AB175424

Anti-EDR1 / PHC1 antibody [1F3F3]

Name: Anti-EDR1 / PHC1 antibody [1F3F3] (ab175424) | Abcam
Brand: Abcam Limited
SKU: AB175424
Price: 520 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal EDR1 / PHC1 antibody. Suitable for Flow Cyt, WB and reacts with Human, Recombinant fragment - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PHC1 aa 750 to C-terminus.

View Alternative Names

EDR1, PH1, PHC1, Polyhomeotic-like protein 1, hPH1, Early development regulatory protein 1

3 Images

Flow Cytometry - Anti-EDR1 / PHC1 antibody [1F3F3] (AB175424)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-EDR1 / PHC1 antibody [1F3F3] (AB175424)

Flow cytometric analysis of HEK293 cells labeling EDR1 / PHC1 with ab175424 at 1/200 dilution (green) and negative control (red).

Supplier Data

Western blot - Anti-EDR1 / PHC1 antibody [1F3F3] (AB175424)

All lanes:

Western blot - Anti-EDR1 / PHC1 antibody [1F3F3] (ab175424) at 1/500 dilution

All lanes:

EDR1/PHC1 recombinant protein (amino acids 758-1004)

Predicted band size: 106 kDa

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Supplier Data

Western blot - Anti-EDR1 / PHC1 antibody [1F3F3] (AB175424)

All lanes:

Western blot - Anti-EDR1 / PHC1 antibody [1F3F3] (ab175424) at 1/500 dilution

Lane 1:

HEK293 cell lysate

Lane 2:

EDR1/PHC1 (amino acids 758-1004)-hIgGFc transfected HEK293 cell lysate

Predicted band size: 106 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1F3F3

Isotype

IgG2b

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human PHC1 aa 750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P78364

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: 99% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EDR1 also known as PHC1 is a protein that functions mechanically by participating in chromatin modification. Its molecular mass is approximately 105 kDa. The protein is expressed in various tissues with significant presence noted in proliferative cells. PHC1 is commonly referred to as a member of the Polycomb group proteins which are essential in regulating gene expression by modifying chromatin structure influencing the accessibility of DNA for transcription. It plays a critical role in the maintenance of repression states of homeotic genes during development.

Biological function summary

EDR1/PHC1 is involved in maintaining cellular identity and proliferation by forming part of the Polycomb repressive complex 1 (PRC1). This complex facilitates the ubiquitination of histone H2A leading to transcriptional repression. PHC1 influences the silencing of specific target genes that are important in developmental pathways. It also plays a role in the regulation of stem cell pluripotency and differentiation contributing to the epigenetic memory of cells that persist through cell divisions.

Pathways

PHC1 significantly influences gene silencing through its involvement in the Polycomb repressive complex 1 pathway interacting with other Polycomb-related proteins like RING1 and BMI1. The protein is key in the regulation of the Wnt signaling pathway impacting the balance between cell differentiation and proliferation. PHC1 affects this pathway by modulating the expression of genes that are important for cell fate determination. Its interaction with BMI1 and other Polycomb group proteins ensures the proper progression of these pathways.

Defects in PHC1 contribute to leukaemias and neural developmental disorders. Mutations within the PHC1 gene have been associated with disrupted gene silencing and aberrant expression profiles contributing to the etiology of these conditions. In leukaemias its interaction with BMI1 is important as the dysregulation of this complex can lead to uncontrolled cell proliferation. In neural developmental disorders PHC1 affects the expression of key regulatory genes further highlighting its importance in normal cellular function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Required for proper control of cellular levels of GMNN expression.

See full target information PHC1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Arthritis & rheumatology (Hoboken, N.J.) 67:1250-60 PubMed25604648

2015

Contribution of the Interleukin-6/STAT-3 Signaling Pathway to Chondrogenic Differentiation of Human Mesenchymal Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Masahiro Kondo,Kunihiro Yamaoka,Kei Sakata,Koshiro Sonomoto,Lin Lin,Kazuhisa Nakano,Yoshiya Tanaka

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EDR1 / PHC1 antibody [1F3F3]

Flow Cytometry - Anti-EDR1 / PHC1 antibody [1F3F3] (AB175424)

Western blot - Anti-EDR1 / PHC1 antibody [1F3F3] (AB175424)

Western blot - Anti-EDR1 / PHC1 antibody [1F3F3] (AB175424)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

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