Rabbit Polyclonal EFCAB1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CLXN aa 100 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CLXN aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9HAE3
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10.00000 - 1/20.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Target data
Function
Component of the outer dynein arm-docking complex (ODA-DC) that mediates outer dynein arms (ODA) binding onto the doublet microtubule. Seems to regulate the assembly of both ODAs and their axonemal docking complex onto ciliary microtubules (By similarity). Regulates ciliary and flagellar motility and is required for cilia-driven determination of body laterality (By similarity).
Alternative names
EFCAB1, ODAD5, CLXN, Calaxin, EF-hand calcium-binding domain-containing protein 1
Recommended products
Rabbit Polyclonal EFCAB1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CLXN aa 100 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CLXN aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9HAE3
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EFCAB1 also known as EF-hand calcium-binding domain-containing protein 1 has an approximate mass of 32 kDa. This protein functions by interacting with calcium ions through its EF-hand domains which allows it to play a role in calcium signaling. EFCAB1 expression occurs in the testes and various other tissues indicating its relevance in multiple biological processes. Its calcium-binding capability suggests its involvement in processes where calcium acts as a secondary messenger.
- Biological function summary
The activities linked with EFCAB1 influence cellular signaling and regulation. It may associate with other cellular components to form complexes that adjust calcium dynamics therefore participating in signaling pathways that depend on calcium fluctuations. In doing so EFCAB1 aids in modulating activities that range from muscle contraction to neurotransmitter release exhibiting its versatility across physiological systems.
- Pathways
EFCAB1 may influence important biological pathways like calcium signaling and possibly the MAPK signaling pathway. Through such pathways EFCAB1 works alongside other proteins such as calmodulin and kinases to facilitate proper cellular responses to external stimuli. These interactions highlight EFCAB1’s contribution to maintaining cellular homeostasis and function through complex signaling cascades.
- Associated diseases and disorders
Altered function of EFCAB1 may connect to conditions such as idiopathic male infertility and certain types of cancers. The relationship between EFCAB1 and these disorders may be due to its role in calcium signaling as proper calcium homeostasis is critical for processes like spermatogenesis and cell proliferation. Proteins such as the protein kinase C family could link with EFCAB1 in these pathological contexts suggesting common pathways may contribute to disease development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-EFCAB1 antibody (ab121758)
All lanes: Western blot - Anti-EFCAB1 antibody (ab121758) at 1/250 dilution
Lane 1: vector only transfected HEK293T lysate
Lane 2: EFCAB1-transfected HEK293T lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))
Developed using the ECL technique.
Predicted band size: 24 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EFCAB1 antibody (ab121758)
ab1211758 at 1/15 dilution staining EFCAB1 in Paraffin-embedded Human duodenum tissue by Immunohistochemistry.
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