AB53114

Anti-EEF2/Elongation factor 2 (phospho T56) antibody

Name: Anti-EEF2/Elongation factor 2 (phospho T56) antibody (ab53114) | Abcam
Brand: Abcam Limited
SKU: AB53114
Price: 510 USD
Availability: InStock

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(9 Publications)

Rabbit Polyclonal EEF2/Elongation factor 2 phospho T56 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human EEF2 phospho T56 aa 1-100.

View Alternative Names

EF2, EEF2, Elongation factor 2, EF-2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2/Elongation factor 2 (phospho T56) antibody (AB53114)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2/Elongation factor 2 (phospho T56) antibody (AB53114)

ab53114 at 1/50 dilution staining EEF2/Elongation factor 2 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.

Unknown

Western blot - Anti-EEF2/Elongation factor 2 (phospho T56) antibody (AB53114)

All lanes:

Western blot - Anti-EEF2/Elongation factor 2 (phospho T56) antibody (ab53114) at 1/300 dilution

Lane 1:

Extract from NIH 3T3 cells treated with serum (10%, 30mins) with Immunising peptide

Lane 2:

Extract from NIH 3T3 cells treated with serum (10%, 30mins)

Predicted band size: 95 kDa

Observed band size: 95 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human EEF2 phospho T56 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P13639

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/300 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab53114 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EEF2 or elongation factor 2 is an important protein in the process of translation during protein synthesis. It is also known by the names EF-2 and EF2. This protein facilitates the translocation step where it moves tRNA and mRNA from the A-site to the P-site on the ribosome. EEF2 has a molecular weight of approximately 95 kDa. The protein is ubiquitously expressed in various tissues and cells due to its fundamental role in protein synthesis making it essential for cellular activities.

Biological function summary

EEF2 functions as part of a larger ribosomal complex. It has an important role in ensuring the accuracy and efficiency of elongation during the translation process. This process is energy-dependent with EEF2 requiring GTP to catalyze the movement along the mRNA strand. The protein must undergo reversible phosphorylation which directly affects its activity. This post-translational modification of EEF2 is a significant modulation point influencing the rate of protein synthesis.

Pathways

EEF2 plays an integral role in the mTOR signaling pathway and the insulin signaling pathway. In the mTOR pathway EEF2 activity is regulated to control protein translation based on cellular energy status and nutrient availability. This pathway is important for cell growth and proliferation. EEF2 interacts with other proteins such as factor 2 and eEF2kinase which phosphorylates the protein thereby inhibiting its activity. In the insulin signaling pathway similar regulatory mechanisms impact EEF2’s role in translation.

EEF2 connects to cancer and neurodegenerative diseases. In cancer aberrant regulation of EEF2 can lead to uncontrolled protein synthesis promoting cell proliferation and tumor growth. Its interaction with mTOR and other signaling proteins like ribosomal proteins plays a role in this dysregulation. In neurodegenerative conditions such as Alzheimer’s disease impaired EEF2 activity results in decreased synaptic protein synthesis affecting neuronal function. Understanding EEF2’s regulation and dysregulation provides insight into its involvement in these diseases offering potential for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation (PubMed : 26593721). During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively (PubMed : 26593721). Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (PubMed : 26593721).

See full target information EEF2 phospho T56

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Acta pharmaceutica Sinica. B 15:3663-3684 PubMed40698138

2025

Cytoplasmic and nuclear NFATc3 cooperatively contributes to vascular smooth muscle cell dysfunction and drives aortic aneurysm and dissection.

Applications

Unspecified application

Species

Unspecified reactive species

Xiu Liu,Li Zhao,Deshen Liu,Lingna Zhao,Yonghua Tuo,Qinbao Peng,Fangze Huang,Zhengkun Song,Chuanjie Niu,Xiaoxia He,Yu Xu,Jun Wan,Peng Zhu,Zhengyang Jian,Jiawei Guo,Yingying Liu,Jun Lu,Sijia Liang,Shaoyi Zheng

Pharmacological reports : PR 76:1001-1011 PubMed39042346

2024

Subchronic administration of scopolamine reverses UCMS-induced behavior in mice via eEF2 protein dephosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Yana Babii,Agnieszka Pałucha-Poniewiera,Anna Rafało-Ulińska,Piotr Brański,Andrzej Pilc

Cancer cell 41:853-870.e13 PubMed37084735

2023

Transcriptional-translational conflict is a barrier to cellular transformation and cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Sujata Jana,Sandipan Brahma,Sonali Arora,Cynthia L Wladyka,Patrick Hoang,Steven Blinka,Rowan Hough,Jessie L Horn,Yuzhen Liu,Li-Jie Wang,Philippe Depeille,Eric Smith,Robert B Montgomery,John K Lee,Michael C Haffner,Funda Vakar-Lopez,Petros Grivas,Jonathan L Wright,Hung-Ming Lam,Peter C Black,Jeroen P Roose,Alexey G Ryazanov,Arvind R Subramaniam,Steven Henikoff,Andrew C Hsieh

Current biology : CB 30:3507-3521.e7 PubMed32707059

2020

eEF2/eEF2K Pathway in the Mature Dentate Gyrus Determines Neurogenesis Level and Cognition.

Applications

Unspecified application

Species

Unspecified reactive species

Elham Taha,Sudarshan Patil,Iliana Barrera,Julia Panov,Mohammad Khamaisy,Christopher G Proud,Clive R Bramham,Kobi Rosenblum

Journal of dairy science 102:4264-4274 PubMed30879806

2019

Phosphorylation of AKT serine/threonine kinase and abundance of milk protein synthesis gene networks in mammary tissue in response to supply of methionine in periparturient Holstein cows.

Applications

Unspecified application

Species

Unspecified reactive species

Y F Ma,F Batistel,T L Xu,L Q Han,R Bucktrout,Y Liang,D N Coleman,C Parys,J J Loor

Journal of cellular biochemistry : PubMed30450663

2018

Activation of the mammalian target of rapamycin signaling pathway underlies a novel inhibitory role of ring finger protein 182 in ventricular remodeling after myocardial ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jing-Hua Wang,Zhi-Feng Wei,Yan-Li Gao,Cong-Cong Liu,Jing-Hui Sun

Cell death and differentiation 24:1564-1576 PubMed28574509

2017

MYCN amplified neuroblastoma requires the mRNA translation regulator eEF2 kinase to adapt to nutrient deprivation.

Applications

Unspecified application

Species

Unspecified reactive species

Alberto Delaidelli,Gian Luca Negri,Asad Jan,Brandon Jansonius,Amal El-Naggar,Jonathan K M Lim,Debjit Khan,Htoo Zarni Oo,Christopher J Carnie,Marc Remke,John M Maris,Gabriel Leprivier,Poul H Sorensen

PloS one 8:e76508 PubMed24130777

2013

Hypoxia integration in the serological proteome analysis unmasks tumor antigens and fosters the identification of anti-phospho-eEF2 antibodies as potential cancer biomarkers.

Applications

WB, ICC/IF, ICC/IF, WB

Species

Human, Mouse, Human, Mouse

Marie Grandjean,Alexandra Sermeus,Samuel Branders,Florence Defresne,Marc Dieu,Pierre Dupont,Martine Raes,Mark De Ridder,Olivier Feron

Journal of proteomics 75:4555-69 PubMed22580036

2012

Identification and quantification of concentration-dependent biomarkers in MCF-7/BOS cells exposed to 17β-estradiol by 2-D DIGE and label-free proteomics.

Applications

Species

Human

Mike Collodoro,Pascale Lemaire,Gauthier Eppe,Virginie Bertrand,Rowan Dobson,Gabriel Mazzucchelli,Joëlle Widart,Edwin De Pauw,Marie-Claire De Pauw-Gillet

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-EEF2/Elongation factor 2 (phospho T56) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2/Elongation factor 2 (phospho T56) antibody (AB53114)

Western blot - Anti-EEF2/Elongation factor 2 (phospho T56) antibody (AB53114)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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