AB140632

Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9470]

Name: Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9470] (ab140632) | Abcam
Brand: Abcam Limited
SKU: AB140632
Price: 5560 USD
Availability: InStock
Rating: 5 (2 reviews)

RabMAb
Recombinant
What is this?

(2 Reviews)

(5 Publications)

Rabbit Recombinant Monoclonal eEF1A1/EF-Tu antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

View Alternative Names

EEF1A, EF1A, LENG7, EEF1A1, Elongation factor 1-alpha 1, EF-1-alpha-1, Elongation factor Tu, Eukaryotic elongation factor 1 A-1, Leukocyte receptor cluster member 7, EF-Tu, eEF1A-1, EEF1AL3, EEF1A1P5, Putative elongation factor 1-alpha-like 3, EF-1-alpha-like 3, Eukaryotic elongation factor 1 A-like 3, Eukaryotic translation elongation factor 1 alpha-1 pseudogene 5, eEF1A-like 3

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9470] (AB140632)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9470] (AB140632)

Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 with ab140632 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9470] (AB140632)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9470] (AB140632)

Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labelling eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 with purified ab140632 at a dilution of 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077 an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei counterstained with DAPI (blue).

Control : PBS only.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9470] (AB140632)

Immunohistochemical analysis of paraffin embedded Human brain tissue labelling eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5with ab140632 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9470] (AB140632)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9470] (AB140632)

Overlay histogram showing MCF7 cells stained with ab140632 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab140632 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor^® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Unknown

Western blot - Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9470] (AB140632)

All lanes:

Western blot - Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9470] (ab140632) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

MCF-7 cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-Rabbit at 1/2000 dilution

Predicted band size: 50 kDa

false

Unknown

Western blot - Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9470] (AB140632)

EE1AL3/EEF1A1P5 it's a suspected pseudogene (https : //www.ncbi.nlm.nih.gov/gene/158078). Our validation shows that this clone is able to detect Recombinant EF1AL3/EEF1A1P5 but endogenous protein expression is yet unknown.

All lanes:

Western blot - Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9470] (ab140632) at 1/1000 dilution

Lane 1:

GST tagged Recombinant Human EEF1A1 protein (Full length, 76 KDa)

Lane 2:

His tagged Recombinant Human EEF1AL3 protein (Full length, 52 KDa)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 50 kDa

false

Carrier free

Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9470] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9470]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9470

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

EE1AL3/EEF1A1P5 it's a suspected pseudogene (https://www.ncbi.nlm.nih.gov/gene/158078). Our validation shows that this clone is able to detect Recombinant EF1AL3/EEF1A1P5 but endogenous protein expression is yet unknown.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/1000", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The eEF1A1 also known as EF-Tu or eEF1A1-L3 is an elongation factor involved in protein synthesis. It facilitates the delivery of aminoacyl-tRNA to the A-site of the ribosome during translation. eEF1A1 exhibits a mass of approximately 50 kDa and is widely expressed in various tissues including the brain heart and skeletal muscle. Its expression level varies indicating that it plays a consistent role in translation machinery but may have tissue-specific functions.

Biological function summary

This elongation factor is important in promoting the accuracy and efficiency of protein translation. It interacts with several components of the protein synthesis machinery and forms part of a larger translational complex. eEF1A1 is involved in ensuring the correct amino acids are added during protein synthesis which affects protein structure and function. In addition to its canonical role it also engages in processes such as actin filament binding nuclear transport and other cell maintenance tasks.

Pathways

The activities of eEF1A1 intersect with key processes involved in cellular growth and proliferation. It plays a significant role in the mTOR signaling pathway which regulates cell growth by controlling protein synthesis. eEF1A1 also interacts with proteins like Rheb an important player in mTOR signaling. By participating in these pathways eEF1A1 links translation with broader regulatory networks that control cell metabolism.

