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AB173528

Anti-EF-Ts antibody [EPR12034]

5

(1 Review)

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(2 Publications)

Rabbit Recombinant Monoclonal EF-Ts antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

EF-Ts, EF-TsMt, TSFM

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-EF-Ts antibody [EPR12034] (AB173528)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-EF-Ts antibody [EPR12034] (AB173528)

Immunofluorescence analysis of HeLa cells labeling EF-Ts with ab173528 at a 1/50 dilution.

Immunoprecipitation - Anti-EF-Ts antibody [EPR12034] (AB173528)
  • IP

Supplier Data

Immunoprecipitation - Anti-EF-Ts antibody [EPR12034] (AB173528)

Western blot analysis on immunoprecipitation pellet from 293T cell lysate : ab173528 at a 1/10 dilution was used to immunoprecipitated EF-Ts.

All lanes:

Immunoprecipitation - Anti-EF-Ts antibody [EPR12034] (ab173528)

Predicted band size: 35 kDa

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Western blot - Anti-EF-Ts antibody [EPR12034] (AB173528)
  • WB

Supplier Data

Western blot - Anti-EF-Ts antibody [EPR12034] (AB173528)

All lanes:

Western blot - Anti-EF-Ts antibody [EPR12034] (ab173528) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Predicted band size: 35 kDa

false

  • Carrier free

    Anti-EF-Ts antibody [EPR12034] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12034

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EF-Ts also known as Elongation Factor Ts is a protein with a molecular mass of approximately 30 kDa. It plays a significant role in protein synthesis specifically during the elongation phase of translation. EF-Ts is a guanine nucleotide exchange factor and primarily expressed in prokaryotic cells aiding EF-Tu in the exchange of GDP for GTP. By this function EF-Ts maintains a continuous supply of active EF-Tu essential for protein translation efficiency.
Biological function summary

EF-Ts facilitates the regeneration of its partner EF-Tu supporting its function in the ribosome. This protein forms part of the elongation factor complex an essential component of the translation machinery in bacterial cells. EF-Ts ensures EF-Tu remains in its active form enabling the delivery of aminoacyl-tRNA to the ribosomal A-site. Without EF-Ts EF-Tu would remain GDP-bound hampering protein synthesis.

Pathways

EF-Ts contributes to the translational control pathway by interacting directly with EF-Tu and ribosomes. It's important for maintaining the fidelity and speed of protein synthesis. EF-Ts operates alongside these components in the protein synthesis pathway which is central to cellular function and growth. EF-Ts does not work in isolation but coordinates closely with these other translational factors to maintain efficient protein production.

Dysfunctions in protein synthesis involving EF-Ts have implications for bacterial infections and antibiotic resistance. EF-Ts can be a target for antibiotics that aim to disrupt bacterial translation. Additionally studies have shown that mutations or alterations in related proteins like EF-Tu may lead to antibiotic resistance making EF-Ts a significant protein in understanding and combating bacterial diseases. Understanding EF-Ts' precise role offers insights into novel therapeutic approaches against these infections.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome.
See full target information TSFM
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Orphanet journal of rare diseases 17:204 PubMed35581596

2022

UPR activation improves pathological alterations in cellular models of mitochondrial diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Juan M Suárez-Rivero,Carmen J Pastor-Maldonado,Suleva Povea-Cabello,Mónica Álvarez-Córdoba,Irene Villalón-García,Marta Talaverón-Rey,Alejandra Suárez-Carrillo,Manuel Munuera-Cabeza,Diana Reche-López,Paula Cilleros-Holgado,Rocío Piñero-Perez,José A Sánchez-Alcázar

Physiological reports 6:e13893 PubMed30369085

2018

Exercise-induced mitochondrial biogenesis coincides with the expression of mitochondrial translation factors in murine skeletal muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Takumi Yokokawa,Kohei Kido,Tadashi Suga,Tadao Isaka,Tatsuya Hayashi,Satoshi Fujita
View all publications
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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