Rabbit Polyclonal EDJ antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human DNAJB11 aa 1-100.
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Publications
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- American journal of human genetics 102:832-8442018Monoallelic Mutations to DNAJB11 Cause Atypical Autosomal-Dominant Polycystic Kidney Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmilie Cornec-Le Gall,Rory J Olson,Whitney Besse,Christina M Heyer,Vladimir G Gainullin,Jessica M Smith,Marie-Pierre Audrézet,Katharina Hopp,Binu Porath,Beili Shi,Saurabh Baheti,Sarah R Senum,Jennifer Arroyo,Charles D Madsen,Claude Férec,Dominique Joly,François Jouret,Oussamah Fikri-Benbrahim,Christophe Charasse,Jean-Marie Coulibaly,Alan S Yu,Korosh Khalili,York Pei,Stefan Somlo,Yannick Le Meur,Vicente E Torres,Peter C HarrisPubMed 29706351
- International journal of cancer 135:2878-862014Quantitative proteome profiling of lymph node-positive vs. -negative colorectal carcinomas pinpoints MX1 as a marker for lymph node metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRoland S Croner et. al.PubMed 24771638
- Biology of reproduction 89:1422013Cigarette smoking exposure alters pebp1 DNA methylation and protein profile involved in MAPK signaling pathway in mice testis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWangjie Xu et. al.PubMed 24198121
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human DNAJB11 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UBS4
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
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Target data
Function
As a co-chaperone for HSPA5 it is required for proper folding, trafficking or degradation of proteins (PubMed:10827079, PubMed:15525676, PubMed:29706351). Binds directly to both unfolded proteins that are substrates for ERAD and nascent unfolded peptide chains, but dissociates from the HSPA5-unfolded protein complex before folding is completed (PubMed:15525676). May help recruiting HSPA5 and other chaperones to the substrate. Stimulates HSPA5 ATPase activity (PubMed:10827079). It is necessary for maturation and correct trafficking of PKD1 (PubMed:29706351).
Alternative names
EDJ, ERJ3, HDJ9, PSEC0121, UNQ537/PRO1080, DNAJB11, DnaJ homolog subfamily B member 11, APOBEC1-binding protein 2, DnaJ protein homolog 9, ER-associated DNAJ, ER-associated Hsp40 co-chaperone, Endoplasmic reticulum DNA J domain-containing protein 3, HEDJ, Human DnaJ protein 9, PWP1-interacting protein 4, ABBP-2, ER-resident protein ERdj3, ERdj3, ERj3p, hDj-9
Recommended products
Rabbit Polyclonal EDJ antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human DNAJB11 aa 1-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human DNAJB11 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UBS4
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EDJ also known as Endoplasmic Reticulum DNAJ homolog or ERdj is a chaperone protein with a molecular mass of approximately 40 kDa. It is predominantly expressed in the endoplasmic reticulum (ER) of cells. EDJ plays a role in assisting unfolded or misfolded proteins to attain their correct structure. It belongs to the DNAJ family of co-chaperones and collaborates with other proteins particularly Hsp70 to ensure protein quality control within the ER.
- Biological function summary
EDJ facilitates protein folding by stabilizing unfolded protein states and preventing protein aggregation. It acts as part of a larger multiprotein complex which is essential for maintaining cellular homeostasis. The chaperone activity of EDJ enables cells to cope with stress conditions such as those caused by ER stress or the accumulation of unfolded proteins. This involvement highlights its participation in the unfolded protein response (UPR) mechanism which restores normal cellular function in stressed cells.
- Pathways
EDJ plays a significant role in the unfolded protein response and the heat shock response pathways. In these pathways EDJ interacts with proteins like BiP/GRP78 contributing to the regulatory mechanisms that maintain protein equilibrium within the ER. The protein helps manage these pathways by modulating the activity of stress sensors that initiate adaptive responses when proteins lose their proper folding within the ER.
- Associated diseases and disorders
EDJ has been implicated in the progression of neurodegenerative diseases and cancer. Its interaction with proteins such as ATF6 and IRE1 links it to processes that potentially lead to cell survival or apoptosis under pathological conditions. In neurodegenerative diseases mismanagement of protein folding and quality control where EDJ holds importance can result in protein aggregation. In cancer the regulation of ER stress pathways by EDJ may contribute to cancer cell survival under adverse conditions providing potential targets for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-EDJ antibody (ab75107)
All lanes: Western blot - Anti-EDJ antibody (ab75107) at 1/500 dilution
Lane 1: extracts from HeLa cells at 30 µg
Lane 2: extracts from HUVECcells at 30 µg
Lane 3: extracts from COLO cells at 30 µg
Lane 4: extracts from MCF-7 cells at 30 µg
Lane 5: extracts from HeLa cells at 30 µg with immunising peptide
Predicted band size: 40 kDa
Observed band size: 40 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDJ antibody (ab75107)
Immunohistochemical analysis of EDJ in paraffin embedded human heart tissue using 1/50 ab75107.
Left image: untreated sample.
Right image: sample treated with immunising peptide.
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