Rabbit Multiclonal EEF2/Elongation factor 2 phospho T56 antibody. Suitable for WB and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human Elongation factor 2 phospho T56.
IgG
Rabbit
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS
Liquid
Multiclonal
WB | |
---|---|
Human | Tested |
Mouse | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 2.5 µg/mL | Notes - |
Species Human | Dilution info 2.5 µg/mL | Notes - |
Catalyzes the GTP-dependent ribosomal translocation step during translation elongation (PubMed:26593721). During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively (PubMed:26593721). Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (PubMed:26593721).
EF2, EEF2, EF2, Elongation factor 2, EF-2
Rabbit Multiclonal EEF2/Elongation factor 2 phospho T56 antibody. Suitable for WB and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human Elongation factor 2 phospho T56.
IgG
Rabbit
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS
Liquid
Multiclonal
RP23040245
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.
Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Western blot analysis was performed on Whole cell extracts (30 µg lysate) of Jurkat (Lane 1), T98G (Lane 2), Hep G2 (Lane 3), 3T3-L1 (Lane 4), 3T3-L1 treated with Forskolin (100uM for 60 mins) (Lane 5), NIH/3T3 (Lane 6), NIH/3T3 treated with Forskolin (100uM for 60 mins) (Lane 7). The blots were probed with ab308101 and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1:4000 dilution. A 95 kDa band corresponding to EEF2 (phospho T56) was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using a 4-12% Bis-Tris gel, electrophoresis system and pre-stained protein standard. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed (ECL).
All lanes: Western blot - Anti-EEF2/Elongation factor 2 (phospho T56) antibody [RP23040245] (ab308101) at 2.5 µg/mL
Lane 1: Jurkat cell lysate at 30 µg
Lane 2: T98G cell lysate at 30 µg
Lane 3: HepG2 cell lysate at 30 µg
Lane 4: 3T3-L1 cell lysate at 30 µg
Lane 5: Forskolin treated 3T3-L1 cell lysate at 30 µg
Lane 6: NIH/3T3 cell lysate at 30 µg
Lane 7: Forskolin treated NIH/3T3 cell lysate at 30 µg
All lanes: HRP conjugated Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1/4000 dilution
Developed using the ECL technique.
Observed band size: 95 kDa
Western blot analysis was performed on Whole cell extracts (30 µg lysate) of NIH/3T3 (Lane 1) and NIH/3T3 treated with Forskolin (100uM for 60 mins) (Lane 2). The blots were probed with ab308101 and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1:4000 dilution. A 95 kDa band corresponding to EEF2 (phospho T56) was observed. Antibody specificity was demonstrated by competition with the EEF2 (phospho T56) peptide, which results in loss of signal. No competition was observed with the non-phospho peptide. Known quantity of protein samples were electrophoresed using a 4-12% Bis-Tris gel, electrophoresis system and pre-stained protein standard. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed (ECL).
All lanes: Western blot - Anti-EEF2/Elongation factor 2 (phospho T56) antibody [RP23040245] (ab308101) at 2.5 µg/mL
Lane 1: untreated NIH/3T3 cell lysate at 30 µg
Lane 2: Forskolin treated NIH/3T3 cell lysate at 30 µg
Lane 3: EEF2 (phospho T56) phosphopeptide incubated NIH/3T3 cell lysate at 30 µg
Lane 4: EEF2 (phospho T56) phosphopeptide incubated Forskolin treated NIH/3T3 cell lysate at 30 µg
Lane 5: non-phosphopeptide incubated NIH/3T3 cell lysate at 30 µg
Lane 6: Non-phosphopeptide treated Forskolin treated NIH/3T3 cell lysate at 30 µg
All lanes: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/4000 dilution
Developed using the ECL technique.
Observed band size: 95 kDa
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