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AB70521

Anti-EEA1 antibody [1G11] - Early Endosome Marker

3

(8 Reviews)

|

(92 Publications)

Mouse Monoclonal EEA1 antibody. Early Endosome marker. Suitable for ICC/IF and reacts with Human samples. Cited in 92 publications.

View Alternative Names

ZFYVE2, EEA1, Early endosome antigen 1, Endosome-associated protein p162, Zinc finger FYVE domain-containing protein 2

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [1G11] - Early Endosome Marker (AB70521)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [1G11] - Early Endosome Marker (AB70521)

ab70521 staining EEA1 in HepG2 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab70521 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Also suitable in cells fixed with 100% methanol (5 min).Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1G11

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-5 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EEA1 (Early Endosome Antigen 1) is an important protein involved in endosomal trafficking with a molecular weight of approximately 162 kDa. It is a cytoplasmic protein expressed in various cell types acting mainly as an endosome marker. This protein contains a FYVE domain which facilitates binding to phosphatidylinositol 3-phosphate on endosomal membranes. EEA1 also interacts with Rab5 a small GTPase contributing to the fusion of early endosomes.
Biological function summary

EEA1 plays a significant role in early endosomal pathways facilitating endocytic vesicle fusion. EEA1 does not usually work alone; it functions as part of a larger complex involving other endosomal proteins. This protein acts as a tethering molecule which stabilizes the interaction of vesicles with endosomes. By anchoring vesicles EEA1 ensures the correct delivery and sorting of cargo within the cell which is important for cellular homeostasis and signaling.

Pathways

EEA1 contributes to the endocytic pathway and receptor recycling. It ensures proper functioning of the endocytic recycling of receptors such as the epidermal growth factor receptor (EGFR). EEA1-mediated tethering is vital for these pathways and it works closely with proteins like Rab5 which regulate endocytic trafficking. Through these interactions EEA1 affects cellular processes by controlling the flow of signaling receptors and other molecules within the cell.

EEA1 dysfunction is linked to neurodegenerative diseases and cancer. Abnormalities in its expression or function can disrupt endosomal trafficking leading to cellular stress and disease. For example in Alzheimer's disease impaired endosomal function involving EEA1 can contribute to amyloid-beta accumulation. In cancer altered EEA1 activity may contribute to aberrant receptor signaling which can promote unchecked cell division. The interplay with proteins like EGFR demonstrates EEA1's importance in maintaining cellular health and response mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
See full target information EEA1
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Publications (92)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:655 PubMed40877231

2025

Targeting the SYVN1-EGFR axis: a breakthrough strategy for TKI-resistant NSCLC.

Applications

Unspecified application

Species

Unspecified reactive species

Xinsheng Xie,Weilai Tong,Yue Xie,Haoxin Jiang,Alan Jiang,Junming Huang,Zhili Liu,Jingjing Yu

The Journal of biological chemistry 301:110383 PubMed40523617

2025

FAK activity exacerbates disturbed flow-mediated atherosclerosis via VEGFR2-CBL-NF-κB signaling.

Applications

Unspecified application

Species

Unspecified reactive species

James M Murphy,Duyen Thi Kieu Tran,Kyuho Jeong,Ly Nguyen,Mai Thi Nguyen,Dhananjay Tambe,Hanjoong Jo,Eun-Young Erin Ahn,Ssang-Taek Steve Lim

Nature 642:492-500 PubMed40335696

2025

Activation of lysosomal iron triggers ferroptosis in cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tatiana Cañeque,Leeroy Baron,Sebastian Müller,Alanis Carmona,Ludovic Colombeau,Antoine Versini,Stéphanie Solier,Christine Gaillet,Fabien Sindikubwabo,Julio L Sampaio,Marie Sabatier,Eikan Mishima,Armel Picard-Bernes,Laurène Syx,Nicolas Servant,Bérangère Lombard,Damarys Loew,Jiashuo Zheng,Bettina Proneth,Leishemba K Thoidingjam,Laurence Grimaud,Cameron S Fraser,Krystina J Szylo,Emma Der Kazarian,Caroline Bonnet,Emmanuelle Charafe-Jauffret,Christophe Ginestier,Patricia Santofimia-Castaño,Matias Estaras,Nelson Dusetti,Juan Lucio Iovanna,Antonio Sa Cunha,Gabriella Pittau,Pascal Hammel,Dimitri Tzanis,Sylvie Bonvalot,Sarah Watson,Vincent Gandon,Aditya Upadhyay,Derek A Pratt,Florêncio Porto Freitas,José Pedro Friedmann Angeli,Brent R Stockwell,Marcus Conrad,Jessalyn M Ubellacker,Raphaël Rodriguez

