Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110) is a rabbit monoclonal antibody detecting EEA1 in Western Blot, ICC/IF. Suitable for African green monkey, Human, Mouse, Rat.
- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications
Images
![Western blot - Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (AB109110), expandable thumbnail](https://content.abcam.com/products/images/eea1-antibody-epr4245-early-endosome-marker-ab109110--western-blot-img139339.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (AB109110)")
![Western blot - Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (AB109110), expandable thumbnail](https://content.abcam.com/products/images/eea1-antibody-epr4245-early-endosome-marker-ab109110--western-blot-img152299.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (AB109110)")
![Western blot - Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (AB109110), expandable thumbnail](https://content.abcam.com/products/images/eea1-antibody-epr4245-early-endosome-marker-ab109110--western-blot-img13702.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (AB109110)")
![Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (AB109110), expandable thumbnail](https://content.abcam.com/products/images/eea1-antibody-epr4245-early-endosome-marker-ab109110--immunocytochemistry-immunofluorescence-img69909.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (AB109110)")
![Western blot - Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (AB109110), expandable thumbnail](https://content.abcam.com/products/images/eea1-antibody-epr4245-early-endosome-marker-ab109110--western-blot-img112166.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (AB109110)")
Publications
Filter by
- Fluids and barriers of the CNS 20:822023Subcellular trafficking and transcytosis efficacy of different receptor types for therapeutic antibody delivery at the blood‒brain barrier.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMikkel Roland Holst,Nienke Marije de Wit,Burak Ozgür,Andreas Brachner,Kathrine Hyldig,Antje Appelt-Menzel,Hannah Sleven,Zameel Cader,Helga Eveline de Vries,Winfried Neuhaus,Allan Jensen,Birger Brodin,Morten Schallburg NielsenPubMed 37932749
- Immunology 166:380-4072022Therapeutic antibody glycosylation impacts antigen recognition and immunogenicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBabette Wolf,Mateusz Piksa,Isabelle Beley,Agnes Patoux,Thierry Besson,Valerie Cordier,Bernd Voedisch,Patrick Schindler,Daniela Stöllner,Ludovic Perrot,Stephan von Gunten,Dominique Brees,Michael KammüllerPubMed 35416297
- Communications biology 5:2122022A NanoBiT assay to monitor membrane proteins trafficking for drug discovery and drug development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArfaxad Reyes-Alcaraz,Emilio Y Lucero Garcia-Rojas,Elizabeth A Merlinsky,Jae Young Seong,Richard A Bond,Bradley K McConnellPubMed 35260793
- Nanomaterials (Basel, Switzerland) 12:2022Cellular Uptake of Silica and Gold Nanoparticles Induces Early Activation of Nuclear Receptor NR4A1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMauro Sousa de Almeida,Patricia Taladriz-Blanco,Barbara Drasler,Sandor Balog,Phattadon Yajan,Alke Petri-Fink,Barbara Rothen-RutishauserPubMed 35215018
- Neuroscience bulletin 38:290-3022021Polysaccharide Krestin Prevents Alzheimer's Disease-type Pathology and Cognitive Deficits by Enhancing Monocyte Amyloid-β Processing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSi-Han Chen,Chen-Yang He,Ying-Ying Shen,Gui-Hua Zeng,Ding-Yuan Tian,Yuan Cheng,Man-Yu Xu,Dong-Yu Fan,Cheng-Rong Tan,An-Yu Shi,Xian-Le Bu,Yan-Jiang WangPubMed 34611829
- Cell reports 35:1092592021SORL1 deficiency in human excitatory neurons causes APP-dependent defects in the endolysosome-autophagy network.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristy Hung,Eleanor Tuck,Victoria Stubbs,Sven J van der Lee,Cora Aalfs,Resie van Spaendonk,Philip Scheltens,John Hardy,Henne Holstege,Frederick J LiveseyPubMed 34133918
- The Journal of biological chemistry 297:1008732021Synaptojanin1 deficiency upregulates basal autophagosome formation in astrocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPing-Yue Pan,Justin Zhu,Asma Rizvi,Xinyu Zhu,Hikari Tanaka,Cheryl F DreyfusPubMed 34126070
- Nature communications 11:59192020Regulation of cytokine signaling through direct interaction between cytokine receptors and the ATG16L1 WD40 domain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesInmaculada Serramito-Gómez et. al.PubMed 33219218
