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AB46787

Anti-EEF2K antibody [EP724Y]

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(3 Publications)

Rabbit Monoclonal EEF2K antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

Eukaryotic elongation factor 2 kinase, eEF-2 kinase, eEF-2K, Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase, EEF2K

2 Images
Western blot - Anti-EEF2K antibody [EP724Y] (AB46787)
  • WB

Lab

Western blot - Anti-EEF2K antibody [EP724Y] (AB46787)

Western blot : Rabbit Monoclonal[EP724Y] to EEF2K ab46787 staining at 1/50000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta.

A band was observed at 82 kDa in Wild-type A549 cell lysates with no signal observed at this size in EEF2K knockout A549 cell line.

To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.

Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-EEF2K antibody [EP724Y] (ab46787) at 1/50000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

EEF2K knockout A549 at 20 µg

Lane 3:

Raji at 20 µg

Lane 4:

MOLT-4 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 82 kDa

Observed band size: 82 kDa

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Western blot - Anti-EEF2K antibody [EP724Y] (AB46787)
  • WB

Unknown

Western blot - Anti-EEF2K antibody [EP724Y] (AB46787)

All lanes:

Western blot - Anti-EEF2K antibody [EP724Y] (ab46787) at 1/50000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 82 kDa

Observed band size: 95 kDa

false

  • Carrier free

    Anti-EEF2K antibody [EP724Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP724Y

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 99% Tissue culture supernatant, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EEF2K also known as eukaryotic elongation factor 2 kinase or CaMKIII is a significant protein kinase with an approximate molecular mass of 85 kDa. EEF2K plays a role in controlling protein synthesis by phosphorylating eukaryotic elongation factor 2 (eEF2) leading to a decrease in its activity. This protein can be found in various tissues throughout the body but shows substantial expression in the brain. It acts as a regulatory switch in translation process under specific cellular conditions.
Biological function summary

EEF2K regulates protein translation and affects cellular energy expenditure during conditions such as starvation or stress. Through its activity EEF2K contributes to the cellular response by phosphorylating eEF2 resulting in modulation of ribosomal function. Although EEF2K does not necessarily form stable complexes with other proteins its function influences several other cellular activities that are in connection with protein synthesis and degradation pathways.

Pathways

EEF2K integrates into nutrient-signaling pathways like the mTOR signaling and the AMPK pathway. These pathways control energy homeostasis and response to cellular stress. EEF2K acts alongside AMPK when nutrient supply is low adjusting protein synthesis rates to meet cellular energy demands. Additionally EEF2K's function inversely relates to mTOR activity which typically promotes protein synthesis and cell growth when conditions are favorable.

EEF2K associates with cancer and neurodegenerative diseases. In cancer EEF2K modulates cell survival under nutrient-poor environments often contributing to chemotherapy resistance. Links between EEF2K and neurodegenerative diseases come from its regulation of protein synthesis which affects neuronal health. Proteins like AMPK connect to EEF2K highlighting their collective contribution to the metabolic rearrangements seen in these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.
See full target information Eukaryotic elongation factor 2 kinase
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:501 PubMed39003251

2024

The eEF2 kinase coordinates the DNA damage response to cisplatin by supporting p53 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan K M Lim,Arash Samiei,Alberto Delaidelli,Jessica Oliveira de Santis,Vanessa Brinkmann,Christopher J Carnie,Daniel Radiloff,Laura Hruby,Alisa Kahler,Jordan Cran,Gabriel Leprivier,Poul H Sorensen

Cancer cell 41:853-870.e13 PubMed37084735

2023

Transcriptional-translational conflict is a barrier to cellular transformation and cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Sujata Jana,Sandipan Brahma,Sonali Arora,Cynthia L Wladyka,Patrick Hoang,Steven Blinka,Rowan Hough,Jessie L Horn,Yuzhen Liu,Li-Jie Wang,Philippe Depeille,Eric Smith,Robert B Montgomery,John K Lee,Michael C Haffner,Funda Vakar-Lopez,Petros Grivas,Jonathan L Wright,Hung-Ming Lam,Peter C Black,Jeroen P Roose,Alexey G Ryazanov,Arvind R Subramaniam,Steven Henikoff,Andrew C Hsieh

Acta neuropathologica communications 6:54 PubMed29961428

2018

Activity of translation regulator eukaryotic elongation factor-2 kinase is increased in Parkinson disease brain and its inhibition reduces alpha synuclein toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Asad Jan,Brandon Jansonius,Alberto Delaidelli,Forum Bhanshali,Yi Andy An,Nelson Ferreira,Lisa M Smits,Gian Luca Negri,Jens C Schwamborn,Poul H Jensen,Ian R Mackenzie,Stefan Taubert,Poul H Sorensen
View all publications
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