Anti-EEF2K antibody [EP881Y]

• WB

Unknown

Western blot - Anti-EEF2K antibody [EP881Y] (AB45168)

All lanes:

Western blot - Anti-EEF2K antibody [EP881Y] (ab45168) at 1/1000 dilution

All lanes:

HEK293 cell lysate

Predicted band size: 82 kDa

Observed band size: 95 kDa

false

• WB

Lab

Western blot - Anti-EEF2K antibody [EP881Y] (AB45168)

Western blot : Rabbit Monoclonal[EP881Y] to EEF2K ab45168 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 82 kDa in Wild-type A549 cell lysates with no signal observed at this size in EEF2K knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-EEF2K antibody [EP881Y] (ab45168) at 1/1000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

EEF2K knockout A549 at 20 µg

Lane 3:

Raji at 20 µg

Lane 4:

MOLT-4 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 82 kDa

Observed band size: 82 kDa

false