Rabbit Polyclonal EEF2/Elongation factor 2 antibody. Suitable for IHC-P, ICC/IF, IP, WB and reacts with Human, Mouse, Zebrafish, Rat samples. Cited in 15 publications.
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- Journal of experimental & clinical cancer research : CR 42:972023Toosendanin targeting eEF2 impedes Topoisomerase I & II protein translation to suppress esophageal squamous cell carcinoma growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuechao Jia,Penglei Wang,Chuntian Huang,Dengyun Zhao,Qiong Wu,Bingbing Lu,Wenna Nie,Limeng Huang,Xueli Tian,Pan Li,Kyle Vaughn Laster,Yanan Jiang,Xiang Li,Honglin Li,Zigang Dong,Kangdong LiuPubMed 37088855
- Frontiers in molecular neuroscience 16:10489852023Roxadustat (FG-4592) abated lipopolysaccharides-induced depressive-like symptoms PI3K signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAxiang Li,Zizhen Liu,Tahir Ali,Ruyan Gao,Yanhua Luo,Qichao Gong,Chenyou Zheng,Weifen Li,Hongling Guo,Xinshe Liu,Shupeng Li,Tao LiPubMed 37008780
- Science advances 8:eabo60492022C11orf94/Frey is a key regulator for male fertility by controlling Izumo1 complex assembly.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWhendy Contreras,Caroline Wiesehöfer,Dora Schreier,Nadja Leinung,Petra Peche,Gunther Wennemuth,Marc Gentzel,Bernd Schröder,Torben MentrupPubMed 35960805
- Nature communications 13:18802022Phagosomal signalling of the C-type lectin receptor Dectin-1 is terminated by intramembrane proteolysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTorben Mentrup,Anna Yamina Stumpff-Niggemann,Nadja Leinung,Christine Schlosser,Katja Schubert,Rebekka Wehner,Antje Tunger,Valentin Schatz,Patrick Neubert,Ann-Christine Gradtke,Janina Wolf,Stefan Rose-John,Paul Saftig,Alexander Dalpke,Jonathan Jantsch,Marc Schmitz,Regina Fluhrer,Ilse D Jacobsen,Bernd SchröderPubMed 35388002
- Molecular cell 75:631-643.e82019Identification of the mAm Methyltransferase PCIF1 Reveals the Location and Functions of mAm in the Transcriptome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKonstantinos Boulias et. al.PubMed 31279658
- Journal of dairy science 102:4264-42742019Phosphorylation of AKT serine/threonine kinase and abundance of milk protein synthesis gene networks in mammary tissue in response to supply of methionine in periparturient Holstein cows.Applications:Unspecified applicationReactive species:Unspecified reactive speciesY F Ma et. al.PubMed 30879806
- EMBO reports 20:2019The intramembrane protease SPPL2c promotes male germ cell development by cleaving phospholamban.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohannes Niemeyer et. al.PubMed 30733280
- Communications biology 1:1552018Doxorubicin induces caspase-mediated proteolysis of KV7.1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnne Strigli et. al.PubMed 30302399
- Autophagy 13:670-6852017Disruption of the vacuolar-type H-ATPase complex in liver causes MTORC1-independent accumulation of autophagic vacuoles and lysosomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSandra Kissing et. al.PubMed 28129027
- The journal of physiological sciences : JPS 68:43-532016Branched-chain amino acids administration suppresses endurance exercise-related activation of ubiquitin proteasome signaling in trained human skeletal muscle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEvgeny A Lysenko,Tatiana F Vepkhvadze,Egor M Lednev,Olga L Vinogradova,Daniil V PopovPubMed 27913948
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| IHC-P | ICC/IF | IP | WB | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Expected | Expected | Tested |
| Rat | Expected | Expected | Expected | Tested |
| Chicken | Predicted | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted | Predicted |
| Hamster | Predicted | Predicted | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted | Predicted | Predicted |
| Zebrafish | Expected | Expected | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Species Zebrafish | Dilution info - | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Hamster, Cow, Xenopus laevis | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Species Zebrafish | Dilution info - | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Hamster, Cow, Xenopus laevis | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Species Zebrafish | Dilution info - | Notes - |
Species Rat | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Hamster, Cow, Xenopus laevis | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Zebrafish, Human, Rat | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Hamster, Cow, Xenopus laevis | Dilution info - | Notes - |
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Target data
Function
Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
Alternative names
Elongation factor 2, EF-2, EEF2, EF2
Recommended products
Rabbit Polyclonal EEF2/Elongation factor 2 antibody. Suitable for IHC-P, ICC/IF, IP, WB and reacts with Human, Mouse, Zebrafish, Rat samples. Cited in 15 publications.
