AB153714

Anti-EEF1A2 antibody

Name: Anti-EEF1A2 antibody (ab153714) | Abcam
Brand: Abcam Limited
SKU: AB153714

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(2 Publications)

Rabbit Polyclonal EEF1A2 antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Zebrafish, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EEF1A2 aa 1-250.

View Alternative Names

EEF1AL, STN, EEF1A2, Elongation factor 1-alpha 2, EF-1-alpha-2, Eukaryotic elongation factor 1 A-2, Statin-S1, eEF1A-2

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF1A2 antibody (AB153714)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF1A2 antibody (AB153714)

Immunohistochemical analysis of paraffin-embedded rat brain tissue stained for EEF1A2 protein at cytoplasm in rat brain with ab153714 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF1A2 antibody (AB153714)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue stained for EEF1A2 protein at cytoplasm in mouse brain with ab153714 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min.

Supplier Data

Western blot - Anti-EEF1A2 antibody (AB153714)

Samples were separated by 10 % SDS.

All lanes:

Western blot - Anti-EEF1A2 antibody (ab153714) at 1/1000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Lane 3:

HeLa whole cell extracts at 30 µg

Lane 4:

HepG2 whole cell extracts at 30 µg

Predicted band size: 50 kDa

false

Supplier Data

Western blot - Anti-EEF1A2 antibody (AB153714)

Samples were separated by 10 % SDS.

All lanes:

Western blot - Anti-EEF1A2 antibody (ab153714) at 1/1000 dilution

Lane 1:

EEF1A2 shRNA transfected 293T whole cell extracts at 30 µg

Lane 2:

Non-tranfected 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 50 kDa

false

Unknown

Western blot - Anti-EEF1A2 antibody (AB153714)

10% SDS PAGE

All lanes:

Western blot - Anti-EEF1A2 antibody (ab153714) at 1/1000 dilution

All lanes:

Mouse brain tissue lysate at 50 µg

Predicted band size: 50 kDa

false

Supplier Data

Western blot - Anti-EEF1A2 antibody (AB153714)

Samples were separated by 10 % SDS.

All lanes:

Western blot - Anti-EEF1A2 antibody (ab153714) at 1/1000 dilution

All lanes:

mouse muscle lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 50 kDa

false

Supplier Data

Western blot - Anti-EEF1A2 antibody (AB153714)

Samples were separated by 10 % SDS.

All lanes:

Western blot - Anti-EEF1A2 antibody (ab153714) at 1/1000 dilution

All lanes:

Rat brain tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 50 kDa

false

Supplier Data

Western blot - Anti-EEF1A2 antibody (AB153714)

Samples were separated by 10 % SDS.

All lanes:

Western blot - Anti-EEF1A2 antibody (ab153714) at 1/1000 dilution

Lane 1:

Zebrafish brain tissue extracts at 30 µg

Lane 2:

Zebrafish eye tissue extracts at 30 µg

Predicted band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Zebrafish, Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human EEF1A2 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q05639

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EF1A2 also known as Eukaryotic translation elongation factor 1 alpha 2 is a protein that plays a role in the elongation phase of protein synthesis. It is a GTP-binding protein with a mass of about 50 kDa. This protein facilitates the binding of aminoacyl-tRNA to the acceptor site of the ribosome during protein translation. EF1A2 is expressed mainly in heart brain and skeletal muscle tissues indicating its importance in tissues with high energy demands.

Biological function summary

EF1A2 supports cellular proliferation and growth by ensuring the continuous efficiency of protein synthesis. It participates in several protein complexes that are involved in translational control. By regulating the actin cytoskeleton EF1A2 additionally influences cell shape and motility. This multifunctionality indicates EF1A2's relevance in various cellular activities beyond its primary role in translation elongation.

Pathways

EF1A2 integrates into the protein synthesis pathway and also plays a role in the mTOR signaling pathway which regulates cell growth and metabolism. In the mTOR pathway EF1A2 works alongside other elongation factors like EF2 and components of the translation machinery to coordinate protein synthesis. It interacts with proteins involved in nutrient signaling and energy status showing its role in integrating metabolic signals with translational processes.

EF1A2 shows a connection with cancer and neurodegenerative diseases. Overexpression of EF1A2 has been linked to several types of cancer as its role in facilitating protein synthesis can contribute to uncontrolled cellular proliferation. In neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) altered expression of EF1A2 suggests a disruption in protein homeostasis. The dysregulation of EF1A2 potentially affecting actin-binding proteins like TDP-43 highlights its association with disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

See full target information EEF1A2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Chemical biology & drug design 104:e14602 PubMed39134897

2024

Promotion Effect of Catalpol on Angiogenesis and Potential Mechanisms: A Research Based on Network Pharmacology.

Applications

Unspecified application

Species

Unspecified reactive species

Jin-Rong Ni,Qun-Hu Zhang,Jie-Lin Deng,Hai-Hu Wang,Yong-Chi Duan,Cheng-Ji Zhang,Lue-Tao Jiang

Journal of cell science 129:4455-4465 PubMed27807005

2016

Inappropriate expression of the translation elongation factor 1A disrupts genome stability and metabolism.

Applications

Species

Human

Daniel J Tarrant,Mariarita Stirpe,Michelle Rowe,Mark J Howard,Tobias von der Haar,Campbell W Gourlay

View all publications

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