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AB75107

Anti-EDJ antibody

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(3 Publications)

Rabbit Polyclonal EDJ antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human DNAJB11 aa 1-100.

View Alternative Names

EDJ, ERJ3, HDJ9, PSEC0121, UNQ537/PRO1080, DNAJB11, DnaJ homolog subfamily B member 11, APOBEC1-binding protein 2, DnaJ protein homolog 9, ER-associated DNAJ, ER-associated Hsp40 co-chaperone, Endoplasmic reticulum DNA J domain-containing protein 3, HEDJ, Human DnaJ protein 9, PWP1-interacting protein 4, ABBP-2, ER-resident protein ERdj3, ERdj3, ERj3p, hDj-9

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDJ antibody (AB75107)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDJ antibody (AB75107)

Immunohistochemical analysis of EDJ in paraffin embedded human heart tissue using 1/50 ab75107.
Left image : untreated sample.
Right image : sample treated with immunising peptide.

Western blot - Anti-EDJ antibody (AB75107)
  • WB

Unknown

Western blot - Anti-EDJ antibody (AB75107)

All lanes:

Western blot - Anti-EDJ antibody (ab75107) at 1/500 dilution

Lane 1:

extracts from HeLa cells at 30 µg

Lane 2:

extracts from HUVECcells at 30 µg

Lane 3:

extracts from COLO cells at 30 µg

Lane 4:

extracts from MCF-7 cells at 30 µg

Lane 5:

extracts from HeLa cells at 30 µg with immunising peptide

Predicted band size: 40 kDa

Observed band size: 40 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human DNAJB11 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q9UBS4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EDJ also known as Endoplasmic Reticulum DNAJ homolog or ERdj is a chaperone protein with a molecular mass of approximately 40 kDa. It is predominantly expressed in the endoplasmic reticulum (ER) of cells. EDJ plays a role in assisting unfolded or misfolded proteins to attain their correct structure. It belongs to the DNAJ family of co-chaperones and collaborates with other proteins particularly Hsp70 to ensure protein quality control within the ER.
Biological function summary

EDJ facilitates protein folding by stabilizing unfolded protein states and preventing protein aggregation. It acts as part of a larger multiprotein complex which is essential for maintaining cellular homeostasis. The chaperone activity of EDJ enables cells to cope with stress conditions such as those caused by ER stress or the accumulation of unfolded proteins. This involvement highlights its participation in the unfolded protein response (UPR) mechanism which restores normal cellular function in stressed cells.

Pathways

EDJ plays a significant role in the unfolded protein response and the heat shock response pathways. In these pathways EDJ interacts with proteins like BiP/GRP78 contributing to the regulatory mechanisms that maintain protein equilibrium within the ER. The protein helps manage these pathways by modulating the activity of stress sensors that initiate adaptive responses when proteins lose their proper folding within the ER.

EDJ has been implicated in the progression of neurodegenerative diseases and cancer. Its interaction with proteins such as ATF6 and IRE1 links it to processes that potentially lead to cell survival or apoptosis under pathological conditions. In neurodegenerative diseases mismanagement of protein folding and quality control where EDJ holds importance can result in protein aggregation. In cancer the regulation of ER stress pathways by EDJ may contribute to cancer cell survival under adverse conditions providing potential targets for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

As a co-chaperone for HSPA5 it is required for proper folding, trafficking or degradation of proteins (PubMed : 10827079, PubMed : 15525676, PubMed : 29706351). Binds directly to both unfolded proteins that are substrates for ERAD and nascent unfolded peptide chains, but dissociates from the HSPA5-unfolded protein complex before folding is completed (PubMed : 15525676). May help recruiting HSPA5 and other chaperones to the substrate. Stimulates HSPA5 ATPase activity (PubMed : 10827079). It is necessary for maturation and correct trafficking of PKD1 (PubMed : 29706351).
See full target information DNAJB11
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

American journal of human genetics 102:832-844 PubMed29706351

2018

Monoallelic Mutations to DNAJB11 Cause Atypical Autosomal-Dominant Polycystic Kidney Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Emilie Cornec-Le Gall,Rory J Olson,Whitney Besse,Christina M Heyer,Vladimir G Gainullin,Jessica M Smith,Marie-Pierre Audrézet,Katharina Hopp,Binu Porath,Beili Shi,Saurabh Baheti,Sarah R Senum,Jennifer Arroyo,Charles D Madsen,Claude Férec,Dominique Joly,François Jouret,Oussamah Fikri-Benbrahim,Christophe Charasse,Jean-Marie Coulibaly,Alan S Yu,Korosh Khalili,York Pei,Stefan Somlo,Yannick Le Meur,Vicente E Torres,Peter C Harris

International journal of cancer 135:2878-86 PubMed24771638

2014

Quantitative proteome profiling of lymph node-positive vs. -negative colorectal carcinomas pinpoints MX1 as a marker for lymph node metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Roland S Croner,Michael Stürzl,Tilman T Rau,Gergana Metodieva,Carol I Geppert,Elisabeth Naschberger,Berthold Lausen,Metodi V Metodiev

Biology of reproduction 89:142 PubMed24198121

2013

Cigarette smoking exposure alters pebp1 DNA methylation and protein profile involved in MAPK signaling pathway in mice testis.

Applications

Unspecified application

Species

Unspecified reactive species

Wangjie Xu,Peng Fang,Zijue Zhu,Jingbo Dai,Dongsheng Nie,Zhong Chen,Qiaojing Qin,Lianyun Wang,Zhaoxia Wang,Zhongdong Qiao
View all publications
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