Anti-EEA1 antibody is a rabbit polyclonal antibody that is used in early endosome antigen 1 western blot (WB) and immunocytochemistry/immunofluorescence (ICC/IF). Suitable for human, mouse, rat, and more samples.
- Specificity confirmed with EEA1 knockout cell line validation
- Cited in over 250 publications
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- PLoS pathogens 19:e10118872023Roles of epidermal growth factor receptor, claudin-1 and occludin in multi-step entry of hepatitis C virus into polarized hepatoma spheroids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChui-Wa So,Marion Sourisseau,Shamila Sarwar,Matthew J Evans,Glenn RandallPubMed 38157366
- Aging cell 22:e139732023Degradation of cochlear Connexin26 accelerate the development of age-related hearing loss.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKai Xu,Sen Chen,Xue Bai,Le Xie,Yue Qiu,Xiao-Zhou Liu,Xiao-Hui Wang,Wei-Jia Kong,Yu SunPubMed 37681746
- Frontiers in aging neuroscience 15:11174912023Uric acid regulates α-synuclein transmission in Parkinsonian models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Jin Shin,Yeon Ju Kim,Ji Eun Lee,Yi Seul Kim,Jung Wook Lee,HyeonJeong Kim,Jin Young Shin,Phil Hyu LeePubMed 37711993
- Small (Weinheim an der Bergstrasse, Germany) 19:e22042382022One Stone, Two Birds: A Peptide-Au(I) Infinite Coordination Supermolecule for the Confederate Physical and Biological Radiosensitization in Cancer Radiation Therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Wang,Jing Zhao,Fang Ma,Liuyun Gong,Yinliang Lu,Weiping Xiao,Hanmin Tang,Chengyi Gao,Yuetong Chen,Jun Ma,Zhan Gao,Jin Yan,Suxia HanPubMed 36494177
- Translational psychiatry 12:5082022Defective proteostasis in induced pluripotent stem cell models of frontotemporal lobar degeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSidhartha Mahali,Rita Martinez,Melvin King,Anthony Verbeck,Oscar Harari,Bruno A Benitez,Kanta Horie,Chihiro Sato,Sally Temple,Celeste M KarchPubMed 36494352
- Journal of virology 97:e01331222022Oropouche Virus Glycoprotein Topology and Cellular Requirements for Glycoprotein Secretion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNatalia S Barbosa,Juan O Concha,Luis L P daSilva,Colin M Crump,Stephen C GrahamPubMed 36475765
- Protein science : a publication of the Protein Society 31:e44472022Evolutionary trajectory of receptor binding specificity and promiscuity of the spike protein of SARS-CoV-2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCyril Planchais,Alejandra Reyes-Ruiz,Robin Lacombe,Alessandra Zarantonello,Maxime Lecerf,Margot Revel,Lubka T Roumenina,Boris P Atanasov,Hugo Mouquet,Jordan D DimitrovPubMed 36305765
- Cellular and molecular life sciences : CMLS 79:5662022In human astrocytes neurotropic flaviviruses increase autophagy, yet their replication is autophagy-independent.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPetra Tavčar Verdev,Maja Potokar,Miša Korva,Katarina Resman Rus,Marko Kolenc,Tatjana Avšič Županc,Robert Zorec,Jernej JorgačevskiPubMed 36283999
- Nature neuroscience 25:1273-12782022Oligodendrocyte precursor cells engulf synapses during circuit remodeling in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYohan S S Auguste,Austin Ferro,Jessica A Kahng,Andre M Xavier,Jessica R Dixon,Uma Vrudhula,Anne-Sarah Nichitiu,Daniele Rosado,Tse-Luen Wee,Ullas V Pedmale,Lucas CheadlePubMed 36171430
- Biology open 11:2022The uncoating of EV71 in mature late endosomes requires CD-M6PR.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeii Ohka,Soon Hao Tan,Eri Ishiyama,Katsutoshi Ogasawara,Tomohito Hanasaka,Kinji Ishida,Kyoji Hagiwara,Chia-Chyi Liu,Pele Choi-Sing Chong,Ken-Ichi Hanaki,Giampietro SchiavoPubMed 35929543
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Expected | Expected |
| Aplysia | Predicted | Predicted |
| Chicken | Predicted | Predicted |
| Chinese hamster | Expected | Expected |
| Cow | Predicted | Predicted |
| Dog | Expected | Expected |
| Hamster | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
| Xenopus laevis | Expected | Expected |
| Zebrafish | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes Abcam recommends using milk as the blocking agent. |
Species Human | Dilution info 1 µg/mL | Notes Abcam recommends using milk as the blocking agent. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chinese hamster | Dilution info 1 µg/mL | Notes Abcam recommends using milk as the blocking agent. |
