Rabbit Monoclonal EEF2K antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.
Images
![Western blot - Anti-EEF2K antibody [EP724Y] (AB46787), expandable thumbnail](https://content.abcam.com/products/images/eef2k-antibody-ep724y-ab46787--western-blot-img58779.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-EEF2K antibody [EP724Y] (AB46787)")
![Western blot - Anti-EEF2K antibody [EP724Y] (AB46787), expandable thumbnail](https://content.abcam.com/products/images/eef2k-antibody-ep724y-ab46787--western-blot-img72217.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-EEF2K antibody [EP724Y] (AB46787)")
Publications
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- Acta neuropathologica communications 6:542018Activity of translation regulator eukaryotic elongation factor-2 kinase is increased in Parkinson disease brain and its inhibition reduces alpha synuclein toxicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAsad Jan et. al.PubMed 29961428
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Predicted | Not recommended |
| Rat | Not recommended | Not recommended | Predicted | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.
Alternative names
Eukaryotic elongation factor 2 kinase, eEF-2 kinase, eEF-2K, Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase, EEF2K
Recommended products
Rabbit Monoclonal EEF2K antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP724Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EEF2K also known as eukaryotic elongation factor 2 kinase or CaMKIII is a significant protein kinase with an approximate molecular mass of 85 kDa. EEF2K plays a role in controlling protein synthesis by phosphorylating eukaryotic elongation factor 2 (eEF2) leading to a decrease in its activity. This protein can be found in various tissues throughout the body but shows substantial expression in the brain. It acts as a regulatory switch in translation process under specific cellular conditions.
- Biological function summary
EEF2K regulates protein translation and affects cellular energy expenditure during conditions such as starvation or stress. Through its activity EEF2K contributes to the cellular response by phosphorylating eEF2 resulting in modulation of ribosomal function. Although EEF2K does not necessarily form stable complexes with other proteins its function influences several other cellular activities that are in connection with protein synthesis and degradation pathways.
- Pathways
EEF2K integrates into nutrient-signaling pathways like the mTOR signaling and the AMPK pathway. These pathways control energy homeostasis and response to cellular stress. EEF2K acts alongside AMPK when nutrient supply is low adjusting protein synthesis rates to meet cellular energy demands. Additionally EEF2K's function inversely relates to mTOR activity which typically promotes protein synthesis and cell growth when conditions are favorable.
- Associated diseases and disorders
EEF2K associates with cancer and neurodegenerative diseases. In cancer EEF2K modulates cell survival under nutrient-poor environments often contributing to chemotherapy resistance. Links between EEF2K and neurodegenerative diseases come from its regulation of protein synthesis which affects neuronal health. Proteins like AMPK connect to EEF2K highlighting their collective contribution to the metabolic rearrangements seen in these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
![Western blot - Anti-EEF2K antibody [EP724Y] (ab46787), expandable thumbnail](https://content.abcam.com/products/images/eef2k-antibody-ep724y-ab46787--western-blot-img58779.jpg "Western blot - Anti-EEF2K antibody [EP724Y] (ab46787)")
Western blot - Anti-EEF2K antibody [EP724Y] (ab46787)
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: EEF2K knockout HAP1 cell lysate (20 μg)
Lane 3: HeLa cell lysate (20 μg)
Lane 4: Jurkat cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - ab46787 observed at 95 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
ab46787 was shown to specifically react with EEF2K when EEF2K knockout samples were used. Wild-type and EEF2K knockout samples were subjected to SDS-PAGE. ab46787 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted at 1/50 000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.All lanes: Western blot - Anti-EEF2K antibody [EP724Y] (ab46787)
Predicted band size: 82 kDa
![Western blot - Anti-EEF2K antibody [EP724Y] (ab46787), expandable thumbnail](https://content.abcam.com/products/images/eef2k-antibody-ep724y-ab46787--western-blot-img72217.jpg "Western blot - Anti-EEF2K antibody [EP724Y] (ab46787)")
Western blot - Anti-EEF2K antibody [EP724Y] (ab46787)
All lanes: Western blot - Anti-EEF2K antibody [EP724Y] (ab46787) at 1/50000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: Jurkat cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 82 kDa
Observed band size: 95 kDa
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