Alternative names
Elongation factor 2, EF-2, EEF2, EF2
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EEF2 or elongation factor 2 is an important protein in the process of translation during protein synthesis. It is also known by the names EF-2 and EF2. This protein facilitates the translocation step where it moves tRNA and mRNA from the A-site to the P-site on the ribosome. EEF2 has a molecular weight of approximately 95 kDa. The protein is ubiquitously expressed in various tissues and cells due to its fundamental role in protein synthesis making it essential for cellular activities.
- Biological function summary
EEF2 functions as part of a larger ribosomal complex. It has an important role in ensuring the accuracy and efficiency of elongation during the translation process. This process is energy-dependent with EEF2 requiring GTP to catalyze the movement along the mRNA strand. The protein must undergo reversible phosphorylation which directly affects its activity. This post-translational modification of EEF2 is a significant modulation point influencing the rate of protein synthesis.
- Pathways
EEF2 plays an integral role in the mTOR signaling pathway and the insulin signaling pathway. In the mTOR pathway EEF2 activity is regulated to control protein translation based on cellular energy status and nutrient availability. This pathway is important for cell growth and proliferation. EEF2 interacts with other proteins such as factor 2 and eEF2kinase which phosphorylates the protein thereby inhibiting its activity. In the insulin signaling pathway similar regulatory mechanisms impact EEF2’s role in translation.
- Associated diseases and disorders
EEF2 connects to cancer and neurodegenerative diseases. In cancer aberrant regulation of EEF2 can lead to uncontrolled protein synthesis promoting cell proliferation and tumor growth. Its interaction with mTOR and other signaling proteins like ribosomal proteins plays a role in this dysregulation. In neurodegenerative conditions such as Alzheimer’s disease impaired EEF2 activity results in decreased synaptic protein synthesis affecting neuronal function. Understanding EEF2’s regulation and dysregulation provides insight into its involvement in these diseases offering potential for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Immunocytochemistry/ Immunofluorescence - Anti-EEF2/Elongation factor 2 antibody (ab33523)
ab33523 staining EEF2 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab33523 at 1μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Western blot - Anti-EEF2/Elongation factor 2 antibody (ab33523)
All lanes: Western blot - Anti-EEF2/Elongation factor 2 antibody (ab33523) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: Western blot - Jurkat whole cell lysate (Jurkat whole cell lysate ab7899) at 10 µg
Lane 3: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 10 µg
Lane 4: Western blot - HEK-293 whole cell lysate (HEK-293 whole cell lysate ab7902) at 10 µg
Lane 5: Hep G2 whole cell lysate (ab7900) at 10 µg
Lane 6: MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Lane 7: SHSY-5Y (Human Neuroblastoma) Whole Cell Lysate at 10 µg
Lane 8: U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 95 kDa
Observed band size: 95 kDa
Western blot - Anti-EEF2/Elongation factor 2 antibody (ab33523)
All lanes: Western blot - Anti-EEF2/Elongation factor 2 antibody (ab33523) at 1 µg/mL
Lane 1: Western blot - NIH/3T3 whole cell lysate (NIH/3T3 whole cell lysate ab7179) at 10 µg
Lane 2: MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 3: Western blot - Mouse pancreas tissue lysate - total protein (Mouse pancreas tissue lysate - total protein ab29363) at 10 µg
Lane 4: Ovary (Mouse) Tissue Lysate - normal tissue at 10 µg
Lane 5: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
Lane 6: Liver (Rat) Tissue Lysate - normal tissue at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 95 kDa
Observed band size: 95 kDa
Immunoprecipitation - Anti-EEF2/Elongation factor 2 antibody (ab33523)
EEF2/Elongation factor 2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5μg of Rabbit polyclonal to EEF2/Elongation factor 2 and 50μl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40μl SDS loading buffer and incubated for 10min at 70oC; 10μl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab33523.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (HRP) ab99697).
Band: 95kDa; EEF2/Elongation factor 2, non-specific band: 135kDa,due to background seen in No ab control lane (2).All lanes: Immunoprecipitation - Anti-EEF2/Elongation factor 2 antibody (ab33523)
Predicted band size: 95 kDa
Western blot - Anti-EEF2/Elongation factor 2 antibody (ab33523)
All lanes: Western blot - Anti-EEF2/Elongation factor 2 antibody (ab33523) at 1 µg/mL
Lane 1: Marker
Lane 2: Zebrafish brain homogenate (20ug)
Lane 3: Zebrafish heart homogenate (20ug)
Lane 4: Zebrafish liver homogenate (20ug)
Lane 5: Zebrafish skeletal muscle homogenate (20ug)
Lane 6: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (20ug)
SecondaryAll lanes: Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 95 kDa
Observed band size: 95 kDa
Exposure time: 5min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2/Elongation factor 2 antibody (ab33523)
IHC image of EEF2/Elongation factor 2 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab33523, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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