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AB51227

Anti-EEF2K (phospho S366) antibody

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Rabbit Polyclonal EEF2K phospho S366 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human Eukaryotic elongation factor 2 kinase phospho S366 aa 300-400.

View Alternative Names

Eukaryotic elongation factor 2 kinase, eEF-2 kinase, eEF-2K, Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase, EEF2K

2 Images
Western blot - Anti-EEF2K (phospho S366) antibody (AB51227)
  • WB

Unknown

Western blot - Anti-EEF2K (phospho S366) antibody (AB51227)

All lanes:

Western blot - Anti-EEF2K (phospho S366) antibody (ab51227) at 1/300 dilution

Lane 1:

Extracts of Hela cells treated with 10% serum for 15mins

Lane 2:

Extracts of Hela cells treated with 10% serum for 15mins and phosphopeptide

Predicted band size: 82 kDa

Observed band size: 100 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2K (phospho S366) antibody (AB51227)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2K (phospho S366) antibody (AB51227)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab51227 (right picture is blocked with the phospho peptide)

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human Eukaryotic elongation factor 2 kinase phospho S366 aa 300-400. The exact immunogen used to generate this antibody is proprietary information.

O00418

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EEF2K also known as eukaryotic elongation factor 2 kinase or CaMKIII is a significant protein kinase with an approximate molecular mass of 85 kDa. EEF2K plays a role in controlling protein synthesis by phosphorylating eukaryotic elongation factor 2 (eEF2) leading to a decrease in its activity. This protein can be found in various tissues throughout the body but shows substantial expression in the brain. It acts as a regulatory switch in translation process under specific cellular conditions.
Biological function summary

EEF2K regulates protein translation and affects cellular energy expenditure during conditions such as starvation or stress. Through its activity EEF2K contributes to the cellular response by phosphorylating eEF2 resulting in modulation of ribosomal function. Although EEF2K does not necessarily form stable complexes with other proteins its function influences several other cellular activities that are in connection with protein synthesis and degradation pathways.

Pathways

EEF2K integrates into nutrient-signaling pathways like the mTOR signaling and the AMPK pathway. These pathways control energy homeostasis and response to cellular stress. EEF2K acts alongside AMPK when nutrient supply is low adjusting protein synthesis rates to meet cellular energy demands. Additionally EEF2K's function inversely relates to mTOR activity which typically promotes protein synthesis and cell growth when conditions are favorable.

EEF2K associates with cancer and neurodegenerative diseases. In cancer EEF2K modulates cell survival under nutrient-poor environments often contributing to chemotherapy resistance. Links between EEF2K and neurodegenerative diseases come from its regulation of protein synthesis which affects neuronal health. Proteins like AMPK connect to EEF2K highlighting their collective contribution to the metabolic rearrangements seen in these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.
See full target information Eukaryotic elongation factor 2 kinase phospho S366
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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