Mouse Monoclonal EED antibody. Suitable for ICC, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 7 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human EED.
View Alternative Names
Polycomb protein EED, hEED, Embryonic ectoderm development protein, WD protein associating with integrin cytoplasmic tails 1, WAIT-1, EED
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-EED antibody [163C] (AB126542)
Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling EED with ab126542 antibody at 1/40 dilution (green). DAPI was used to counterstain the nuclei (blue).
- ICC
Supplier Data
Immunocytochemistry - Anti-EED antibody [163C] (AB126542)
Immunohistochemical analysis of on frozen cytospins preparations of V5-tagged Human EED expressed in Hek293T labeling EED with ab126542 antibody (left). Cytospin preparation of V5-tagged Human MNDA protein was used as a negative control (middle). Labeling with an anti-V5 mAb confirmed the efficiency of transfection (right).
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EED antibody [163C] (AB126542)
Immunohistochemical analysis of paraffin embedded Human lymph node (top left), thymus (top right), testicle (bottom left) and bone marrow (bottom right) tissues labeling EED with ab126542 antibody at 1/600 dilution.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EED antibody [163C] (AB126542)
Immunohistochemical analysis of paraffin embedded mouse lymph node tissue labeling EED with ab126542 antibody at 1/75 dilution.
Reactivity data
Properties and storage information
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
EED functions as a repressor of gene transcription through the polycomb group complex. It interacts with other components of PRC2 such as EZH2 SUZ12 to establish and maintain chromatin modifications. EED acts as a scaffold allowing communication between other proteins essential in the assembly and function of PRC2. These interactions regulate histone methylation contributing to gene silencing.
Pathways
EED plays a central role in the regulation of the Wnt and Hedgehog pathways. These pathways are critical for controlling cell fate decisions and developmental processes. In the Wnt pathway EED in conjunction with other PRC2 members modulates β-catenin-mediated transcription. In the Hedgehog pathway EED impacts the Gli family of transcription factors underlining its importance in development and cellular proliferation regulation.
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Target data
Publications (7)
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Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2403591 PubMed39498824
2024
Applications
Unspecified application
Species
Unspecified reactive species
Cell proliferation 58:e13756 PubMed39358887
2024
Applications
Unspecified application
Species
Unspecified reactive species
iScience 25:104827 PubMed35992062
2022
Applications
Unspecified application
Species
Unspecified reactive species
Pathobiology : journal of immunopathology, molecular and cellular biology 86:152-161 PubMed31096221
2019
Applications
Unspecified application
Species
Unspecified reactive species
BMC cancer 18:158 PubMed29415665
2018
Applications
IHC-P
Species
Human
Oncotarget 5:10342-55 PubMed25431950
2014
Applications
Unspecified application
Species
Unspecified reactive species
Nature neuroscience 16:1745-53 PubMed24162653
2013
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
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