AB137403

Anti-EEA1 antibody - C-terminal

Name: Anti-EEA1 antibody - C-terminal (ab137403) | Abcam
Brand: Abcam Limited
SKU: AB137403
Price: 545 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal EEA1 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human EEA1 aa 1300 to C-terminus.

View Alternative Names

ZFYVE2, EEA1, Early endosome antigen 1, Endosome-associated protein p162, Zinc finger FYVE domain-containing protein 2

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - C-terminal (AB137403)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - C-terminal (AB137403)

Confocal immunofluorescence analysis of methanol-fixed HeLa cells labelling EEA1 with ab137403 at 1/500 (Green). Alpha-tubulin filaments are labeled in Red.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - C-terminal (AB137403)

Immunofluorescence analysis of DIV9 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at RT for 15 min labelling EEA1 protein using ab137403 at a 1/500 dilution (Green). Beta Tubulin 3/ Tuj1 was counterstained in red. DAPI was used for nuclear staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEA1 antibody - C-terminal (AB137403)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEA1 antibody - C-terminal (AB137403)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue stained for EEA1 protein at cytosol on mouse brain with ab137403 at a 1/500 dilution.

Antigen Retrieval : (EDTA based, pH 8.0) buffer, 15min.

Unknown

Western blot - Anti-EEA1 antibody - C-terminal (AB137403)

5% SDS PAGE

All lanes:

Western blot - Anti-EEA1 antibody - C-terminal (ab137403) at 1/1000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 162 kDa

false

Supplier Data

Western blot - Anti-EEA1 antibody - C-terminal (AB137403)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-EEA1 antibody - C-terminal (ab137403) at 1/1000 dilution

Lane 1:

PC-12 whole cell lysate at 30 µg

Lane 2:

Rat2 whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 160 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human EEA1 aa 1300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q15075

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EEA1 (Early Endosome Antigen 1) is an important protein involved in endosomal trafficking with a molecular weight of approximately 162 kDa. It is a cytoplasmic protein expressed in various cell types acting mainly as an endosome marker. This protein contains a FYVE domain which facilitates binding to phosphatidylinositol 3-phosphate on endosomal membranes. EEA1 also interacts with Rab5 a small GTPase contributing to the fusion of early endosomes.

Biological function summary

EEA1 plays a significant role in early endosomal pathways facilitating endocytic vesicle fusion. EEA1 does not usually work alone; it functions as part of a larger complex involving other endosomal proteins. This protein acts as a tethering molecule which stabilizes the interaction of vesicles with endosomes. By anchoring vesicles EEA1 ensures the correct delivery and sorting of cargo within the cell which is important for cellular homeostasis and signaling.

Pathways

EEA1 contributes to the endocytic pathway and receptor recycling. It ensures proper functioning of the endocytic recycling of receptors such as the epidermal growth factor receptor (EGFR). EEA1-mediated tethering is vital for these pathways and it works closely with proteins like Rab5 which regulate endocytic trafficking. Through these interactions EEA1 affects cellular processes by controlling the flow of signaling receptors and other molecules within the cell.

EEA1 dysfunction is linked to neurodegenerative diseases and cancer. Abnormalities in its expression or function can disrupt endosomal trafficking leading to cellular stress and disease. For example in Alzheimer's disease impaired endosomal function involving EEA1 can contribute to amyloid-beta accumulation. In cancer altered EEA1 activity may contribute to aberrant receptor signaling which can promote unchecked cell division. The interplay with proteins like EGFR demonstrates EEA1's importance in maintaining cellular health and response mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.

See full target information EEA1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed38612903

2024

Characterization of the First Secreted Sorting Nexin Identified in the Protists.

Applications

Unspecified application

Species

Unspecified reactive species

Olympia Tziouvara,Marina Petsana,Drosos Kourounis,Amalia Papadaki,Efthimia Basdra,Georgia G Braliou,Haralabia Boleti

Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie 257:83-94 PubMed30367290

2018

Fate of the Fc fusion protein aflibercept in retinal endothelial cells: competition of recycling and degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Heidrun L Deissler,Gerhard K Lang,Gabriele E Lang

Oncotarget 9:6463-6477 PubMed29464085

2018

Intravesicular epidermal growth factor receptor subject to retrograde trafficking drives epidermal growth factor-dependent migration.

Applications

Unspecified application

Species

Unspecified reactive species

Sabrina Maisel,Derrick Broka,Joyce Schroeder

Experimental eye research 143:49-59 PubMed26481553

2015

Internalization of bevacizumab by retinal endothelial cells and its intracellular fate: Evidence for an involvement of the neonatal Fc receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Heidrun L Deissler,Gerhard K Lang,Gabriele E Lang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-EEA1 antibody - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - C-terminal (AB137403)

Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - C-terminal (AB137403)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEA1 antibody - C-terminal (AB137403)

Western blot - Anti-EEA1 antibody - C-terminal (AB137403)

Western blot - Anti-EEA1 antibody - C-terminal (AB137403)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise