AB206860

Anti-EEA1 antibody - Early Endosome Marker

Name: Anti-EEA1 antibody - Early Endosome Marker (ab206860) | Abcam
Brand: Abcam Limited
SKU: AB206860
Price: 400 USD
Availability: InStock
Rating: 5 (1 reviews)

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What is this?

(1 Review)

(8 Publications)

Goat Polyclonal EEA1 antibody. Early Endosome marker. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human EEA1 aa 800-850.

View Alternative Names

ZFYVE2, EEA1, Early endosome antigen 1, Endosome-associated protein p162, Zinc finger FYVE domain-containing protein 2

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - Early Endosome Marker (AB206860)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - Early Endosome Marker (AB206860)

Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells permeabilized with 0.15% Triton staining EEA1. Primary incubation with ab206860 (5µg/ml) for 1 hour followed by Alexa Fluor 488 secondary antibody (2µg/ml). Nuclear counter stain is DAPI.

Supplier Data

Western blot - Anti-EEA1 antibody - Early Endosome Marker (AB206860)

All lanes:

Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab206860) at 0.3 µg/mL

All lanes:

HEK293 lysate (in RIPA buffer) at 35 µg

Predicted band size: 162 kDa

true

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human EEA1 aa 800-850. The exact immunogen used to generate this antibody is proprietary information.

Q15075

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab206860 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EEA1 (Early Endosome Antigen 1) is an important protein involved in endosomal trafficking with a molecular weight of approximately 162 kDa. It is a cytoplasmic protein expressed in various cell types acting mainly as an endosome marker. This protein contains a FYVE domain which facilitates binding to phosphatidylinositol 3-phosphate on endosomal membranes. EEA1 also interacts with Rab5 a small GTPase contributing to the fusion of early endosomes.

Biological function summary

EEA1 plays a significant role in early endosomal pathways facilitating endocytic vesicle fusion. EEA1 does not usually work alone; it functions as part of a larger complex involving other endosomal proteins. This protein acts as a tethering molecule which stabilizes the interaction of vesicles with endosomes. By anchoring vesicles EEA1 ensures the correct delivery and sorting of cargo within the cell which is important for cellular homeostasis and signaling.

Pathways

EEA1 contributes to the endocytic pathway and receptor recycling. It ensures proper functioning of the endocytic recycling of receptors such as the epidermal growth factor receptor (EGFR). EEA1-mediated tethering is vital for these pathways and it works closely with proteins like Rab5 which regulate endocytic trafficking. Through these interactions EEA1 affects cellular processes by controlling the flow of signaling receptors and other molecules within the cell.

EEA1 dysfunction is linked to neurodegenerative diseases and cancer. Abnormalities in its expression or function can disrupt endosomal trafficking leading to cellular stress and disease. For example in Alzheimer's disease impaired endosomal function involving EEA1 can contribute to amyloid-beta accumulation. In cancer altered EEA1 activity may contribute to aberrant receptor signaling which can promote unchecked cell division. The interplay with proteins like EGFR demonstrates EEA1's importance in maintaining cellular health and response mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.

See full target information EEA1

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Nature nanotechnology 20:683-696 PubMed39984637

2025

Systemic HER3 ligand-mimicking nanobioparticles enter the brain and reduce intracranial tumour growth.

Applications

Unspecified application

Species

Unspecified reactive species

Felix Alonso-Valenteen,Simoun Mikhael,HongQiang Wang,Jessica Sims,Michael Taguiam,James Teh,Sam Sances,Michelle Wong,Tianxin Miao,Dustin Srinivas,Nelyda Gonzalez-Almeyda,Ryan H Cho,Romny Sanchez,Kimngan Nguyenle,Erik Serrano,Briana Ondatje,Rebecca L Benhaghnazar,Harry B Gray,Zeev Gross,John Yu,Clive N Svendsen,Ravinder Abrol,Lali K Medina-Kauwe

International journal of molecular sciences 25: PubMed39769151

2025

Keratinocyte-Mediated Antigen Presentation in Psoriasis: Preliminary Insights from In Vitro Studies.

Applications

Unspecified application

Species

Unspecified reactive species

Katarzyna Zima,Dorota Purzycka-Bohdan,Aneta Szczerkowska-Dobosz,Magdalena Gabig-Cimińska

Cell reports 40:111062 PubMed35830807

2022

Inhibition of SIRT2 promotes APP acetylation and ameliorates cognitive impairment in APP/PS1 transgenic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Bai,Na Li,Rong Cheng,Yi Guan,Xiong Zhao,Zhijie Song,Hongde Xu,Fei Yi,Bo Jiang,Xiaoman Li,Xuan Wu,Cui Jiang,Tingting Zhou,Qiqiang Guo,Wendong Guo,Yanling Feng,Zhuo Wang,Mengtao Ma,Yang Yu,Zhanyou Wang,Shengping Zhang,Chuangui Wang,Weidong Zhao,Shihui Liu,Xiaoyu Song,Hua Liu,Liu Cao

JHEP reports : innovation in hepatology 4:100391 PubMed34917912

2021

Immunomodulatory receptor VSIG4 is released during spontaneous bacterial peritonitis and predicts short-term mortality.

Applications

Unspecified application

Species

Unspecified reactive species

Johanna Reißing,Philipp Lutz,Mick Frissen,Oluwatomi Ibidapo-Obe,Philipp A Reuken,Theresa H Wirtz,Sven Stengel,Stefanie Quickert,Michael Rooney,Karsten Große,Henning W Zimmermann,Christian Trautwein,Andreas Stallmach,Tony Bruns

Advanced healthcare materials 11:e2102027 PubMed34865307

2021

Harnessing the Natural Healing Power of Colostrum: Bovine Milk-Derived Extracellular Vesicles from Colostrum Facilitating the Transition from Inflammation to Tissue Regeneration for Accelerating Cutaneous Wound Healing.

Applications

Unspecified application

Species

Unspecified reactive species

Hyosuk Kim,Da Eun Kim,Geonhee Han,Nu Ri Lim,Eun Hye Kim,Yeongji Jang,Haeun Cho,Hochung Jang,Ki Hun Kim,Sun Hwa Kim,Yoosoo Yang

Frontiers in pharmacology 12:710722 PubMed34603025

2021

Intervention of Gastrodin in Type 2 Diabetes Mellitus and Its Mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Bai,Ke Mo,Guirong Wang,Wanling Chen,Wei Zhang,Yibo Guo,Zhirong Sun

mSphere :e0021721 PubMed34133202

2021

Cold Atmospheric Plasma Promotes Killing of Staphylococcus aureus by Macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Constance Duchesne,Nadira Frescaline,Océane Blaise,Jean-Jacques Lataillade,Sébastien Banzet,Olivier Dussurget,Antoine Rousseau

Nucleic acids research 47:11020-11043 PubMed31617560

2019

HER3-targeted protein chimera forms endosomolytic capsomeres and self-assembles into stealth nucleocapsids for systemic tumor homing of RNA interference in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Felix Alonso-Valenteen,Sayuri Pacheco,Dustin Srinivas,Altan Rentsendorj,David Chu,Jay Lubow,Jessica Sims,Tianxin Miao,Simoun Mikhael,Jae Youn Hwang,Ravinder Abrol,Lali K Medina Kauwe

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EEA1 antibody - Early Endosome Marker

Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - Early Endosome Marker (AB206860)

Western blot - Anti-EEA1 antibody - Early Endosome Marker (AB206860)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

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