Species Rat | Dilution info 1 µg/mL | Notes Abcam recommends using milk as the blocking agent. |
Species Xenopus laevis | Dilution info 1 µg/mL | Notes Abcam recommends using milk as the blocking agent. |
Species Dog | Dilution info 1 µg/mL | Notes Abcam recommends using milk as the blocking agent. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Hamster, Cow, Zebrafish, Rhesus monkey, Aplysia | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-5.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1.00000-5.00000 µg/mL | Notes - |
Species Mouse | Dilution info 1.00000-5.00000 µg/mL | Notes - |
Species Chinese hamster | Dilution info 1.00000-5.00000 µg/mL | Notes - |
Species Dog | Dilution info 1.00000-5.00000 µg/mL | Notes - |
Species Xenopus laevis | Dilution info 1.00000-5.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Hamster, Cow, Zebrafish, Rhesus monkey, Aplysia | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
Alternative names
ZFYVE2, EEA1, Early endosome antigen 1, Endosome-associated protein p162, Zinc finger FYVE domain-containing protein 2
Recommended products
Anti-EEA1 antibody is a rabbit polyclonal antibody that is used in early endosome antigen 1 western blot (WB) and immunocytochemistry/immunofluorescence (ICC/IF). Suitable for human, mouse, rat, and more samples.
- Specificity confirmed with EEA1 knockout cell line validation
- Cited in over 250 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
Detects a band at 180kDa that represents EEA1 in Western blotting on human cell lines (corresponds to results seen in Mu et al). Also detects a band at 100kDa, we are unsure as to the identity of this band. Immunofluorescence staining of EEA1 in HeLa cells yields a punctate staining pattern consistent with the cytoplasmic distribution of endosomes. From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab109110.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Product Specifications
Anti-EEA1 antibody - Early Endosome Marker (ab2900) is a rabbit polyclonal antibody and is validated for use in ICC/IF, WB in chinese hamster, dog, human, mouse, rat, xenopus laevis samples.
Anti-EEA1 antibody - Early Endosome Marker (ab2900) specifically detects EEA1 (UniProt ID: Q15075; Molecular weight: 162kDa) and is sold in 100 µg selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-EEA1 antibody - Early Endosome Marker (ab2900) has high sensitivity and specificity.
The specificity of Anti-EEA1 antibody - Early Endosome Marker (ab2900) has been confirmed by testing in knockout samples.
Anti-EEA1 antibody - Early Endosome Marker (ab2900) has been cited over 251 times in peer reviewed journals and is trusted by the scientific community.
Anti-EEA1 antibody - Early Endosome Marker (ab2900) has 39 independent reviews from customers.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EEA1 (Early Endosome Antigen 1) is an important protein involved in endosomal trafficking with a molecular weight of approximately 162 kDa. It is a cytoplasmic protein expressed in various cell types acting mainly as an endosome marker. This protein contains a FYVE domain which facilitates binding to phosphatidylinositol 3-phosphate on endosomal membranes. EEA1 also interacts with Rab5 a small GTPase contributing to the fusion of early endosomes.
- Biological function summary
EEA1 plays a significant role in early endosomal pathways facilitating endocytic vesicle fusion. EEA1 does not usually work alone; it functions as part of a larger complex involving other endosomal proteins. This protein acts as a tethering molecule which stabilizes the interaction of vesicles with endosomes. By anchoring vesicles EEA1 ensures the correct delivery and sorting of cargo within the cell which is important for cellular homeostasis and signaling.
- Pathways
EEA1 contributes to the endocytic pathway and receptor recycling. It ensures proper functioning of the endocytic recycling of receptors such as the epidermal growth factor receptor (EGFR). EEA1-mediated tethering is vital for these pathways and it works closely with proteins like Rab5 which regulate endocytic trafficking. Through these interactions EEA1 affects cellular processes by controlling the flow of signaling receptors and other molecules within the cell.
