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AB85721

Anti-EEF2K antibody

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(4 Publications)

Rabbit Polyclonal EEF2K antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human Eukaryotic elongation factor 2 kinase aa 1-50.

View Alternative Names

Eukaryotic elongation factor 2 kinase, eEF-2 kinase, eEF-2K, Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase, EEF2K

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2K antibody (AB85721)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2K antibody (AB85721)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling EEF2K with ab85721 at 1/1000 (1µg/ml). Detection : DAB.

Western blot - Anti-EEF2K antibody (AB85721)
  • WB

Unknown

Western blot - Anti-EEF2K antibody (AB85721)

All lanes:

Western blot - Anti-EEF2K antibody (ab85721) at 0.1 µg/mL

Lane 1:

HeLa cells lysate at 50 µg

Lane 2:

HeLa cells lysate at 15 µg

Lane 3:

HeLa cells lysate at 5 µg

Predicted band size: 82 kDa

Observed band size: 110 kDa,350 kDa,95 kDa

true

Exposure time: 3min

Immunoprecipitation - Anti-EEF2K antibody (AB85721)
  • IP

Unknown

Immunoprecipitation - Anti-EEF2K antibody (AB85721)

Detection of Human EEF2K by Immunoprecipitation in HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab85721 at 3 ug/mg lysate. Subsequent Western blot detection was performed using 1 ug/ml ab85721. Detection : Chemiluminescence with exposure times of 30 seconds.

All lanes:

Immunoprecipitation - Anti-EEF2K antibody (ab85721)

Predicted band size: 82 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human Eukaryotic elongation factor 2 kinase aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

O00418

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EEF2K also known as eukaryotic elongation factor 2 kinase or CaMKIII is a significant protein kinase with an approximate molecular mass of 85 kDa. EEF2K plays a role in controlling protein synthesis by phosphorylating eukaryotic elongation factor 2 (eEF2) leading to a decrease in its activity. This protein can be found in various tissues throughout the body but shows substantial expression in the brain. It acts as a regulatory switch in translation process under specific cellular conditions.
Biological function summary

EEF2K regulates protein translation and affects cellular energy expenditure during conditions such as starvation or stress. Through its activity EEF2K contributes to the cellular response by phosphorylating eEF2 resulting in modulation of ribosomal function. Although EEF2K does not necessarily form stable complexes with other proteins its function influences several other cellular activities that are in connection with protein synthesis and degradation pathways.

Pathways

EEF2K integrates into nutrient-signaling pathways like the mTOR signaling and the AMPK pathway. These pathways control energy homeostasis and response to cellular stress. EEF2K acts alongside AMPK when nutrient supply is low adjusting protein synthesis rates to meet cellular energy demands. Additionally EEF2K's function inversely relates to mTOR activity which typically promotes protein synthesis and cell growth when conditions are favorable.

EEF2K associates with cancer and neurodegenerative diseases. In cancer EEF2K modulates cell survival under nutrient-poor environments often contributing to chemotherapy resistance. Links between EEF2K and neurodegenerative diseases come from its regulation of protein synthesis which affects neuronal health. Proteins like AMPK connect to EEF2K highlighting their collective contribution to the metabolic rearrangements seen in these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.
See full target information Eukaryotic elongation factor 2 kinase
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2412985 PubMed39950798

2025

Identification of a Novel Substrate for eEF2K and the AURKA-SOX8 as the Related Pathway in TNBC.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoya Wan,Rong Gong,Xiaobao Zhao,Yizhi Li,Tianjiao Shan,Changxin Zhong,Rongfeng Zhu,Zonglin Chen,Shilong Jiang,Linhao He,Shijun Cao,Sheng Tian,Jinming Yang,Na Ye,Wenjun Yi,Yan Cheng

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2305035 PubMed38084501

2023

Design and Characterization of a Novel eEF2K Degrader with Potent Therapeutic Efficacy Against Triple-Negative Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Changxin Zhong,Rongfeng Zhu,Ting Jiang,Sheng Tian,Xiaobao Zhao,Xiaoya Wan,Shilong Jiang,Zonglin Chen,Rong Gong,Linhao He,Jin-Ming Yang,Na Ye,Yan Cheng

Journal for immunotherapy of cancer 10: PubMed35347072

2022

eEF2K promotes PD-L1 stabilization through inactivating GSK3β in melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xisha Chen,Kuansong Wang,Shilong Jiang,Hongyin Sun,Xuanling Che,Minghui Zhang,Jiaying He,Ying Wen,Mengting Liao,Xiangling Li,Xiaoming Zhou,Jianxun Song,Xingcong Ren,Wenjun Yi,Jinming Yang,Xiang Chen,Mingzhu Yin,Yan Cheng

Nature communications 9:2450 PubMed29934589

2018

TLR9 activation via microglial glucocorticoid receptors contributes to degeneration of midbrain dopamine neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Layal Maatouk,Anne-Claire Compagnion,Maria-Angeles Carrillo-de Sauvage,Alexis-Pierre Bemelmans,Sabrina Leclere-Turbant,Vincent Cirotteau,Mira Tohme,Allen Beke,Michaël Trichet,Virginie Bazin,Bobby N Trawick,Richard M Ransohoff,François Tronche,Bénédicte Manoury,Sheela Vyas
View all publications
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