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AB224082

Anti-EDJ antibody

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(2 Publications)

Rabbit Polyclonal EDJ antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DNAJB11 aa 200 to C-terminus.

View Alternative Names

EDJ, ERJ3, HDJ9, PSEC0121, UNQ537/PRO1080, DNAJB11, DnaJ homolog subfamily B member 11, APOBEC1-binding protein 2, DnaJ protein homolog 9, ER-associated DNAJ, ER-associated Hsp40 co-chaperone, Endoplasmic reticulum DNA J domain-containing protein 3, HEDJ, Human DnaJ protein 9, PWP1-interacting protein 4, ABBP-2, ER-resident protein ERdj3, ERdj3, ERj3p, hDj-9

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDJ antibody (AB224082)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDJ antibody (AB224082)

Paraffin embedded human pancreas tissue stained for EDJ with ab224082 (1/200 dilution) in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-EDJ antibody (AB224082)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-EDJ antibody (AB224082)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for EDJ (green) using ab224082 (4 μg/ml) in ICC/IF.

Western blot - Anti-EDJ antibody (AB224082)
  • WB

Supplier Data

Western blot - Anti-EDJ antibody (AB224082)

All lanes:

Western blot - Anti-EDJ antibody (ab224082) at 1/100 dilution

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Predicted band size: 40 kDa

false

Western blot - Anti-EDJ antibody (AB224082)
  • WB

Supplier Data

Western blot - Anti-EDJ antibody (AB224082)

All lanes:

Western blot - Anti-EDJ antibody (ab224082) at 1/100 dilution

Lane 1:

NIH/3T3 (mouse embyro fibroblast cell line) cell lysate

Lane 2:

NBT-II (rat cell line) cell lysate

Predicted band size: 40 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human DNAJB11 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UBS4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EDJ also known as Endoplasmic Reticulum DNAJ homolog or ERdj is a chaperone protein with a molecular mass of approximately 40 kDa. It is predominantly expressed in the endoplasmic reticulum (ER) of cells. EDJ plays a role in assisting unfolded or misfolded proteins to attain their correct structure. It belongs to the DNAJ family of co-chaperones and collaborates with other proteins particularly Hsp70 to ensure protein quality control within the ER.
Biological function summary

EDJ facilitates protein folding by stabilizing unfolded protein states and preventing protein aggregation. It acts as part of a larger multiprotein complex which is essential for maintaining cellular homeostasis. The chaperone activity of EDJ enables cells to cope with stress conditions such as those caused by ER stress or the accumulation of unfolded proteins. This involvement highlights its participation in the unfolded protein response (UPR) mechanism which restores normal cellular function in stressed cells.

Pathways

EDJ plays a significant role in the unfolded protein response and the heat shock response pathways. In these pathways EDJ interacts with proteins like BiP/GRP78 contributing to the regulatory mechanisms that maintain protein equilibrium within the ER. The protein helps manage these pathways by modulating the activity of stress sensors that initiate adaptive responses when proteins lose their proper folding within the ER.

EDJ has been implicated in the progression of neurodegenerative diseases and cancer. Its interaction with proteins such as ATF6 and IRE1 links it to processes that potentially lead to cell survival or apoptosis under pathological conditions. In neurodegenerative diseases mismanagement of protein folding and quality control where EDJ holds importance can result in protein aggregation. In cancer the regulation of ER stress pathways by EDJ may contribute to cancer cell survival under adverse conditions providing potential targets for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

As a co-chaperone for HSPA5 it is required for proper folding, trafficking or degradation of proteins (PubMed : 10827079, PubMed : 15525676, PubMed : 29706351). Binds directly to both unfolded proteins that are substrates for ERAD and nascent unfolded peptide chains, but dissociates from the HSPA5-unfolded protein complex before folding is completed (PubMed : 15525676). May help recruiting HSPA5 and other chaperones to the substrate. Stimulates HSPA5 ATPase activity (PubMed : 10827079). It is necessary for maturation and correct trafficking of PKD1 (PubMed : 29706351).
See full target information DNAJB11
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica communications 12:140 PubMed39198924

2024

A deep phenotyping study in mouse and iPSC models to understand the role of oligodendroglia in optic neuropathy in Wolfram syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

K Ahuja,M Vandenabeele,F Nami,E Lefevere,J Van Hoecke,S Bergmans,M Claes,T Vervliet,K Neyrinck,T Burg,D De Herdt,P Bhaskar,Y Zhu,Z J Looser,J Loncke,W Gsell,M Plaas,P Agostinis,J V Swinnen,L Van Den Bosch,G Bultynck,A S Saab,E Wolfs,Y C Chai,U Himmelreich,C Verfaillie,L Moons,L De Groef

International journal of molecular sciences 23: PubMed35008858

2021

New Proteins Contributing to Immune Cell Infiltration and Pannus Formation of Synovial Membrane from Arthritis Diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Dominique de Seny,Dominique Baiwir,Elettra Bianchi,Gaël Cobraiville,Céline Deroyer,Christophe Poulet,Olivier Malaise,Geneviève Paulissen,Marie-Joëlle Kaiser,Jean-Philippe Hauzeur,Gabriel Mazzucchelli,Philippe Delvenne,Michel Malaise
View all publications
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