Goat Polyclonal EEA1 antibody. Early Endosome marker. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human EEA1 aa 800-850.
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- Cell reports 40:1110622022Inhibition of SIRT2 promotes APP acetylation and ameliorates cognitive impairment in APP/PS1 transgenic mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNing Bai,Na Li,Rong Cheng,Yi Guan,Xiong Zhao,Zhijie Song,Hongde Xu,Fei Yi,Bo Jiang,Xiaoman Li,Xuan Wu,Cui Jiang,Tingting Zhou,Qiqiang Guo,Wendong Guo,Yanling Feng,Zhuo Wang,Mengtao Ma,Yang Yu,Zhanyou Wang,Shengping Zhang,Chuangui Wang,Weidong Zhao,Shihui Liu,Xiaoyu Song,Hua Liu,Liu CaoPubMed 35830807
- JHEP reports : innovation in hepatology 4:1003912021Immunomodulatory receptor VSIG4 is released during spontaneous bacterial peritonitis and predicts short-term mortality.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohanna Reißing,Philipp Lutz,Mick Frissen,Oluwatomi Ibidapo-Obe,Philipp A Reuken,Theresa H Wirtz,Sven Stengel,Stefanie Quickert,Michael Rooney,Karsten Große,Henning W Zimmermann,Christian Trautwein,Andreas Stallmach,Tony BrunsPubMed 34917912
- mSphere :e00217212021Cold Atmospheric Plasma Promotes Killing of Staphylococcus aureus by Macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesConstance Duchesne,Nadira Frescaline,Océane Blaise,Jean-Jacques Lataillade,Sébastien Banzet,Olivier Dussurget,Antoine RousseauPubMed 34133202
- Nucleic acids research 47:11020-110432019HER3-targeted protein chimera forms endosomolytic capsomeres and self-assembles into stealth nucleocapsids for systemic tumor homing of RNA interference in vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFelix Alonso-Valenteen,Sayuri Pacheco,Dustin Srinivas,Altan Rentsendorj,David Chu,Jay Lubow,Jessica Sims,Tianxin Miao,Simoun Mikhael,Jae Youn Hwang,Ravinder Abrol,Lali K Medina KauwePubMed 31617560
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human EEA1 aa 800-850. The exact immunogen used to generate this antibody is proprietary information. Database link Q15075
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Expected | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.30000-1.00000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
Alternative names
ZFYVE2, EEA1, Early endosome antigen 1, Endosome-associated protein p162, Zinc finger FYVE domain-containing protein 2
Recommended products
Goat Polyclonal EEA1 antibody. Early Endosome marker. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human EEA1 aa 800-850.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human EEA1 aa 800-850. The exact immunogen used to generate this antibody is proprietary information. Database link Q15075
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab206860 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EEA1 (Early Endosome Antigen 1) is an important protein involved in endosomal trafficking with a molecular weight of approximately 162 kDa. It is a cytoplasmic protein expressed in various cell types acting mainly as an endosome marker. This protein contains a FYVE domain which facilitates binding to phosphatidylinositol 3-phosphate on endosomal membranes. EEA1 also interacts with Rab5 a small GTPase contributing to the fusion of early endosomes.
- Biological function summary
EEA1 plays a significant role in early endosomal pathways facilitating endocytic vesicle fusion. EEA1 does not usually work alone; it functions as part of a larger complex involving other endosomal proteins. This protein acts as a tethering molecule which stabilizes the interaction of vesicles with endosomes. By anchoring vesicles EEA1 ensures the correct delivery and sorting of cargo within the cell which is important for cellular homeostasis and signaling.
- Pathways
EEA1 contributes to the endocytic pathway and receptor recycling. It ensures proper functioning of the endocytic recycling of receptors such as the epidermal growth factor receptor (EGFR). EEA1-mediated tethering is vital for these pathways and it works closely with proteins like Rab5 which regulate endocytic trafficking. Through these interactions EEA1 affects cellular processes by controlling the flow of signaling receptors and other molecules within the cell.
- Associated diseases and disorders
EEA1 dysfunction is linked to neurodegenerative diseases and cancer. Abnormalities in its expression or function can disrupt endosomal trafficking leading to cellular stress and disease. For example in Alzheimer's disease impaired endosomal function involving EEA1 can contribute to amyloid-beta accumulation. In cancer altered EEA1 activity may contribute to aberrant receptor signaling which can promote unchecked cell division. The interplay with proteins like EGFR demonstrates EEA1's importance in maintaining cellular health and response mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - Early Endosome Marker (ab206860)
Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells permeabilized with 0.15% Triton staining EEA1. Primary incubation with ab206860 (5µg/ml) for 1 hour followed by Alexa Fluor 488 secondary antibody (2µg/ml). Nuclear counter stain is DAPI.
Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab206860)
All lanes: Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab206860) at 0.3 µg/mL
All lanes: HEK293 lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 162 kDa
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