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Promotes adhesion of endothelial cells through interaction with the alpha-v/beta-3 integrin receptor. Inhibits formation of vascular-like structures. May be involved in regulation of vascular morphogenesis of remodeling in embryonic development.
DEL1, EDIL3, EGF-like repeat and discoidin I-like domain-containing protein 3, Developmentally-regulated endothelial cell locus 1 protein, Integrin-binding protein DEL1
Rabbit Recombinant Monoclonal EDIL3/DEL1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples. Cited in 1 publication.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
The protein EDIL3 also known as DEL1 or del-1 protein is a secreted extracellular matrix protein with a mass of approximately 52 kDa. It is expressed highly in endothelial cells and has a significant presence during embryonic development particularly within the arteries. As a versatile protein EDIL3 plays a role in promoting cell adhesion through interaction with integrins. This interaction makes it important for cellular communication and structural support in various tissues.
EDIL3 influences vascular development and angiogenesis. It is not generally part of a large protein complex but works independently to support endothelial cell survival and proliferation. It binds preferentially to integrins like alphavbeta3 and alphavbeta5. This binding initiates intracellular signaling cascades that activate pathways responsible for cell growth and migration. EDIL3 therefore contributes to stabilizing newly formed blood vessels which is essential during developmental processes.
EDIL3 plays a role in the regulation of several signaling networks including the MAPK and PI3K/AKT pathways. In these pathways it assists angiogenesis and endothelial cell function by activating signaling that promotes cell division and survival. EDIL3-related pathway interactions involve proteins such as VEGF an important factor in angiogenesis which also partners with integrins to enhance vascular development and repair processes.
EDIL3 has implications in cancer and inflammatory diseases. In cancer EDIL3 aids the formation of new blood vessels that supply nutrients to tumors promoting their growth and metastasis. It partners with the VEGF protein to enhance angiogenic processes in tumor environments. Inflammatory diseases such as rheumatoid arthritis show elevated levels of EDIL3 where it encourages pro-inflammatory responses through endothelial cell interactions. Here EDIL3 might interact with proteins involved in inflammatory pathways exacerbating disease progression.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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All lanes: Western blot - Anti-EDIL3/DEL1 antibody [EPR12451] (ab190692) at 1/10000 dilution
Lane 1: HeLa cell lysate at 20 µg
Lane 2: HUVEC cell lysate at 20 µg
Lane 3: BxPC-3 cell lysate at 20 µg
Lane 4: 293 cell lysate at 20 µg
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 54 kDa
Immunofluorescent staining of 4% paraformaldehyde fixed Hela cells labeling EDIL3/DEL1 with ab190692 at 1/250 dilution (green) and DAPI nuclear staining (blue). Secondary staining with Goat anti Rabbit IgG (Alexa Fluor® 488). The image also shows negative control cells.
All lanes: Western blot - Anti-EDIL3/DEL1 antibody [EPR12451] (ab190692) at 1/1000 dilution
Lane 1: C6 cell lysate at 10 µg
Lane 2: RAW 264.7 cell lysate at 10 µg
Lane 3: PC-12 cell lysate at 10 µg
Lane 4: NIH/3T3 cell lysate at 10 µg
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 54 kDa
Immunoprecipitation analysis of HeLa cell lysate labeling EDIL3/DEL1 with ab190692 at 1/50 dilution (lane 1) compared to a PBS control (lane 2). Secondary staining with anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG antibody at 1/1500 dilution.
All lanes: Immunoprecipitation - Anti-EDIL3/DEL1 antibody [EPR12451] (ab190692)
Predicted band size: 54 kDa
Intracellular flow cytometric analysis of 2% paraformaldehyde HeLa cells labeling EDIL3/DEL1with ab190692 at 1/340 dilution (red) compared to a Rabbit monoclonal IgGcontrol antibody (green). Secondary staining with Goat anti Rabbit IgG (FITC).
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