Anti-eEF1A1/EF-Tu antibody [RP23040225]

• WB

Supplier Data

Western blot - Anti-eEF1A1/EF-Tu antibody [RP23040225] (AB308036)

Western blot analysis was performed on whole cell (30 µg lysate) extracts of PC-3 (Lane 1), THP-1 (Lane 2), K-562 (Lane 3), HUVEC (Lane 4), MCF-7 (Lane 5) and tissue extract of mouse muscle (Lane 6). The blots were probed with ab308036, 1-2 µg/mL. A 50 kDa band corresponding to eEF1A1/EF-Tu was observed across the cell lines and tissue tested. The blots were detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (0.4 µg/mL, 1 : 5000 dilution). Known quantity of protein samples were electrophoresed using a 4-12% Bis-Tris gel, electrophoresis system and pre-stained protein standard. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed (ECL).

All lanes:

Western blot - Anti-eEF1A1/EF-Tu antibody [RP23040225] (ab308036) at 1 µg/mL

Lane 1:

PC-3 at 30 µg

Lane 2:

THP-1 at 30 µg

Lane 3:

K-562 at 30 µg

Lane 4:

HUVEC at 30 µg

Lane 5:

MCF-7 at 30 µg

Lane 6:

Mouse muscle lysate at 30 µg

Secondary

All lanes:

HRP-conjugated Goat anti-Rabbit IgG (H+L) Secondary Antibody at 0.4 µg/mL

Predicted band size: 50 kDa

Observed band size: 50 kDa

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