JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB153728

Anti-EEF1D antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal EEF1D antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EEF1D aa 1 to C-terminus.

View Alternative Names

EF1D, EEF1D, Elongation factor 1-delta, EF-1-delta, Antigen NY-CO-4

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-EEF1D antibody (AB153728)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EEF1D antibody (AB153728)

Immunofluorescence analysis of methanol-fixed A431 cells, labeling EEF1D using ab153728 at 1/200 dilution. Right panel costained with Hoechst 33342.

Western blot - Anti-EEF1D antibody (AB153728)
  • WB

Unknown

Western blot - Anti-EEF1D antibody (AB153728)

12% SDS PAGE

All lanes:

Western blot - Anti-EEF1D antibody (ab153728) at 1/1000 dilution

All lanes:

H1299 cell lysate at 30 µg

Predicted band size: 31 kDa

false

Western blot - Anti-EEF1D antibody (AB153728)
  • WB

Unknown

Western blot - Anti-EEF1D antibody (AB153728)

10% SDS PAGE

All lanes:

Western blot - Anti-EEF1D antibody (ab153728) at 1/3000 dilution

All lanes:

Mouse brain tissue lysate at 50 µg

Predicted band size: 31 kDa,76 kDa,86 kDa

Observed band size: 86 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human EEF1D aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P29692

style
left-column

Alternative Products

Primary Antibodies

AB228803

Anti-EEF1D antibody

Immunocytochemistry/ Immunofluorescence - Anti-EEF1D antibody (AB228803)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB248088

Anti-Elongation factor 1-gamma antibody [EPR7200] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Elongation factor 1-gamma antibody [EPR7200] - BSA and Azide free (AB248088)

  • 0
  • 0
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Primary Antibodies

AB140632

Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9470]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9470] (AB140632)

  • 5
  • 2
Primary Antibodies

AB15828

Anti-V5 tag antibody

Lab Essentials

Western blot - Anti-V5 tag antibody (AB15828)

  • 4
  • 1
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EEF1D also known as elongation factor 1-delta plays an important role in protein synthesis by facilitating the delivery of aminoacyl-tRNA to the ribosome A-site during translation elongation. The protein has a molecular mass of approximately 33 kDa. EEF1D is expressed in various tissues with notable presence in the heart and skeletal muscle. This expression pattern suggests its involvement in tissue-specific synthesis of proteins needed for diverse cellular functions.
Biological function summary

EEF1D interacts with other components of the translational machinery to form the elongation factor complex which is essential for efficient protein biosynthesis. EEF1D's interaction with the EF1B complex supports the recycling of guanine nucleotides which prepares EF1A for reuse in additional rounds of protein elongation. This collaboration within the machinery highlights EEF1D's importance in maintaining proper protein synthesis rates which are important for cellular growth and repair processes.

Pathways

EEF1D is integral in the translation and protein metabolism pathways. The protein's roles align closely with those of EEF1A and EEF1B ensuring smooth continuation of the elongation phase of protein synthesis. Its participation in these pathways highlights how alterations in EEF1D function might disrupt broader cellular processes impacting how cells respond to genetic instructions and manage protein construction workflows.

EEF1D has connections to cancer and neurodegenerative diseases. Abnormal expression or mutations in EEF1D can influence tumor progression by altering protein synthesis rates or pathways related to cell proliferation. Additionally connections exist between EEF1D and EEF1A2 in neurodegenerative conditions where disruptions in protein synthesis contribute to neuronal damage and disease manifestation. Understanding EEF1D's role in these conditions may offer insight into potential therapeutic targets for intervention.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Isoform 1. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1-alpha for another round of transfer of aminoacyl-tRNAs to the ribosome.. Isoform 2. Regulates induction of heat-shock-responsive genes through association with heat shock transcription factors and direct DNA-binding at heat shock promoter elements (HSE).
See full target information EEF1D
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1289175 PubMed38332912

2024

Autoantibodies against eukaryotic translation elongation factor 1 delta in two patients with autoimmune cerebellar ataxia.

Applications

Unspecified application

Species

Unspecified reactive species

Liyuan Guo,Haitao Ren,Siyuan Fan,Xingchen Chao,Mange Liu,Hongzhi Guan,Jing Wang

Cell reports 26:1286-1302.e8 PubMed30699355

2019

Integrated Phosphoproteome and Transcriptome Analysis Reveals Chlamydia-Induced Epithelial-to-Mesenchymal Transition in Host Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Piotr K Zadora,Cindrilla Chumduri,Koshi Imami,Hilmar Berger,Yang Mi,Matthias Selbach,Thomas F Meyer,Rajendra Kumar Gurumurthy
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com