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Rabbit Recombinant Monoclonal EEF1A2 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

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Images

Western blot - Anti-EEF1A2 antibody [EPR22651-46] (AB227824), expandable thumbnail
  • Western blot - Anti-EEF1A2 antibody [EPR22651-46] (AB227824), expandable thumbnail
  • Immunoprecipitation - Anti-EEF1A2 antibody [EPR22651-46] (AB227824), expandable thumbnail
  • Western blot - Anti-EEF1A2 antibody [EPR22651-46] (AB227824), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 49% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IF
Human
Tested
Not recommended
Tested
Not recommended
Not recommended
Mouse
Expected
Not recommended
Tested
Not recommended
Not recommended
Rat
Expected
Not recommended
Tested
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Alternative names

EEF1AL, STN, EEF1A2, Elongation factor 1-alpha 2, EF-1-alpha-2, Eukaryotic elongation factor 1 A-2, Statin-S1, eEF1A-2

Recommended products

Rabbit Recombinant Monoclonal EEF1A2 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22651-46
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

EF1A2 also known as Eukaryotic translation elongation factor 1 alpha 2 is a protein that plays a role in the elongation phase of protein synthesis. It is a GTP-binding protein with a mass of about 50 kDa. This protein facilitates the binding of aminoacyl-tRNA to the acceptor site of the ribosome during protein translation. EF1A2 is expressed mainly in heart brain and skeletal muscle tissues indicating its importance in tissues with high energy demands.

Biological function summary

EF1A2 supports cellular proliferation and growth by ensuring the continuous efficiency of protein synthesis. It participates in several protein complexes that are involved in translational control. By regulating the actin cytoskeleton EF1A2 additionally influences cell shape and motility. This multifunctionality indicates EF1A2's relevance in various cellular activities beyond its primary role in translation elongation.

Pathways

EF1A2 integrates into the protein synthesis pathway and also plays a role in the mTOR signaling pathway which regulates cell growth and metabolism. In the mTOR pathway EF1A2 works alongside other elongation factors like EF2 and components of the translation machinery to coordinate protein synthesis. It interacts with proteins involved in nutrient signaling and energy status showing its role in integrating metabolic signals with translational processes.

Associated diseases and disorders

EF1A2 shows a connection with cancer and neurodegenerative diseases. Overexpression of EF1A2 has been linked to several types of cancer as its role in facilitating protein synthesis can contribute to uncontrolled cellular proliferation. In neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) altered expression of EF1A2 suggests a disruption in protein homeostasis. The dysregulation of EF1A2 potentially affecting actin-binding proteins like TDP-43 highlights its association with disease pathology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-EEF1A2 antibody [EPR22651-46] (ab227824), expandable thumbnail

    Western blot - Anti-EEF1A2 antibody [EPR22651-46] (ab227824)

    ab227824 was shown to specifically react with EEF1A2 in wild-type HAP1 cells as signal was lost in EEF1A2 knockout cells. Wild-type and EEF1A2 knockout samples were subjected to SDS-PAGE. ab227824 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

    This antibody also recognizes a weakly unidentifiable band at around 75kDa

    Blocking/diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 3.25 seconds

    All lanes: Western blot - Anti-EEF1A2 antibody [EPR22651-46] (ab227824) at 1/1000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: EEF1A2 knockout HAP1 whole cell lysate at 20 µg

    Lane 3: HEK-293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

    Lane 4: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 50 kDa

    Observed band size: 50 kDa

  • Western blot - Anti-EEF1A2 antibody [EPR22651-46] (ab227824), expandable thumbnail

    Western blot - Anti-EEF1A2 antibody [EPR22651-46] (ab227824)

    Blocking/diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 7.75 seconds

    All lanes: Western blot - Anti-EEF1A2 antibody [EPR22651-46] (ab227824) at 1/1000 dilution

    All lanes: Human brain tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 50 kDa

    Observed band size: 50 kDa

  • Immunoprecipitation - Anti-EEF1A2 antibody [EPR22651-46] (ab227824), expandable thumbnail

    Immunoprecipitation - Anti-EEF1A2 antibody [EPR22651-46] (ab227824)

    EEF1A2 was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney epithelial cell) whole cell lysate with ab227824 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab227824 1/1000 dilution (0.54 μg/ml). VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used as the secondary antibody at 1/5000 dilution.

    Lane 1: HEK-293T (human embryonic kidney epithelial cell) whole cell lysate 10μg

    Lane 2: ab227824 IP in HEK-293T whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab227824 in HEK-293T whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds

    All lanes: Immunoprecipitation - Anti-EEF1A2 antibody [EPR22651-46] (ab227824)

    Predicted band size: 47 kDa, 50 kDa

    Observed band size: 46 kDa, 50 kDa

  • Western blot - Anti-EEF1A2 antibody [EPR22651-46] (ab227824), expandable thumbnail

    Western blot - Anti-EEF1A2 antibody [EPR22651-46] (ab227824)

    Blocking/diluting buffer and concentration: 5% NFDM/TBST

    Exposure Time: 3.25 seconds

    All lanes: Western blot - Anti-EEF1A2 antibody [EPR22651-46] (ab227824) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Mouse heart tissue lysate at 20 µg

    Lane 3: Rat brain tissue lysate at 20 µg

    Lane 4: Rat heart tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 50 kDa

    Observed band size: 50 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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