- Associated diseases and disorders
EEA1 dysfunction is linked to neurodegenerative diseases and cancer. Abnormalities in its expression or function can disrupt endosomal trafficking leading to cellular stress and disease. For example in Alzheimer's disease impaired endosomal function involving EEA1 can contribute to amyloid-beta accumulation. In cancer altered EEA1 activity may contribute to aberrant receptor signaling which can promote unchecked cell division. The interplay with proteins like EGFR demonstrates EEA1's importance in maintaining cellular health and response mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
ab2900 staining EEA1 in HepG2 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab2900 at 5µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
Lane 2: EEA1 knockout HAP1 whole cell lysate (20 μg)
Lane 3: HeLa whole cell lysate (20 μg)
Lane 4: NIH3T3 whole cell lysate (20 μg)Lanes 1 - 4: Merged signal (red and green). Green - ab2900 observed at 162 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab2900 was shown to recognize EEA1 in wild-type HAP1 cells as signal was lost at the expected MW in EEA1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and EEA1 knockout samples were subjected to SDS-PAGE. ab2900 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
Predicted band size: 162 kDa
Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin (Lanes 1-3) or 3% Milk (Lanes 4-6) before being incubated with ab2900 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
All lanes: Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900) at 1 µg/mL
Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 2: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
Lane 3: NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 20 µg
Lane 4: HeLa whole cell lysate at 20 µg
Lane 5: HEK-293 whole cell lysate at 20 µg
Lane 6: NIH 3T3 whole cell lysate at 20 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG H&L (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 162 kDa
Observed band size: 100 kDa, 180 kDa
Exposure time: 30s
Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
Fluorescence detection of secondary antibody.
All lanes: Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900) at 1/1000 dilution
Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) nuclear lysate at 20 µg
Lane 2: HeLa whole cell lysate at 20 µg
Lane 3: A431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg
Lane 4: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg
Lane 5: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
SecondaryAll lanes: Alexa Fluor anti rabbit at 1/50000 dilution
Performed under reducing conditions.
Predicted band size: 162 kDa
Observed band size: 100 kDa, 180 kDa, 41 kDa, 50 kDa
This image is courtesy of Randal Moldrich, CNRS UMR7637, ESPCI, FranceImmunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
Confocal microscopy of fixed primary cultures of rat hippocampal neurons (embryonic day 18) showing immunocytochemical labelling of rabbit polyclonal to EEA1 (ab2900, 1/200; Alexa Fluor 488 1/200; green) and monoclonal mouse anti-β.
This image is courtesy of an anonymous customer reviewImmunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
ab2900 staining mouse L-cells by ICC/IF. Cells were formaldehyde fixed, permeabilized in Triton X-100 and incubated with ab2900 diluted 1/1000 for 1 hour at 37°C. A Cy2® conjugated goat anti-rabbit antibody was used as the secondary.
Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
Lane 1 - 2 : EEA1 antibody - Early Endosome Marker (ab2900) at 1/500 dilution, HEK293 Whole Cell lysate at 20 ug
Lane 1 : as above
Lane 2 : EEA1 peptide (ab14946) at 1 ug
Secondary
Goat polyclonal to Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab6721) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 160kD
Observed band size : 180kD (why is the actual band size different from the predicted?)All lanes: Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900) at 1/500 dilution
Lane 1: HEK293 Whole Cell lysate at 20 µg
Lane 2: HEK293 Whole Cell lysate at 20 µg with Human EEA1 peptide (ab14946)
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab6721) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 162 kDa
Observed band size: 180 kDa
Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
The Western blot shows that ab14944 reacts strongly with mouse 3T3 and CHO cell lysates. Weak cross-reactivity is seen with Xenopus, mouse brain, mouse liver, rat brain and dog lysates. The antibody does not appear to cross-react with rat liver lysate.
All lanes: Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900) at 1 µg/mL
Lane 1: Xenopus laevis lysate at 20 µg
Lane 2: Mouse 3T3 cell lysate at 20 µg
Lane 3: Mouse brain cell lysate at 20 µg
Lane 4: Mouse liver cell lysate at 20 µg
Lane 5: Rat brain cell lysate at 20 µg
Lane 6: Rat liver cell lysate at 20 µg
Lane 7: Dog lysate at 20 µg
Lane 8: CHO cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab6721) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 162 kDa
Observed band size: 100 kDa, 180 kDa
Exposure time: 30s
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