Rabbit Polyclonal EEF1D antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human EEF1D.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human EEF1D. The exact immunogen used to generate this antibody is proprietary information. Database link P29692
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Isoform 1. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1-alpha for another round of transfer of aminoacyl-tRNAs to the ribosome. Isoform 2. Regulates induction of heat-shock-responsive genes through association with heat shock transcription factors and direct DNA-binding at heat shock promoter elements (HSE).
Alternative names
EF1D, EEF1D, Elongation factor 1-delta, EF-1-delta, Antigen NY-CO-4
Recommended products
Rabbit Polyclonal EEF1D antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human EEF1D.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human EEF1D. The exact immunogen used to generate this antibody is proprietary information. Database link P29692
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EEF1D also known as elongation factor 1-delta plays an important role in protein synthesis by facilitating the delivery of aminoacyl-tRNA to the ribosome A-site during translation elongation. The protein has a molecular mass of approximately 33 kDa. EEF1D is expressed in various tissues with notable presence in the heart and skeletal muscle. This expression pattern suggests its involvement in tissue-specific synthesis of proteins needed for diverse cellular functions.
- Biological function summary
EEF1D interacts with other components of the translational machinery to form the elongation factor complex which is essential for efficient protein biosynthesis. EEF1D's interaction with the EF1B complex supports the recycling of guanine nucleotides which prepares EF1A for reuse in additional rounds of protein elongation. This collaboration within the machinery highlights EEF1D's importance in maintaining proper protein synthesis rates which are important for cellular growth and repair processes.
- Pathways
EEF1D is integral in the translation and protein metabolism pathways. The protein's roles align closely with those of EEF1A and EEF1B ensuring smooth continuation of the elongation phase of protein synthesis. Its participation in these pathways highlights how alterations in EEF1D function might disrupt broader cellular processes impacting how cells respond to genetic instructions and manage protein construction workflows.
- Associated diseases and disorders
EEF1D has connections to cancer and neurodegenerative diseases. Abnormal expression or mutations in EEF1D can influence tumor progression by altering protein synthesis rates or pathways related to cell proliferation. Additionally connections exist between EEF1D and EEF1A2 in neurodegenerative conditions where disruptions in protein synthesis contribute to neuronal damage and disease manifestation. Understanding EEF1D's role in these conditions may offer insight into potential therapeutic targets for intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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4 product images
Western blot - Anti-EEF1D antibody (ab228803)
10% SDS-PAGE gel.
All lanes: Western blot - Anti-EEF1D antibody (ab228803) at 1/1000 dilution
All lanes: A549 (human lung carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 31 kDa
Immunocytochemistry/ Immunofluorescence - Anti-EEF1D antibody (ab228803)
Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for EEF1D (red) using ab228803 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF1D antibody (ab228803)
Paraffin-embedded human adenocarcinoma tissue stained for EEF1D using ab228803 at 1/250 dilution in immunohistochemical analysis.
Western blot - Anti-EEF1D antibody (ab228803)
10% SDS-PAGE gel.
All lanes: Western blot - Anti-EEF1D antibody (ab228803) at 1/1000 dilution
Lane 1: THP-1 (human monocytic leukemia cell line) whole cell lysate at 30 µg
Lane 2: HL-60 (human promyelocytic leukemia cell line) whole cell lysate at 30 µg
Lane 3: NCI-H929 whole cell lysate at 30 µg
Predicted band size: 101 kDa, 31 kDa
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