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Rabbit Multiclonal EEF2/Elongation factor 2 phospho T56 antibody. Suitable for WB and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human Elongation factor 2 phospho T56.

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Images

Western blot - Anti-EEF2/Elongation factor 2 (phospho T56) antibody [RP23040245] (AB308101), expandable thumbnail
  • Western blot - Anti-EEF2/Elongation factor 2 (phospho T56) antibody [RP23040245] (AB308101), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS

Form

Liquid

Clonality

Multiclonal

Immunogen

  • Synthetic Peptide within Human Elongation factor 2 phospho T56. Database link P13639

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested

Tested
Tested

Species

Mouse

Dilution info

2.5 µg/mL

Notes

-

Species

Human

Dilution info

2.5 µg/mL

Notes

-

Target data

Function

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation (PubMed:26593721). During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively (PubMed:26593721). Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (PubMed:26593721).

Alternative names

Recommended products

Rabbit Multiclonal EEF2/Elongation factor 2 phospho T56 antibody. Suitable for WB and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human Elongation factor 2 phospho T56.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Multiclonal

Immunogen
  • Synthetic Peptide within Human Elongation factor 2 phospho T56. Database link P13639
Clone number

RP23040245

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.

Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-EEF2/Elongation factor 2 (phospho T56) antibody [RP23040245] (ab308101), expandable thumbnail

    Western blot - Anti-EEF2/Elongation factor 2 (phospho T56) antibody [RP23040245] (ab308101)

    Western blot analysis was performed on Whole cell extracts (30 µg lysate) of Jurkat (Lane 1), T98G (Lane 2), Hep G2 (Lane 3), 3T3-L1 (Lane 4), 3T3-L1 treated with Forskolin (100uM for 60 mins) (Lane 5), NIH/3T3 (Lane 6), NIH/3T3 treated with Forskolin (100uM for 60 mins) (Lane 7). The blots were probed with ab308101 and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1:4000 dilution. A 95 kDa band corresponding to EEF2 (phospho T56) was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using a 4-12% Bis-Tris gel, electrophoresis system and pre-stained protein standard. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed (ECL).

    All lanes: Western blot - Anti-EEF2/Elongation factor 2 (phospho T56) antibody [RP23040245] (ab308101) at 2.5 µg/mL

    Lane 1: Jurkat cell lysate at 30 µg

    Lane 2: T98G cell lysate at 30 µg

    Lane 3: HepG2 cell lysate at 30 µg

    Lane 4: 3T3-L1 cell lysate at 30 µg

    Lane 5: Forskolin treated 3T3-L1 cell lysate at 30 µg

    Lane 6: NIH/3T3 cell lysate at 30 µg

    Lane 7: Forskolin treated NIH/3T3 cell lysate at 30 µg

    Secondary

    All lanes: HRP conjugated Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1/4000 dilution

    Developed using the ECL technique.

    Observed band size: 95 kDa

  • Western blot - Anti-EEF2/Elongation factor 2 (phospho T56) antibody [RP23040245] (ab308101), expandable thumbnail

    Western blot - Anti-EEF2/Elongation factor 2 (phospho T56) antibody [RP23040245] (ab308101)

    Western blot analysis was performed on Whole cell extracts (30 µg lysate) of NIH/3T3 (Lane 1) and NIH/3T3 treated with Forskolin (100uM for 60 mins) (Lane 2). The blots were probed with ab308101 and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1:4000 dilution. A 95 kDa band corresponding to EEF2 (phospho T56) was observed. Antibody specificity was demonstrated by competition with the EEF2 (phospho T56) peptide, which results in loss of signal. No competition was observed with the non-phospho peptide. Known quantity of protein samples were electrophoresed using a 4-12% Bis-Tris gel, electrophoresis system and pre-stained protein standard. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed (ECL).

    All lanes: Western blot - Anti-EEF2/Elongation factor 2 (phospho T56) antibody [RP23040245] (ab308101) at 2.5 µg/mL

    Lane 1: untreated NIH/3T3 cell lysate at 30 µg

    Lane 2: Forskolin treated NIH/3T3 cell lysate at 30 µg

    Lane 3: EEF2 (phospho T56) phosphopeptide incubated NIH/3T3 cell lysate at 30 µg

    Lane 4: EEF2 (phospho T56) phosphopeptide incubated Forskolin treated NIH/3T3 cell lysate at 30 µg

    Lane 5: non-phosphopeptide incubated NIH/3T3 cell lysate at 30 µg

    Lane 6: Non-phosphopeptide treated Forskolin treated NIH/3T3 cell lysate at 30 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/4000 dilution

    Developed using the ECL technique.

    Observed band size: 95 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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