Advanced materials (Deerfield Beach, Fla.) 36:e2408000 PubMed39417762

2024

Unlocking Intracellular Protein Delivery by Harnessing Polymersomes Synthesized at Microliter Volumes using Photo-PISA.

Applications

Unspecified application

Species

Unspecified reactive species

Chalaisorn Thanapongpibul,Omar Rifaie-Graham,Miina Ojansivu,Adrian Najer,Hyemin Kim,Saskia E Bakker,Mohamed Chami,David J Peeler,Chenchen Liu,Jonathan Yeow,Molly M Stevens

Nature communications 15:5678 PubMed38971858

2024

Erythroid-intrinsic activation of TLR8 impairs erythropoiesis in inherited anemia.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Liang,Yang Wan,Jie Gao,Lingyue Zheng,Jingwei Wang,Peng Wu,Yue Li,Bingrui Wang,Ding Wang,Yige Ma,Biao Shen,Xue Lv,Di Wang,Na An,Xiaoli Ma,Guangfeng Geng,Jingyuan Tong,Jinhua Liu,Guo Chen,Meng Gao,Ryo Kurita,Yukio Nakamura,Ping Zhu,Hang Yin,Xiaofan Zhu,Lihong Shi

Nature communications 15:3528 PubMed38664444

2024

BIN1 knockdown rescues systolic dysfunction in aging male mouse hearts.

Applications

Unspecified application

Species

Unspecified reactive species

Maartje Westhoff,Silvia G Del Villar,Taylor L Voelker,Phung N Thai,Heather C Spooner,Alexandre D Costa,Padmini Sirish,Nipavan Chiamvimonvat,Eamonn J Dickson,Rose E Dixon

Communications biology 7:301 PubMed38461223

2024

ADAMTS4 is a crucial proteolytic enzyme for versican cleavage in the amnion at parturition.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Die Li,Jiang-Wen Lu,Fan Zhang,Wen-Jia Lei,Fan Pan,Yi-Kai Lin,Li-Jun Ling,Leslie Myatt,Wang-Sheng Wang,Kang Sun

The Journal of experimental medicine 221: PubMed38442270

2024

NADPH oxidase exerts a B cell-intrinsic contribution to lupus risk by modulating endosomal TLR signals.

Applications

Unspecified application

Species

Unspecified reactive species

Shuozhi Liu,Jonathan Lagos,Natali M Shumlak,Andrea D Largent,Sebastien T E Lewis,Ursula Holder,Samuel W Du,Yifan Liu,Baidong Hou,Mridu Acharya,Shaun W Jackson

Cancer communications (London, England) 44:251-272 PubMed38152992

2023

NHE7 upregulation potentiates the uptake of small extracellular vesicles by enhancing maturation of macropinosomes in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Yao,Yi Xu,Liang Yu,Ting-Mao Xue,Zhi-Jie Xiao,Pui-Chi Tin,Hiu-Ling Fung,Hoi-Tang Ma,Jing-Ping Yun,Judy Wai Ping Yam

Molecular biology of the cell 35:ar27 PubMed38117589

2023

Cross-family small GTPase ubiquitination by the intracellular pathogen .

Applications

Unspecified application

Species

Unspecified reactive species

Adriana Steinbach,Varun Bhadkamkar,David Jimenez-Morales,Erica Stevenson,Gwendolyn M Jang,Nevan J Krogan,Danielle L Swaney,Shaeri Mukherjee
View all publications
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