AB212172

Anti-EEF1A2 antibody

Name: Anti-EEF1A2 antibody (ab212172) | Abcam
Brand: Abcam Limited
SKU: AB212172
Price: 545 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal EEF1A2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EEF1A2 aa 200-300.

View Alternative Names

EEF1AL, STN, EEF1A2, Elongation factor 1-alpha 2, EF-1-alpha-2, Eukaryotic elongation factor 1 A-2, Statin-S1, eEF1A-2

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF1A2 antibody (AB212172)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF1A2 antibody (AB212172)

Immunohistochemical analysis of human skeletal muscle labeling EEF1A2 with ab212172 at a 1/200 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-EEF1A2 antibody (AB212172)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-EEF1A2 antibody (AB212172)

Immunocytochemical analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) whole cells labeling EEF1A2 in the cytosol with ab212172 at 2 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF1A2 antibody (AB212172)

Immunohistochemical analysis of human fallopian tube labeling EEF1A2 with ab212172 at a 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF1A2 antibody (AB212172)

Immunohistochemical analysis of human duodenum labeling EEF1A2 with ab212172 at a 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF1A2 antibody (AB212172)

Immunohistochemical analysis of human kidney labeling EEF1A2 with ab212172 at a 1/200 dilution.

Supplier Data

Western blot - Anti-EEF1A2 antibody (AB212172)

All lanes:

Western blot - Anti-EEF1A2 antibody (ab212172) at 0.4 µg/mL

Lane 1:

RT4 (Human urinary bladder cancer cell line) whole cell lysate

Lane 2:

U-251 MG (formally U-373 MG) (Human brain glioma cell line) whole cell lysate

Predicted band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human EEF1A2 aa 200-300. The exact immunogen used to generate this antibody is proprietary information.

Q05639

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EF1A2 also known as Eukaryotic translation elongation factor 1 alpha 2 is a protein that plays a role in the elongation phase of protein synthesis. It is a GTP-binding protein with a mass of about 50 kDa. This protein facilitates the binding of aminoacyl-tRNA to the acceptor site of the ribosome during protein translation. EF1A2 is expressed mainly in heart brain and skeletal muscle tissues indicating its importance in tissues with high energy demands.

Biological function summary

EF1A2 supports cellular proliferation and growth by ensuring the continuous efficiency of protein synthesis. It participates in several protein complexes that are involved in translational control. By regulating the actin cytoskeleton EF1A2 additionally influences cell shape and motility. This multifunctionality indicates EF1A2's relevance in various cellular activities beyond its primary role in translation elongation.

Pathways

EF1A2 integrates into the protein synthesis pathway and also plays a role in the mTOR signaling pathway which regulates cell growth and metabolism. In the mTOR pathway EF1A2 works alongside other elongation factors like EF2 and components of the translation machinery to coordinate protein synthesis. It interacts with proteins involved in nutrient signaling and energy status showing its role in integrating metabolic signals with translational processes.

EF1A2 shows a connection with cancer and neurodegenerative diseases. Overexpression of EF1A2 has been linked to several types of cancer as its role in facilitating protein synthesis can contribute to uncontrolled cellular proliferation. In neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) altered expression of EF1A2 suggests a disruption in protein homeostasis. The dysregulation of EF1A2 potentially affecting actin-binding proteins like TDP-43 highlights its association with disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

See full target information EEF1A2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Experimental hematology & oncology 12:49 PubMed37221625

2023

ScRNA-seq revealed an immunosuppression state and tumor microenvironment heterogeneity related to lymph node metastasis in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shiyong Xin,Xiang Liu,Ziyao Li,Xianchao Sun,Rong Wang,Zhenhua Zhang,Xinwei Feng,Liang Jin,Weiyi Li,Chaozhi Tang,Wangli Mei,Qiong Cao,Haojie Wang,Jianguo Zhang,Lijin Feng,Lin Ye

Immunity, inflammation and disease 9:274-287 PubMed33332777

2020

The BTLA and PD-1 signaling pathways independently regulate the proliferation and cytotoxicity of human peripheral blood γδ T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hyun J Hwang,Jae J Lee,Sung H Kang,Jin K Suh,Eun S Choi,Seongsoo Jang,Sang-Hyun Hwang,Kyung-Nam Koh,Ho J Im,Nayoung Kim

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EEF1A2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF1A2 antibody (AB212172)

Immunocytochemistry/ Immunofluorescence - Anti-EEF1A2 antibody (AB212172)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF1A2 antibody (AB212172)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF1A2 antibody (AB212172)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF1A2 antibody (AB212172)

Western blot - Anti-EEF1A2 antibody (AB212172)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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