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Rabbit Monoclonal EEF2K antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications.

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Images

Western blot - Anti-EEF2K antibody [EP881Y] (AB45168), expandable thumbnail
  • Western blot - Anti-EEF2K antibody [EP881Y] (AB45168), expandable thumbnail
  • Western blot - Anti-EEF2K antibody [EP881Y] (AB45168), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWB
Human
Not recommended
Tested
Mouse
Not recommended
Predicted
Rat
Not recommended
Predicted

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.

Alternative names

Eukaryotic elongation factor 2 kinase, eEF-2 kinase, eEF-2K, Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase, EEF2K

Recommended products

Rabbit Monoclonal EEF2K antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP881Y
Purity
Tissue culture supernatant
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

EEF2K also known as eukaryotic elongation factor 2 kinase or CaMKIII is a significant protein kinase with an approximate molecular mass of 85 kDa. EEF2K plays a role in controlling protein synthesis by phosphorylating eukaryotic elongation factor 2 (eEF2) leading to a decrease in its activity. This protein can be found in various tissues throughout the body but shows substantial expression in the brain. It acts as a regulatory switch in translation process under specific cellular conditions.

Biological function summary

EEF2K regulates protein translation and affects cellular energy expenditure during conditions such as starvation or stress. Through its activity EEF2K contributes to the cellular response by phosphorylating eEF2 resulting in modulation of ribosomal function. Although EEF2K does not necessarily form stable complexes with other proteins its function influences several other cellular activities that are in connection with protein synthesis and degradation pathways.

Pathways

EEF2K integrates into nutrient-signaling pathways like the mTOR signaling and the AMPK pathway. These pathways control energy homeostasis and response to cellular stress. EEF2K acts alongside AMPK when nutrient supply is low adjusting protein synthesis rates to meet cellular energy demands. Additionally EEF2K's function inversely relates to mTOR activity which typically promotes protein synthesis and cell growth when conditions are favorable.

Associated diseases and disorders

EEF2K associates with cancer and neurodegenerative diseases. In cancer EEF2K modulates cell survival under nutrient-poor environments often contributing to chemotherapy resistance. Links between EEF2K and neurodegenerative diseases come from its regulation of protein synthesis which affects neuronal health. Proteins like AMPK connect to EEF2K highlighting their collective contribution to the metabolic rearrangements seen in these disorders.

Product promise

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3 product images

  • Western blot - Anti-EEF2K antibody [EP881Y] (ab45168), expandable thumbnail

    Western blot - Anti-EEF2K antibody [EP881Y] (ab45168)

    This WB data was generated using the same anti-EEF2K antibody clone, EP881Y, in a different buffer formulation (cat# ab45168).

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: EEF2K knockout HAP1 cell lysate (20 μg)
    Lane 3: HeLa cell lysate (20 μg)
    Lane 4: Jurkat cell lysate (20 μg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab45168 observed at 95 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
    ab45168 was shown to specifically react with EEF2K when EEF2K knockout samples were used. Wild-type and EEF2K knockout samples were subjected to SDS-PAGE. ab45168 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    Lanes 1 - 4: Anti-EEF2K antibody [EP881Y] - BSA and Azide free (ab235390) at 1/1000 dilution

    Lanes 1 - 4: Western blot - Anti-EEF2K antibody [EP881Y] (ab45168) at 1/1000 dilution

    Predicted band size: 82 kDa

  • Western blot - Anti-EEF2K antibody [EP881Y] (ab45168), expandable thumbnail

    Western blot - Anti-EEF2K antibody [EP881Y] (ab45168)

    All lanes: Western blot - Anti-EEF2K antibody [EP881Y] (ab45168) at 1/1000 dilution

    All lanes: HEK293 cell lysate

    Predicted band size: 82 kDa

    Observed band size: 95 kDa

  • Western blot - Anti-EEF2K antibody [EP881Y] (ab45168), expandable thumbnail

    Western blot - Anti-EEF2K antibody [EP881Y] (ab45168)

    EEF2K Western blot staining using rabbit Anti-EEF2K antibody

    Western blot: Rabbit Monoclonal[EP881Y] to EEF2K ab45168 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 82 kDa in Wild-type A549 cell lysates with no signal observed at this size in EEF2K knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

    All lanes: Western blot - Anti-EEF2K antibody [EP881Y] (ab45168) at 1/1000 dilution

    Lane 1: Wild-type A549 at 20 µg

    Lane 2: EEF2K knockout A549 at 20 µg

    Lane 3: Raji at 20 µg

    Lane 4: MOLT-4 at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 82 kDa

    Observed band size: 82 kDa

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Product protocols

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