Rabbit Polyclonal eEF1A1/EF-Tu antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 11 publications.
Images
Publications
- iScience 26:1073862023The cytoplasmic fraction of the histone lysine methyltransferase Setdb1 is essential for embryonic stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRoberta Rapone,Laurence Del Maestro,Costas Bouyioukos,Sonia Albini,Paola Cruz-Tapias,Véronique Joliot,Bertrand Cosson,Slimane Ait-Si-AliPubMed 37559904
- Autophagy 17:612-6252020Long-lived mice with reduced growth hormone signaling have a constitutive upregulation of hepatic chaperone-mediated autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesS Joseph Endicott,Dennis N Boynton,Logan J Beckmann,Richard A MillerPubMed 32013718
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Standard buffer
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Tested | Expected |
| Cat | Predicted | Predicted |
| Chicken | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Hamster | Predicted | Predicted |
| Horse | Predicted | Predicted |
| Macaque monkey | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Sheep | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Rabbit, Horse, Chicken, Hamster, Cow, Cat, Dog, Pig, Chimpanzee, Macaque monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Rabbit, Horse, Chicken, Hamster, Cow, Cat, Dog, Pig, Chimpanzee, Macaque monkey | Dilution info - | Notes - |
Target data
Function
This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Plays a role in the positive regulation of IFNG transcription in T-helper 1 cells as part of an IFNG promoter-binding complex with TXK and PARP1 (PubMed:17177976).(Microbial infection) Required for the translation of viral proteins and viral replication during human coronavirus SARS-CoV-2 infection.
Alternative names
Elongation factor 1-alpha 1, EF-1-alpha-1, Elongation factor Tu, Eukaryotic elongation factor 1 A-1, Leukocyte receptor cluster member 7, EF-Tu, eEF1A-1, EF1A, LENG7, EEF1A, EEF1A1
Recommended products
Rabbit Polyclonal eEF1A1/EF-Tu antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 11 publications.
Alternative names
Elongation factor 1-alpha 1, EF-1-alpha-1, Elongation factor Tu, Eukaryotic elongation factor 1 A-1, Leukocyte receptor cluster member 7, EF-Tu, eEF1A-1, EF1A, LENG7, EEF1A, EEF1A1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Standard buffer
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Specificity
Due to sequence homology the antibody recognises all three eEF1A forms, eEF1A1, eEF1A2 and eEF1A3. Through WB ab37969 was found to detect recombinant and endogenous eF1A1 and eF1A2.
- Concentration
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- Activity summary
The target eEF1A1+eEF1A2+eEF1AL3 also known by its full name as elongation factor 1-alpha consists of three isoforms involved in protein synthesis. These isoforms are encoded by different genes and have masses around 50 kDa each. eEF1A1 is expressed widely in various tissues particularly in proliferating cells while eEF1A2 expression is more restricted to muscle heart and brain tissues and eEF1AL3 is less studied regarding its tissue distribution. Mechanically these proteins play a role in the translation elongation phase facilitating the entry of aminoacyl-tRNA into the ribosome A-site.
- Biological function summary
EEF1A proteins are essential components of the translational machinery interacting with other translation factors and ribosomal subunits. As part of the eEF1 complex they aid in ensuring the fidelity of protein translation confirming that tRNAs are correctly paired with mRNA codons. These proteins also exhibit roles beyond translation influencing processes such as cytoskeleton organization suggesting that they might impact cell shape and motility.
- Pathways
EEF1A1+eEF1A2+eEF1AL3 participate mainly in protein synthesis pathways such as the mTOR signaling pathway which regulates cell growth and metabolism. They interact with ribosomal proteins and translation initiation factors that keep protein biosynthesis efficient and responsive to cellular needs. The mTOR pathway is closely connected to other pathways like AKT signaling with proteins like TSC1 and TSC2 facilitating interactions within this network.
- Associated diseases and disorders
EEF1A proteins have links to various conditions including cancer and neurodegenerative diseases. Increased eEF1A expression has been observed in certain cancers like ovarian cancer suggesting a role in cell proliferation and survival. In neurodegeneration eEF1A2 is particularly highlighted in disorders like amyotrophic lateral sclerosis (ALS) where its dysregulation may lead to neuronal cell death. The involvement of these proteins in such diverse diseases reflects their critical roles in maintaining cellular function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Western blot - Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969)
All lanes: Western blot - Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: Western blot - Jurkat whole cell lysate (Jurkat whole cell lysate ab7899) at 10 µg
Lane 3: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 10 µg
Lane 4: Western blot - HEK-293 whole cell lysate (HEK-293 whole cell lysate ab7902) at 10 µg
Lane 5: Hep G2 whole cell lysate (ab7900) at 10 µg
Lane 6: MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Lane 7: SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg
Lane 8: U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 50 kDa
Immunocytochemistry/ Immunofluorescence - Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969)
ICC/IF image of ab37969 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab37969, 1μg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Western blot - Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969)
All lanes: Western blot - Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969) at 1 µg/mL
Lane 1: Western blot - NIH/3T3 whole cell lysate (NIH/3T3 whole cell lysate ab7179) at 10 µg
Lane 2: MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 3: Brain (Mouse) Tissue Lysate at 10 µg
Lane 4: Liver (Mouse) Tissue Lysate - normal tissue at 10 µg
Lane 5: Heart (Mouse) Tissue Lysate at 10 µg
Lane 6: Kidney (Mouse) Tissue Lysate at 10 µg
Lane 7: Western blot - Mouse pancreas tissue lysate - total protein (Mouse pancreas tissue lysate - total protein ab29363) at 10 µg
Lane 8: Testis (Mouse) Tissue Lysate at 10 µg
Lane 9: Western blot - Mouse skeletal muscle tissue lysate - total protein (Mouse skeletal muscle tissue lysate - total protein ab29711) at 10 µg
Lane 10: Spinal Cord (Mouse) Tissue Lysate at 10 µg
Lane 11: Ovary (Mouse) Tissue Lysate - normal tissue at 10 µg
Lane 12: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
Lane 13: Brain (Rat) Tissue Lysate - normal tissue at 10 µg
Lane 14: Liver (Rat) Tissue Lysate at 10 µg
Lane 15: Heart (Rat) Tissue Lysate at 10 µg
Lane 16: Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480) at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 45 kDa
Western blot - Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969)
All lanes: Western blot - Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969) at 1 µg/mL
Lane 1: Western blot - Recombinant Human eEF1A1/EF-Tu protein (Recombinant Human eEF1A1/EF-Tu protein ab81792) at 0.1 µg
Lane 2: Human EEF1A2 full length protein (ab40317) at 0.1 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 50 kDa
Exposure time: 3min
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com