- Frontiers in immunology 11:5618432020Theta-Defensins Inhibit High-Risk Human Papillomavirus Infection Through Charge-Driven Capsid Clustering.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoseph G Skeate et. al.PubMed 33154746
- Scientific reports 10:153852020Cellular uptake of extracellular nucleosomes induces innate immune responses by binding and activating cGMP-AMP synthase (cGAS).Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuawei Wang et. al.PubMed 32958884
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | |
|---|---|---|---|
| Human | Not recommended | Tested | Tested |
| Mouse | Not recommended | Tested | Expected |
| Rat | Not recommended | Tested | Expected |
| African green monkey | Not recommended | Tested | Expected |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, African green monkey, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/10000 - 1/50000 | Notes - |
Species African green monkey | Dilution info 1/10000 - 1/50000 | Notes - |
Species Human | Dilution info 1/10000 - 1/50000 | Notes - |
Species Rat | Dilution info 1/10000 - 1/50000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, African green monkey, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
Alternative names
ZFYVE2, EEA1, Early endosome antigen 1, Endosome-associated protein p162, Zinc finger FYVE domain-containing protein 2
Recommended products
Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110) is a rabbit monoclonal antibody detecting EEA1 in Western Blot, ICC/IF. Suitable for African green monkey, Human, Mouse, Rat.
- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR4245
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Stable for 12 months at -20°C
Notes
Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in African green monkey, Human, Mouse, Rat samples.
What is the molecular weight of EEA1?
Anti-EEA1 [EPR4245] - Early Endosome Marker (ab109110) specifically detects a band for EEA1 (UniProt: Q15075) at a molecular weight of 162kDa.
Trusted by the scientific community
Anti-EEA1 [EPR4245] - Early Endosome Marker (ab109110) was first used in a scientific publication in 2011 and has been cited over 10 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Specificity confirmed
The specificity of Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110) has been confirmed by Western blot testing in EEA1 Knockout HAP1 cells.
Other related products
We have a range of other formats of antibody clone [EPR4245] also available for your convenience:
ab109110, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-EEA1 antibody [EPR4245] - Early Endosome Marker ab185039, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-EEA1 antibody [EPR4245] - Early Endosome Marker ab196186, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-EEA1 antibody [EPR4245] - Early Endosome Marker ab206913, Carrier free - Anti-EEA1 antibody [EPR4245] - BSA and Azide free ab239942
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EEA1 (Early Endosome Antigen 1) is an important protein involved in endosomal trafficking with a molecular weight of approximately 162 kDa. It is a cytoplasmic protein expressed in various cell types acting mainly as an endosome marker. This protein contains a FYVE domain which facilitates binding to phosphatidylinositol 3-phosphate on endosomal membranes. EEA1 also interacts with Rab5 a small GTPase contributing to the fusion of early endosomes.
- Biological function summary
EEA1 plays a significant role in early endosomal pathways facilitating endocytic vesicle fusion. EEA1 does not usually work alone; it functions as part of a larger complex involving other endosomal proteins. This protein acts as a tethering molecule which stabilizes the interaction of vesicles with endosomes. By anchoring vesicles EEA1 ensures the correct delivery and sorting of cargo within the cell which is important for cellular homeostasis and signaling.
- Pathways
EEA1 contributes to the endocytic pathway and receptor recycling. It ensures proper functioning of the endocytic recycling of receptors such as the epidermal growth factor receptor (EGFR). EEA1-mediated tethering is vital for these pathways and it works closely with proteins like Rab5 which regulate endocytic trafficking. Through these interactions EEA1 affects cellular processes by controlling the flow of signaling receptors and other molecules within the cell.
- Associated diseases and disorders
EEA1 dysfunction is linked to neurodegenerative diseases and cancer. Abnormalities in its expression or function can disrupt endosomal trafficking leading to cellular stress and disease. For example in Alzheimer's disease impaired endosomal function involving EEA1 can contribute to amyloid-beta accumulation. In cancer altered EEA1 activity may contribute to aberrant receptor signaling which can promote unchecked cell division. The interplay with proteins like EGFR demonstrates EEA1's importance in maintaining cellular health and response mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110), expandable thumbnail](https://content.abcam.com/products/images/eea1-antibody-epr4245-early-endosome-marker-ab109110--western-blot-img139339.jpg "Western blot - Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110)")
Western blot - Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110)
Lanes 1- 4: Merged signal (red and green). Green - ab109110 observed at 175 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.ab109110 was shown to react with EEA1 in wild-type HeLa cells in western blot. The band observed in CRISPR/Cas9 edited cell line Human EEA1 knockout HeLa cell line ab261822 (CRISPR/Cas9 edited cell lysate Human EEA1 knockout HeLa cell lysate ab256897) lane below 175kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and EEA1 CRISPR/Cas9 edited HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109110 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: EEA1 CRISPR/Cas9 edited HeLa cell lysate at 20 µg
Lane 2: Western blot - Human EEA1 knockout HeLa cell line (Human EEA1 knockout HeLa cell line ab261822)
Lane 3: Daudi cell lysate at 20 µg
Lane 4: SH-SY5Y cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 162 kDa
Observed band size: 175 kDa
![Western blot - Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110), expandable thumbnail](https://content.abcam.com/products/images/eea1-antibody-epr4245-early-endosome-marker-ab109110--western-blot-img152299.jpg "Western blot - Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110)")
Western blot - Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110)
Lanes 1- 4: Merged signal (red and green). Green - ab109110 observed at 175 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.ab109110 was shown to react with EEA1 in wild-type HeLa cells in western blot. The band observed in knockout cell line Human EEA1 knockout HeLa cell line ab261822 (knockout cell lysate Human EEA1 knockout HeLa cell lysate ab256897) lane below 175kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and EEA1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109110 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: EEA1 knockout HeLa cell lysate at 20 µg
Lane 3: Daudi cell lysate at 20 µg
Lane 4: SH-SY5Y cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 162 kDa
Observed band size: 175 kDa
![Western blot - Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110), expandable thumbnail](https://content.abcam.com/products/images/eea1-antibody-epr4245-early-endosome-marker-ab109110--western-blot-img13702.jpg "Western blot - Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110)")
Western blot - Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110)
Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
Lane 2: Early Endosome Marker knockout HAP1 whole cell lysate (20 μg)
Lane 3: HeLa whole cell lysate (20 μg)
Lane 4: NIH3T3 whole cell lysate (20 μg)Lanes 1 - 4: Merged signal (red and green). Green - ab109110 observed at 162 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab109110 was shown to recognize Early Endosome Marker in wild-type HAP1 cells as signal was lost at the expected MW in Early Endosome Marker knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Early Endosome Marker knockout samples were subjected to SDS-PAGE. ab109110 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110)
Predicted band size: 162 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110), expandable thumbnail](https://content.abcam.com/products/images/eea1-antibody-epr4245-early-endosome-marker-ab109110--immunocytochemistry-immunofluorescence-img69909.jpg "Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110)")
Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110)
Immunocytochemistry/Immunofluorescence analysis of JAR (human placenta choriocarcinoma epithelial) cells labelling EEA1 with ab109110 at a dilution of 1/250. Cells were fixed with 4% paraformaldehye and permeabilized with 0.1% TritonX-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody at a dilution of 1/1000. Counterstained with DAPI and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594), at a dilution of 1/200.
Image shows cytoplasmic staining in JAR cell line.
![Western blot - Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110), expandable thumbnail](https://content.abcam.com/products/images/eea1-antibody-epr4245-early-endosome-marker-ab109110--western-blot-img112166.jpg "Western blot - Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110)")
Western blot - Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110)
All lanes: Western blot - Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110) at 1/10000 dilution
Lane 1: COS-1 cell lysate at 10 µg
Lane 2: NIH 3T3 cell lysate at 10 µg
Lane 3: C6 cell lysate at 10 µg
Lane 4: HeLa cell lysate at 10 µg
Lane 5: Jurkat cell lysate at 10 µg
Lane 6: JAR cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 162 kDa
Observed band size: 170 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com