EEF1A1 has connections to cancer and neurodegenerative diseases. Its elevated expression is often observed in several cancer types suggesting a role in tumorigenesis. Additionally misregulation of eEF1A1 may contribute to neurodegenerative disorders such as Alzheimer's disease where alterations in protein synthesis pathways are common. The protein's interaction with tau known in neuropathological conditions highlights its impact on neurodegeneration.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Translation elongation factor that catalyzes the GTP-dependent binding of aminoacyl-tRNA (aa-tRNA) to the A-site of ribosomes during the elongation phase of protein synthesis (PubMed : 26593721, PubMed : 26651998, PubMed : 36123449, PubMed : 36264623, PubMed : 36638793). Base pairing between the mRNA codon and the aa-tRNA anticodon promotes GTP hydrolysis, releasing the aa-tRNA from EEF1A1 and allowing its accommodation into the ribosome (PubMed : 26593721, PubMed : 26651998, PubMed : 36123449, PubMed : 36264623, PubMed : 36638793). The growing protein chain is subsequently transferred from the P-site peptidyl tRNA to the A-site aa-tRNA, extending it by one amino acid through ribosome-catalyzed peptide bond formation (PubMed : 26593721, PubMed : 26651998, PubMed : 36123449, PubMed : 36264623). Also plays a role in the positive regulation of IFNG transcription in T-helper 1 cells as part of an IFNG promoter-binding complex with TXK and PARP1 (PubMed : 17177976). Also plays a role in cytoskeleton organization by promoting actin bundling (By similarity).. (Microbial infection) Required for the translation of viral proteins and viral replication during human coronavirus SARS-CoV-2 infection.

See full target information EEF1A1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunology (Baltimore, Md. : 1950) 209:1189-1199 PubMed36002234

2022

A Rapid Translational Immune Response Program in CD8 Memory T Lymphocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Darin Salloum,Kamini Singh,Natalie R Davidson,Linlin Cao,David Kuo,Viraj R Sanghvi,Man Jiang,Maria Tello Lafoz,Agnes Viale,Gunnar Ratsch,Hans-Guido Wendel

Stem cell research & therapy 11:403 PubMed32943107

2020

FOXQ1 promotes the osteogenic differentiation of bone mesenchymal stem cells via Wnt/β-catenin signaling by binding with ANXA2.

Applications

Unspecified application

Species

Unspecified reactive species

Lusai Xiang,Junming Zheng,Mengdan Zhang,Tingting Ai,Bin Cai

Nature communications 9:2031 PubMed29795195

2018

Combining laser capture microdissection and proteomics reveals an active translation machinery controlling invadosome formation.

Applications

Unspecified application

Species

Unspecified reactive species

Zakaria Ezzoukhry,Elodie Henriet,Fabrice P Cordelières,Jean-William Dupuy,Marlène Maître,Nathan Gay,Sylvaine Di-Tommaso,Luc Mercier,Jacky G Goetz,Marion Peter,Frédéric Bard,Violaine Moreau,Anne-Aurélie Raymond,Frédéric Saltel

Cell 167:1229-1240.e15 PubMed27863242

2016

Decoding Mammalian Ribosome-mRNA States by Translational GTPase Complexes.

Applications

Unspecified application

Species

Unspecified reactive species

Sichen Shao,Jason Murray,Alan Brown,Jack Taunton,V Ramakrishnan,Ramanujan S Hegde

Veterinary microbiology 172:443-8 PubMed24974120

2014

EF1A interacting with nucleocapsid protein of transmissible gastroenteritis coronavirus and plays a role in virus replication.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Zhang,Hongyan Shi,Jianfei Chen,Da Shi,Changlong Li,Li Feng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9470]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9470] (AB140632)

Immunocytochemistry/ Immunofluorescence - Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9470] (AB140632)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9470] (AB140632)

Flow Cytometry (Intracellular) - Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9470] (AB140632)

Western blot - Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9470] (AB140632)

Western blot - Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9470] (AB140632)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise