Rabbit Recombinant Monoclonal EEA1 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Mouse, Human, Rat, African green monkey samples.
Images
![Western blot - Anti-EEA1 antibody [EPR4245] - BSA and Azide free (AB239942), expandable thumbnail](https://content.abcam.com/products/images/eea1-antibody-epr4245-bsa-and-azide-free-ab239942--western-blot-img139339.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-EEA1 antibody [EPR4245] - BSA and Azide free (AB239942)")
![Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [EPR4245] - BSA and Azide free (AB239942), expandable thumbnail](https://content.abcam.com/products/images/eea1-antibody-epr4245-bsa-and-azide-free-ab239942--immunocytochemistry-immunofluorescence-img69909.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [EPR4245] - BSA and Azide free (AB239942)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | |
|---|---|---|---|
| Human | Not recommended | Tested | Tested |
| Mouse | Not recommended | Tested | Expected |
| Rat | Not recommended | Tested | Expected |
| African green monkey | Not recommended | Tested | Expected |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, African green monkey, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat, African green monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, African green monkey | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
3 products for Alternative Version
Target data
Function
Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
Alternative names
ZFYVE2, EEA1, Early endosome antigen 1, Endosome-associated protein p162, Zinc finger FYVE domain-containing protein 2
Recommended products
Rabbit Recombinant Monoclonal EEA1 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Mouse, Human, Rat, African green monkey samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR4245
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab239942 is the carrier-free version of Anti-EEA1 antibody [EPR4245] - Early Endosome Marker ab109110.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EEA1 (Early Endosome Antigen 1) is an important protein involved in endosomal trafficking with a molecular weight of approximately 162 kDa. It is a cytoplasmic protein expressed in various cell types acting mainly as an endosome marker. This protein contains a FYVE domain which facilitates binding to phosphatidylinositol 3-phosphate on endosomal membranes. EEA1 also interacts with Rab5 a small GTPase contributing to the fusion of early endosomes.
- Biological function summary
EEA1 plays a significant role in early endosomal pathways facilitating endocytic vesicle fusion. EEA1 does not usually work alone; it functions as part of a larger complex involving other endosomal proteins. This protein acts as a tethering molecule which stabilizes the interaction of vesicles with endosomes. By anchoring vesicles EEA1 ensures the correct delivery and sorting of cargo within the cell which is important for cellular homeostasis and signaling.
- Pathways
EEA1 contributes to the endocytic pathway and receptor recycling. It ensures proper functioning of the endocytic recycling of receptors such as the epidermal growth factor receptor (EGFR). EEA1-mediated tethering is vital for these pathways and it works closely with proteins like Rab5 which regulate endocytic trafficking. Through these interactions EEA1 affects cellular processes by controlling the flow of signaling receptors and other molecules within the cell.
- Associated diseases and disorders
EEA1 dysfunction is linked to neurodegenerative diseases and cancer. Abnormalities in its expression or function can disrupt endosomal trafficking leading to cellular stress and disease. For example in Alzheimer's disease impaired endosomal function involving EEA1 can contribute to amyloid-beta accumulation. In cancer altered EEA1 activity may contribute to aberrant receptor signaling which can promote unchecked cell division. The interplay with proteins like EGFR demonstrates EEA1's importance in maintaining cellular health and response mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-EEA1 antibody [EPR4245] - BSA and Azide free (ab239942), expandable thumbnail](https://content.abcam.com/products/images/eea1-antibody-epr4245-bsa-and-azide-free-ab239942--western-blot-img139339.jpg "Western blot - Anti-EEA1 antibody [EPR4245] - BSA and Azide free (ab239942)")
Western blot - Anti-EEA1 antibody [EPR4245] - BSA and Azide free (ab239942)
This data was developed using the same antibody clone in a different buffer formulation (Anti-EEA1 antibody [EPR4245] - Early Endosome Marker ab109110).
Lanes 1- 4: Merged signal (red and green). Green - Anti-EEA1 antibody [EPR4245] - Early Endosome Marker ab109110 observed at 175 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.
Anti-EEA1 antibody [EPR4245] - Early Endosome Marker ab109110 was shown to react with EEA1 in wild-type HeLa cells in western blot. The band observed in CRISPR/Cas9 editedcell line Human EEA1 knockout HeLa cell line ab261822 (CRISPR/Cas9 edited cell lysate Human EEA1 knockout HeLa cell lysate ab256897) lane below 175kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and EEA1 CRISPR/Cas9 edited HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-EEA1 antibody [EPR4245] - Early Endosome Marker ab109110 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (Anti-EEA1 antibody [EPR4245] - Early Endosome Marker ab109110) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: EEA1 CRISPR/Cas9 edited HeLa cell lysate at 20 µg
Lane 2: Western blot - Human EEA1 knockout HeLa cell line (Human EEA1 knockout HeLa cell line ab261822)
Lane 3: Daudi cell lysate at 20 µg
Lane 4: SH-SY5Y cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 162 kDa
Observed band size: 175 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [EPR4245] - BSA and Azide free (ab239942), expandable thumbnail](https://content.abcam.com/products/images/eea1-antibody-epr4245-bsa-and-azide-free-ab239942--immunocytochemistry-immunofluorescence-img69909.jpg "Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [EPR4245] - BSA and Azide free (ab239942)")
Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [EPR4245] - BSA and Azide free (ab239942)
Immunocytochemistry/Immunofluorescence analysis of JAR (human placenta choriocarcinoma epithelial) cells labelling EEA1 with Anti-EEA1 antibody [EPR4245] - Early Endosome Marker ab109110 at a dilution of 1/250. Cells were fixed with 4% paraformaldehye and permeabilized with 0.1% TritonX-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody at a dilution of 1/1000. Counterstained with DAPI and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594), at a dilution of 1/200.
Image shows cytoplasmic staining in JAR cell line.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-EEA1 antibody [EPR4245] - Early Endosome Marker ab109110).
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