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AB251027

Anti-EDIL3/DEL1 antibody [EPR12451] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal EDIL3/DEL1 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples. Cited in 1 publication.

View Alternative Names

DEL1, EDIL3, EGF-like repeat and discoidin I-like domain-containing protein 3, Developmentally-regulated endothelial cell locus 1 protein, Integrin-binding protein DEL1

5 Images
Flow Cytometry (Intracellular) - Anti-EDIL3/DEL1 antibody [EPR12451] - BSA and Azide free (AB251027)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-EDIL3/DEL1 antibody [EPR12451] - BSA and Azide free (AB251027)

This data was developed using ab190692, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde HeLa cells labeling EDIL3/DEL1 with ab190692 at 1/340 dilution (red) compared to a Rabbit monoclonal IgGcontrol antibody (green). Secondary staining with Goat anti Rabbit IgG (FITC).

Immunocytochemistry/ Immunofluorescence - Anti-EDIL3/DEL1 antibody [EPR12451] - BSA and Azide free (AB251027)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EDIL3/DEL1 antibody [EPR12451] - BSA and Azide free (AB251027)

This data was developed using ab190692, the same antibody clone in a different buffer formulation.

Immunofluorescent staining of 4% paraformaldehyde fixed Hela cells labeling EDIL3/DEL1 with ab190692 at 1/250 dilution (green) and DAPI nuclear staining (blue). Secondary staining with Goat anti Rabbit IgG (Alexa Fluor® 488). The image also shows negative control cells.

Immunoprecipitation - Anti-EDIL3/DEL1 antibody [EPR12451] - BSA and Azide free (AB251027)
  • IP

Supplier Data

Immunoprecipitation - Anti-EDIL3/DEL1 antibody [EPR12451] - BSA and Azide free (AB251027)

This data was developed using ab190692, the same antibody clone in a different buffer formulation.

Immunoprecipitation analysis of HeLa cell lysate labeling EDIL3/DEL1 with ab190692 at 1/50 dilution (lane 1) compared to a PBS control (lane 2). Secondary staining with anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG antibody at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-EDIL3/DEL1 antibody [EPR12451] (<a href='/en-us/products/primary-antibodies/edil3-del1-antibody-epr12451-ab190692'>ab190692</a>)

Predicted band size: 54 kDa

false

Western blot - Anti-EDIL3/DEL1 antibody [EPR12451] - BSA and Azide free (AB251027)
  • WB

Supplier Data

Western blot - Anti-EDIL3/DEL1 antibody [EPR12451] - BSA and Azide free (AB251027)

This data was developed using ab190692, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-EDIL3/DEL1 antibody [EPR12451] (<a href='/en-us/products/primary-antibodies/edil3-del1-antibody-epr12451-ab190692'>ab190692</a>) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

HUVEC cell lysate at 20 µg

Lane 3:

BxPC-3 cell lysate at 20 µg

Lane 4:

293 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 54 kDa

false

Western blot - Anti-EDIL3/DEL1 antibody [EPR12451] - BSA and Azide free (AB251027)
  • WB

Supplier Data

Western blot - Anti-EDIL3/DEL1 antibody [EPR12451] - BSA and Azide free (AB251027)

This data was developed using ab190692, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-EDIL3/DEL1 antibody [EPR12451] (<a href='/en-us/products/primary-antibodies/edil3-del1-antibody-epr12451-ab190692'>ab190692</a>) at 1/1000 dilution

Lane 1:

C6 cell lysate at 10 µg

Lane 2:

RAW 264.7 cell lysate at 10 µg

Lane 3:

PC-12 cell lysate at 10 µg

Lane 4:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 54 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12451

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, WB, Flow Cyt (Intra), IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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Anti-Histone H3 (citrulline R2 + R8 + R17) antibody

Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (AB5103)

  • 4
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab251027 is the carrier-free version of ab190692.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein EDIL3 also known as DEL1 or del-1 protein is a secreted extracellular matrix protein with a mass of approximately 52 kDa. It is expressed highly in endothelial cells and has a significant presence during embryonic development particularly within the arteries. As a versatile protein EDIL3 plays a role in promoting cell adhesion through interaction with integrins. This interaction makes it important for cellular communication and structural support in various tissues.
Biological function summary

EDIL3 influences vascular development and angiogenesis. It is not generally part of a large protein complex but works independently to support endothelial cell survival and proliferation. It binds preferentially to integrins like alphavbeta3 and alphavbeta5. This binding initiates intracellular signaling cascades that activate pathways responsible for cell growth and migration. EDIL3 therefore contributes to stabilizing newly formed blood vessels which is essential during developmental processes.

Pathways

EDIL3 plays a role in the regulation of several signaling networks including the MAPK and PI3K/AKT pathways. In these pathways it assists angiogenesis and endothelial cell function by activating signaling that promotes cell division and survival. EDIL3-related pathway interactions involve proteins such as VEGF an important factor in angiogenesis which also partners with integrins to enhance vascular development and repair processes.

EDIL3 has implications in cancer and inflammatory diseases. In cancer EDIL3 aids the formation of new blood vessels that supply nutrients to tumors promoting their growth and metastasis. It partners with the VEGF protein to enhance angiogenic processes in tumor environments. Inflammatory diseases such as rheumatoid arthritis show elevated levels of EDIL3 where it encourages pro-inflammatory responses through endothelial cell interactions. Here EDIL3 might interact with proteins involved in inflammatory pathways exacerbating disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Promotes adhesion of endothelial cells through interaction with the alpha-v/beta-3 integrin receptor. Inhibits formation of vascular-like structures. May be involved in regulation of vascular morphogenesis of remodeling in embryonic development.
See full target information EDIL3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 21:454-462 PubMed31746378

2019

Blueberry anthocyanin‑enriched extract ameliorates transverse aortic constriction‑induced myocardial dysfunction via the DDAH1/ADMA/NO signaling pathway in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Weiqing Hu,Wenyue Wang,Qing Ma,Tao Liu,Jiefeng Zhang,Jicun Zhang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com