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AB228803

Anti-EEF1D antibody

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(1 Publication)

Rabbit Polyclonal EEF1D antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human EEF1D.

View Alternative Names

EF1D, EEF1D, Elongation factor 1-delta, EF-1-delta, Antigen NY-CO-4

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-EEF1D antibody (AB228803)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-EEF1D antibody (AB228803)

Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for EEF1D (red) using ab228803 at 1/500 dilution in ICC/IF.

Blue : Hoechst 33342 staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF1D antibody (AB228803)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF1D antibody (AB228803)

Paraffin-embedded human adenocarcinoma tissue stained for EEF1D using ab228803 at 1/250 dilution in immunohistochemical analysis.

Western blot - Anti-EEF1D antibody (AB228803)
  • WB

Supplier Data

Western blot - Anti-EEF1D antibody (AB228803)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-EEF1D antibody (ab228803) at 1/1000 dilution

All lanes:

A549 (human lung carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 31 kDa

false

Western blot - Anti-EEF1D antibody (AB228803)
  • WB

Supplier Data

Western blot - Anti-EEF1D antibody (AB228803)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-EEF1D antibody (ab228803) at 1/1000 dilution

Lane 1:

THP-1 (human monocytic leukemia cell line) whole cell lysate at 30 µg

Lane 2:

HL-60 (human promyelocytic leukemia cell line) whole cell lysate at 30 µg

Lane 3:

NCI-H929 whole cell lysate at 30 µg

Predicted band size: 101 kDa,31 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human EEF1D. The exact immunogen used to generate this antibody is proprietary information.

P29692

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EEF1D also known as elongation factor 1-delta plays an important role in protein synthesis by facilitating the delivery of aminoacyl-tRNA to the ribosome A-site during translation elongation. The protein has a molecular mass of approximately 33 kDa. EEF1D is expressed in various tissues with notable presence in the heart and skeletal muscle. This expression pattern suggests its involvement in tissue-specific synthesis of proteins needed for diverse cellular functions.
Biological function summary

EEF1D interacts with other components of the translational machinery to form the elongation factor complex which is essential for efficient protein biosynthesis. EEF1D's interaction with the EF1B complex supports the recycling of guanine nucleotides which prepares EF1A for reuse in additional rounds of protein elongation. This collaboration within the machinery highlights EEF1D's importance in maintaining proper protein synthesis rates which are important for cellular growth and repair processes.

Pathways

EEF1D is integral in the translation and protein metabolism pathways. The protein's roles align closely with those of EEF1A and EEF1B ensuring smooth continuation of the elongation phase of protein synthesis. Its participation in these pathways highlights how alterations in EEF1D function might disrupt broader cellular processes impacting how cells respond to genetic instructions and manage protein construction workflows.

EEF1D has connections to cancer and neurodegenerative diseases. Abnormal expression or mutations in EEF1D can influence tumor progression by altering protein synthesis rates or pathways related to cell proliferation. Additionally connections exist between EEF1D and EEF1A2 in neurodegenerative conditions where disruptions in protein synthesis contribute to neuronal damage and disease manifestation. Understanding EEF1D's role in these conditions may offer insight into potential therapeutic targets for intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Isoform 1. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1-alpha for another round of transfer of aminoacyl-tRNAs to the ribosome.. Isoform 2. Regulates induction of heat-shock-responsive genes through association with heat shock transcription factors and direct DNA-binding at heat shock promoter elements (HSE).
See full target information EEF1D
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Phytotherapy research : PTR 35:2200-2210 PubMed33258205

2020

Chlorogenic acid depresses cellular bioenergetics to suppress pancreatic carcinoma through modulating c-Myc-TFR1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Hongbao Yang,Abdullahi Mohamed Said,Huimin Huang,Akuetteh Percy David Papa,Guihua Jin,Shijia Wu,Nengfang Ma,Linhua Lan,Fugen Shangguan,Qiyu Zhang
View all publications
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Product promise

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