AB131202

Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5]

Name: Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202) | Abcam
Brand: Abcam Limited
SKU: AB131202
Price: 500 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(3 Publications)

Mouse Monoclonal EEF2/Elongation factor 2 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat, African green monkey samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EEF2.

View Alternative Names

EF2, EEF2, Elongation factor 2, EF-2

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (AB131202)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (AB131202)

Immunocytochemical staining of HeLa cells using anti-eEF2/Elongation factor 2 antibody (ab131202) at a dilution of 1 : 200. Cells were fixed with ice cold methanol. This image was produced with antibody produced in ascites.

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Western blot - Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (AB131202)

This image was produced with antibody produced in ascites.

All lanes:

Western blot - Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202) at 1/1000 dilution

All lanes:

HL-60 cell lysate

Predicted band size: 95 kDa

Observed band size: 95 kDa

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Supplier Data

Western blot - Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (AB131202)

All lanes:

Western blot - Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202) at 1/5000 dilution

Lane 1:

Jurkat Cell lysates

Lane 2:

C6 Cell lysates

Lane 3:

3T3 Cell lysates

Lane 4:

COS-7 (African green monkey kidney fibroblast-like cell line) Cell lysates

Lane 5:

HeLa Cell lysates

Predicted band size: 95 kDa

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Supplier Data

Western blot - Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (AB131202)

Western blot detection of EEF2/Elongation factor 2 in HL-60,Jurkat,SHSY-5Y,U20S and Hela cell lysates using EEF2/Elongation factor 2 mouse mAb (1 : 5000).Predicted band size : 95KDa.Observed band size : 95KDa.Exposure time : 15s. This image was produced with antibody produced in ascites.

All lanes:

Western blot - Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202)

Predicted band size: 95 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4B3-G7-H5

Isotype

IgG2b

Carrier free

Reacts with

Mouse, Rat, Human, African green monkey

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human EEF2. The exact immunogen used to generate this antibody is proprietary information.

P13639

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

This product was changed from ascites to tissue culture supernatant on 17.08.2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Purification technique

Cross-flow filtration

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EEF2 or elongation factor 2 is an important protein in the process of translation during protein synthesis. It is also known by the names EF-2 and EF2. This protein facilitates the translocation step where it moves tRNA and mRNA from the A-site to the P-site on the ribosome. EEF2 has a molecular weight of approximately 95 kDa. The protein is ubiquitously expressed in various tissues and cells due to its fundamental role in protein synthesis making it essential for cellular activities.

Biological function summary

EEF2 functions as part of a larger ribosomal complex. It has an important role in ensuring the accuracy and efficiency of elongation during the translation process. This process is energy-dependent with EEF2 requiring GTP to catalyze the movement along the mRNA strand. The protein must undergo reversible phosphorylation which directly affects its activity. This post-translational modification of EEF2 is a significant modulation point influencing the rate of protein synthesis.

Pathways

EEF2 plays an integral role in the mTOR signaling pathway and the insulin signaling pathway. In the mTOR pathway EEF2 activity is regulated to control protein translation based on cellular energy status and nutrient availability. This pathway is important for cell growth and proliferation. EEF2 interacts with other proteins such as factor 2 and eEF2kinase which phosphorylates the protein thereby inhibiting its activity. In the insulin signaling pathway similar regulatory mechanisms impact EEF2’s role in translation.

EEF2 connects to cancer and neurodegenerative diseases. In cancer aberrant regulation of EEF2 can lead to uncontrolled protein synthesis promoting cell proliferation and tumor growth. Its interaction with mTOR and other signaling proteins like ribosomal proteins plays a role in this dysregulation. In neurodegenerative conditions such as Alzheimer’s disease impaired EEF2 activity results in decreased synaptic protein synthesis affecting neuronal function. Understanding EEF2’s regulation and dysregulation provides insight into its involvement in these diseases offering potential for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation (PubMed : 26593721). During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively (PubMed : 26593721). Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (PubMed : 26593721).

See full target information EEF2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Science advances 10:eadf7001 PubMed38608030

2024

The autism susceptibility kinase, TAOK2, phosphorylates eEF2 and modulates translation.

Applications

Unspecified application

Species

Unspecified reactive species

Melad Henis,Tabitha Rücker,Robin Scharrenberg,Melanie Richter,Lucas Baltussen,Shuai Hong,Durga Praveen Meka,Birgit Schwanke,Nagammal Neelagandan,Danie Daaboul,Nadeem Murtaza,Christoph Krisp,Sönke Harder,Hartmut Schlüter,Matthias Kneussel,Irm Hermans-Borgmeyer,Joris de Wit,Karun K Singh,Kent E Duncan,Froylan Calderón de Anda

Molecular cell 81:3065-3081.e12 PubMed34297911

2021

Transcription-mediated supercoiling regulates genome folding and loop formation.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Victoria Neguembor,Laura Martin,Álvaro Castells-García,Pablo Aurelio Gómez-García,Chiara Vicario,Davide Carnevali,Jumana AlHaj Abed,Alba Granados,Ruben Sebastian-Perez,Francesco Sottile,Jérôme Solon,Chao-Ting Wu,Melike Lakadamyali,Maria Pia Cosma

Molecular cell 81:1425-1438.e10 PubMed33662272

2021

PQBP1 promotes translational elongation and regulates hippocampal mGluR-LTD by suppressing eEF2 phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuqian Shen,Zi Chao Zhang,Shanshan Cheng,An Liu,Jian Zuo,Shuting Xia,Xian Liu,Wenhua Liu,Zhengping Jia,Wei Xie,Junhai Han

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5]

Immunocytochemistry/ Immunofluorescence - Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (AB131202)

Western blot - Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (AB131202)

Western blot - Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (AB131202)

Western blot - Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (AB131202)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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