AB82981

Anti-EEF2/Elongation factor 2 (phospho T56 + T58) antibody [EP833Y]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(6 Publications )

Rabbit Recombinant Monoclonal EEF2/Elongation factor 2 phospho T56 + T58 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

1 Images

Unknown

Western blot - Anti-EEF2/Elongation factor 2 (phospho T56 + T58) antibody [EP833Y] (AB82981)

All lanes:

Western blot - Anti-EEF2/Elongation factor 2 (phospho T56 + T58) antibody [EP833Y] (ab82981) at 1/2000 dilution

Lane 1:

Untreated 293 cell lysate at 10 µg

Lane 2:

Serum starved 293 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 95 kDa

Observed band size: 95 kDa

false

Carrier free

Anti-EEF2/Elongation factor 2 (phospho T56 + T58) antibody [EP833Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP833Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EEF2 or elongation factor 2 is an important protein in the process of translation during protein synthesis. It is also known by the names EF-2 and EF2. This protein facilitates the translocation step where it moves tRNA and mRNA from the A-site to the P-site on the ribosome. EEF2 has a molecular weight of approximately 95 kDa. The protein is ubiquitously expressed in various tissues and cells due to its fundamental role in protein synthesis making it essential for cellular activities.

Biological function summary

EEF2 functions as part of a larger ribosomal complex. It has an important role in ensuring the accuracy and efficiency of elongation during the translation process. This process is energy-dependent with EEF2 requiring GTP to catalyze the movement along the mRNA strand. The protein must undergo reversible phosphorylation which directly affects its activity. This post-translational modification of EEF2 is a significant modulation point influencing the rate of protein synthesis.

Pathways

EEF2 plays an integral role in the mTOR signaling pathway and the insulin signaling pathway. In the mTOR pathway EEF2 activity is regulated to control protein translation based on cellular energy status and nutrient availability. This pathway is important for cell growth and proliferation. EEF2 interacts with other proteins such as factor 2 and eEF2kinase which phosphorylates the protein thereby inhibiting its activity. In the insulin signaling pathway similar regulatory mechanisms impact EEF2’s role in translation.

EEF2 connects to cancer and neurodegenerative diseases. In cancer aberrant regulation of EEF2 can lead to uncontrolled protein synthesis promoting cell proliferation and tumor growth. Its interaction with mTOR and other signaling proteins like ribosomal proteins plays a role in this dysregulation. In neurodegenerative conditions such as Alzheimer’s disease impaired EEF2 activity results in decreased synaptic protein synthesis affecting neuronal function. Understanding EEF2’s regulation and dysregulation provides insight into its involvement in these diseases offering potential for therapeutic intervention.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation (PubMed : 26593721). During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively (PubMed : 26593721). Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (PubMed : 26593721).

See full target information EEF2 pT56 + T58

Alternative Names

EF2, EEF2, Elongation factor 2, EF-2

style

left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cell discovery 11:58 PubMed40555714

2025

Sleep prevents brain phosphoproteome disruption to safeguard survival.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Ma,Juhang Liu,Yu Li,Yikui Zhao,Yu Tian,Bing Hu,Kaiyue Yan,Ying Li,Kaihang Ding,Xiangyu Wang,Huiwen Tian,Wen Si,Ketong Liu,Huiran Zhang,Chongchong Zhao,Guangfu Wang,Zhiqiang Wang

Science translational medicine 11: PubMed31619542

2019

Mutant neuropeptide S receptor reduces sleep duration with preserved memory consolidation.

Applications

Unspecified application

Species

Unspecified reactive species

Lijuan Xing,Guangsen Shi,Yulia Mostovoy,Nicholas W Gentry,Zenghua Fan,Thomas B McMahon,Pui-Yan Kwok,Christopher R Jones,Louis J Ptáček,Ying-Hui Fu

Nature 558:435-439 PubMed29899451

2018

Quantitative phosphoproteomic analysis of the molecular substrates of sleep need.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiqiang Wang,Jing Ma,Chika Miyoshi,Yuxin Li,Makito Sato,Yukino Ogawa,Tingting Lou,Chengyuan Ma,Xue Gao,Chiyu Lee,Tomoyuki Fujiyama,Xiaojie Yang,Shuang Zhou,Noriko Hotta-Hirashima,Daniela Klewe-Nebenius,Aya Ikkyu,Miyo Kakizaki,Satomi Kanno,Liqin Cao,Satoru Takahashi,Junmin Peng,Yonghao Yu,Hiromasa Funato,Masashi Yanagisawa,Qinghua Liu

The Journal of biological chemistry 289:23901-16 PubMed25012662

2014

The molecular mechanism of eukaryotic elongation factor 2 kinase activation.

Applications

Unspecified application

Species

Human

Clint D J Tavares,Scarlett B Ferguson,David H Giles,Qiantao Wang,Rebecca M Wellmann,John P O'Brien,Mangalika Warthaka,Jennifer S Brodbelt,Pengyu Ren,Kevin N Dalby

The Journal of physiology 589:3623-40 PubMed21606113

2011

Dihydrotestosterone stimulates amino acid uptake and the expression of LAT2 in mouse skeletal muscle fibres through an ERK1/2-dependent mechanism.

Applications

Species

Mouse

M M Hamdi,G Mutungi

Biochimica et biophysica acta 1814:850-7 PubMed21513824

2011

An analysis of the phosphoproteome of immune cell lines exposed to the immunomodulatory mycotoxin deoxynivalenol.

Applications

Unspecified application

Species

Unspecified reactive species

André Nogueira da Costa,Jeffrey N Keen,Christopher P Wild,John B C Findlay

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EEF2/Elongation factor 2 (phospho T56 + T58) antibody [EP833Y]

Western blot - Anti-EEF2/Elongation factor 2 (phospho T56 + T58) antibody [EP833Y] (AB82981)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (6)

Product promise